In Situ Forming Chitosan-Alginate Interpolymer Complex Bioplatform for Wound Healing and Regeneration
PUBLISHED IN: AAPS PharmSciTech 23, Article Number 247, (2022)
ABSTRACT
Cytocompatibility, biocompatibility, and biodegradability are amongst the most desirable qualities of wound dressings and can be tuned during the bioplatform fabrication steps to enhance wound healing capabilities. A three-stepped approach (partial-crosslinking, freeze-drying, and pulverisation) was employed in fabricating a particulate, partially crosslinked (PC), and transferulic acid (TFA)-loaded chitosan-alginate (CS-Alg) interpolymer complex (IPC) with enhanced wound healing capabilities. The PC TFA-CS-Alg IPC bioplatform displayed fluid uptake of 3102% in 24 h and a stepwise degradation up to 53.5% in 14 days. The PC TFA-CS-Alg bioplatform was used as a bioactive delivery system with an encapsulation efficiency of 65.6%, bioactive loading of 9.4%, burst release of 58.27%, and a steady release of 1.91% per day. PC TFA-CS-Alg displayed a shift in cytocompatibility from slightly cytotoxic (60–90% cell viability) to nontoxic (> 90% cell viability) over a 72-h period in NIH-3T3 cells. The wound closure and histological evaluations of the lesions indicated better wound healing performance in lesions treated with PC TFA-CS-Alg and PC CS-Alg compared to those treated with the commercial product and the control. Application of the particulate bioplatform on the wound via sprinkles, the in situ hydrogel formation under fluid exposure, and the accelerated wound healing performances of the bioplatforms make it a good candidate for bioactive delivery system and skin tissue regeneration.
Related Scientific Publications
Current treatments for glioblastoma (GBM) face challenges due to rapidly occurring tumor recurrences. In response, researchers have developed localized drug delivery systems, notably AT101-GlioMesh - an alginate-based mesh embedded with AT101-loaded PLGA microspheres. Fabricated for high encapsulation efficiency, this system ensures a sustained release of AT101, demonstrating a significant cytotoxic effect on GBM cell lines. This promising development could potentially revolutionize GBM therapy and prevent tumor recurrence.
Polysaccharide-based hydrogels offer great promise in 3D bioprinting due to their biocompatibility and cellular response, but their poor mechanical properties often require extensive crosslinking. The solution? Enter thermoresponsive bioinks. This study examines a triad of carboxymethyl cellulose, agarose, and gelatin as a potential thermoresponsive ink, demonstrating that specific blends can form stable hydrogels with desirable mechanical and physical properties. The bioinks' cytotoxicity was assessed on two cell lines according to ISO 10993-5 standards, and successful printing of complex 3D patterns confirmed their printability.
Dense collagen matrices, crafted through automated gel aspiration-ejection (GAE), offer exciting potential in the field of biofabrication. This study illuminates the crucial role fibrillization pH plays in both the real-time rheological changes during collagen hydrogel gelation and the properties of the resulting biofabricated matrices. Findings demonstrate a relative increase in hydrogel stiffness with higher gelation pH, with matrices demonstrating increased fibrillar density, alignment, and micro-compressive modulus at specific pH levels. Importantly, these matrices showed low cell mortality when seeded with fibroblasts. These findings may offer valuable insights applicable to other hydrogel systems and biofabrication techniques.
Understanding the biomechanical properties of arteries is complex due to their cylindrical shape and waveguide behavior. This study provides valuable insights by utilizing three-dimensional measurements on an artery-mimicking tube in water, categorizing the tube wall motion into transient and steady state responses. The study's approach enables a more accurate estimation of the motion and improves our understanding of wave propagation in arterial walls, presenting significant opportunities for enhanced measurement of arterial mechanical properties.
Researchers have devised a method called '3D wet writing' to create small-diameter arterial conduits. This technique uses ionic gelation for fabricating customizable constructs quickly and without a template. The constructs show mechanical properties similar to native blood vessels and demonstrate biocompatibility, indicating their potential use as vascular constructs.
A study shows a new kind of superabsorbent hydrogels (SAHs) that remain ionized at all physiological pH levels. The optimized SAHs show improved absorbency and stronger mechanics when swollen, making them potential contenders for commercial hygiene products.