{"id":70932,"date":"2026-09-29T13:51:25","date_gmt":"2026-09-29T17:51:25","guid":{"rendered":"https:\/\/rheolution.com\/?p=70932"},"modified":"2026-09-29T13:51:25","modified_gmt":"2026-09-29T17:51:25","slug":"medir-o-crescimento-de-levedura-em-um-biorreator-por-meio-da-turbididade","status":"publish","type":"post","link":"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/medir-o-crescimento-de-levedura-em-um-biorreator-por-meio-da-turbididade\/","title":{"rendered":"Como monitorar o crescimento de leveduras em um biorreator com o TURBIDI.T\u2122?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70932\" class=\"elementor elementor-70932 elementor-29296\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7eee7ee e-flex e-con-boxed e-con e-parent\" data-id=\"7eee7ee\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ea1afcb elementor-widget elementor-widget-heading\" data-id=\"ea1afcb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota de Aplica\u00e7\u00e3o | TURBIDI.T\u2122<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2141c30 elementor-widget elementor-widget-heading\" data-id=\"2141c30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Aprimorando o monitoramento do crescimento de leveduras por meio da medi\u00e7\u00e3o da turbidez\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e230945 elementor-widget elementor-widget-text-editor\" data-id=\"e230945\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"raw_components--panel--3IcXg inspect_panels--inspectionPanel--3Wboz\" tabindex=\"-1\"><div class=\"inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--propertyRowContent--7Y-mu inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--contentProperty--2Z1QI text--fontPos11--RSei3 text--_fontBase--YWDo0 ellipsis--ellipsisAfter8Lines--2rn32 ellipsis--_ellipsisAfterNLines--26JkZ\"><p>por <a href=\"https:\/\/www.linkedin.com\/in\/gloriapinilla\/\" target=\"_blank\" rel=\"noopener\">Gloria Pinilla<\/a> <span style=\"font-weight: 400;\">e <a href=\"https:\/\/www.linkedin.com\/in\/nora-kasaplar\" target=\"_blank\" rel=\"noopener\">Nora Kasaplar<\/a><\/span>,<\/p><p><span style=\"font-weight: 400;\">Cientistas de Aplica\u00e7\u00e3o<\/span><span style=\"font-weight: 400;\">,<\/span> <a href=\"https:\/\/www.linkedin.com\/company\/1168758\" target=\"_blank\" rel=\"noopener\">Rheolution Inc.<\/a><\/p><p>Em colabora\u00e7\u00e3o com  <\/p><p><em>Escola Nacional Superior de Tecnologia de Biomol\u00e9culas de Bordeaux (ENSTBB).<\/em><\/p><p><span id=\"docs-internal-guid-f0b2c698-7fff-5eae-53f6-94e076f7192c\" style=\"font-weight: normal;\"><span style=\"font-size: 10pt; font-family: Verdana, sans-serif; color: #002060; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space-collapse: preserve;\"><span style=\"border-width: initial; border-style: none; display: inline-block; overflow: hidden; width: 183px; height: 59px;\"><a href=\"https:\/\/enstbb.bordeaux-inp.fr\/fr\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/lh6.googleusercontent.com\/taVq6fxwO3y9M_Onk2MznWVYK4fGUswV4ylXbLTXiz--6Lnb5y_D8UU_0-BYZQ84LvhP4-Wzx6Je0H-TEH850xcAVrlCU1qZoUZuH2Wo37mQLyWOFqe-pn1FNScC6b94yI6cUA5K2fY2Yom1K9-EEAQ\" alt=\"\" width=\"183\" height=\"59\"><\/a><\/span><\/span><\/span><span style=\"letter-spacing: 0px;\"><br><\/span><\/p><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-35f268f elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"35f268f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resumo<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb634db elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"bb634db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>O monitoramento do crescimento de leveduras \u00e9 um processo crucial para garantir condi\u00e7\u00f5es ideais e produtividade nos processos biotecnol\u00f3gicos.  <\/li><li>As medi\u00e7\u00f5es baseadas na turbidez t\u00eam se destacado como uma abordagem r\u00e1pida e n\u00e3o destrutiva para estimar o crescimento de leveduras e acompanhar a densidade da cultura ao longo do tempo. <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">O TURBIDI.T\u2122<\/a> (Rheolution, QC, Canad\u00e1) tem sido utilizado para monitorar com precis\u00e3o o crescimento de <i>S. cerevisiae<\/i> ao longo do tempo.<\/li><li>Os resultados de turbidez (850 nm) e absorv\u00e2ncia (600 nm) foram comparados, demonstrando tend\u00eancias semelhantes e forte correla\u00e7\u00e3o.<\/li><li><a href=\"https:\/\/rheolution.com\/life-sciences\/products\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">O TURBIDI.T\u2122<\/a> demonstrou diversas vantagens em rela\u00e7\u00e3o ao espectrofot\u00f4metro no que diz respeito \u00e0 an\u00e1lise do crescimento celular e \u00e0 coleta de dados.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9453c51 e-flex e-con-boxed e-con e-parent\" data-id=\"9453c51\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5bdb09e elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5bdb09e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Avan\u00e7os no monitoramento do crescimento de leveduras: medi\u00e7\u00e3o da turbidez<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5965530 elementor-widget elementor-widget-heading\" data-id=\"5965530\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Introdu\u00e7\u00e3o<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b5ece66 elementor-widget elementor-widget-text-editor\" data-id=\"b5ece66\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>No campo da bioengenharia e da biotecnologia, as c\u00e9lulas atuam como catalisadoras na produ\u00e7\u00e3o de diversos produtos valiosos, tais como biocombust\u00edveis, produtos farmac\u00eauticos, anticorpos, enzimas industriais e produtos qu\u00edmicos de base biol\u00f3gica [1]. Nesse contexto, a levedura, um microrganismo amplamente utilizado, desempenha um papel fundamental. O monitoramento do crescimento da levedura \u00e9 de extrema import\u00e2ncia para garantir condi\u00e7\u00f5es ideais e produtividade nos processos biotecnol\u00f3gicos [2].  <\/p><p>Tradicionalmente, a an\u00e1lise espectrofotom\u00e9trica tem sido utilizada para avaliar a densidade \u00f3ptica a 600 nm (OD600nm) por meio da medi\u00e7\u00e3o da absor\u00e7\u00e3o de luz pela cultura de levedura, bem como para monitorar a evolu\u00e7\u00e3o da turbidez de uma popula\u00e7\u00e3o celular. As medi\u00e7\u00f5es baseadas na turbidez surgiram como uma abordagem r\u00e1pida e n\u00e3o destrutiva para estimar o crescimento da levedura e acompanhar a densidade da cultura ao longo do tempo. Essa metodologia aproveita os princ\u00edpios da dispers\u00e3o da luz, nos quais as mudan\u00e7as na concentra\u00e7\u00e3o e no tamanho das c\u00e9lulas influenciam a intensidade da luz dispersada pela cultura. Ao quantificar a turbidez resultante, \u00e9 poss\u00edvel obter informa\u00e7\u00f5es valiosas sobre a progress\u00e3o do crescimento celular.   <\/p><p><em>A Saccharomyces cerevisiae<\/em> (5-10 \u00b5m), tamb\u00e9m conhecida como levedura de padeiro ou levedura de cerveja, ocupa uma posi\u00e7\u00e3o de destaque como organismo-modelo no campo da produ\u00e7\u00e3o industrial. Isso se deve \u00e0 sua natureza bem caracterizada, robustez, alta produtividade e versatilidade.   <\/p><p>Esta nota de aplica\u00e7\u00e3o enfoca o uso do <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\">TURBIDI.T\u2122<\/a> (Rheolution, QC, Canad\u00e1) para monitorar o crescimento de <em>S. cerevisiae<\/em> ao longo do tempo. Os resultados obtidos s\u00e3o comparados e correlacionados com medi\u00e7\u00f5es de OD600 nm realizadas por meio de um espectrofot\u00f4metro padr\u00e3o. Ao analisar essa compara\u00e7\u00e3o, \u00e9 poss\u00edvel obter informa\u00e7\u00f5es valiosas para compreender melhor a efic\u00e1cia e a confiabilidade do <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\">TURBIDI.T\u2122<\/a> como ferramenta de monitoramento do crescimento de leveduras.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-62f3970 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"62f3970\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Monitoramento do crescimento de leveduras por meio da medi\u00e7\u00e3o da turbidez: um estudo de caso sobre S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-978e221 elementor-widget elementor-widget-heading\" data-id=\"978e221\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Materiais e M\u00e9todos<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-299f474 elementor-widget elementor-widget-text-editor\" data-id=\"299f474\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Preparou-se uma pr\u00e9-cultura de levedura de 100 mL em condi\u00e7\u00f5es est\u00e9reis, por meio da inocula\u00e7\u00e3o de col\u00f4nias <em>de S. cerevisiae<\/em> provenientes de uma cultura s\u00f3lida em placa de Petri, seguida de crescimento durante a noite. Simultaneamente, preparou-se um biorreator de 3 L (<a href=\"https:\/\/www.sartorius.com\/en\/products\/fermentation-bioreactors\/benchtop-bioreactors\/biostat-b?_gl=1*dzzxof*_up*MQ..&#038;gclid=CjwKCAjw-7OlBhB8EiwAnoOEkyL2kNBymV2yGELc9KSmyLWW_BODZo5jZrEa5-9mE9bDhvznnIe9FBoCDHsQAvD_BwE\">BIOSTAT B; Sartorius Stedim Biotech, G\u00f6ttingen, Alemanha<\/a>) contendo meio de cultura sint\u00e9tico est\u00e9ril [3] foi preparado para esteriliza\u00e7\u00e3o durante a noite em uma autoclave. O meio de cultura sint\u00e9tico consistia em v\u00e1rios sais inorg\u00e2nicos ((NH\u2084)\u2082SO\u2084, KH\u2082PO\u2084, MgSO\u2084), vitaminas, oligoelementos e glicose. Ap\u00f3s a esteriliza\u00e7\u00e3o, as sondas de pH e pO\u2082, bem como a bomba de alimenta\u00e7\u00e3o de glicose, foram calibradas seguindo as instru\u00e7\u00f5es fornecidas pelo software do BIOSTAT B (<strong>Figura 1<\/strong>).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8c2bea5 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"8c2bea5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1604\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" class=\"attachment-full size-full wp-image-29333\" alt=\"Yeast bioreactor in sterile conditions.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?w=1604&amp;ssl=1 1604w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=300%2C192&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1024%2C654&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=768%2C490&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1536%2C981&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1200%2C766&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=229%2C146&amp;ssl=1 229w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=50%2C32&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=117%2C75&amp;ssl=1 117w\" sizes=\"(max-width:767px) 700px, (max-width:1604px) 100vw, 1604px\" data-attachment-id=\"29333\" data-permalink=\"https:\/\/rheolution.com\/pt\/fig1_biostat-software_merged\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" data-orig-size=\"1604,1024\" data-comments-opened=\"1\" data-image-title=\"Yeast bioreactor\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1024%2C654&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f611ec3 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f611ec3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 1: <\/b>Configura\u00e7\u00e3o <b>do biorreator <\/b>para o cultivo de leveduras em condi\u00e7\u00f5es est\u00e9reis.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9aba0ca elementor-widget elementor-widget-text-editor\" data-id=\"9aba0ca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Para calibrar o <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, foram utilizados padr\u00f5es de formazina antes dos testes. A emiss\u00e3o e a recep\u00e7\u00e3o de luz foram ativadas utilizando cartuchos Emitt.805 (comprimento de onda de 850 nm) e cartuchos Receiv.ViS (comprimento de onda variando de 400 nm a 1.000 nm), respectivamente. Optamos por utilizar o cartucho de 850 nm, uma vez que o efeito da absor\u00e7\u00e3o de luz era menor. O espectrofot\u00f4metro (<a href=\"https:\/\/www.safas.com\/?SAFAS-UVmc1-spectrophotometer-the-price-and-use-of-a-single-beam-with-double-beam-quality&#038;lang=en\" target=\"_blank\" rel=\"noopener\">SAFAS UVmc, SAFAS, M\u00f4naco<\/a>) foi calibrado com um frasco de \u00e1gua destilada desionizada (ddH\u2082O) antes de cada medi\u00e7\u00e3o.  <\/p><p>As leveduras pr\u00e9-cultivadas foram semeadas no biorreator a uma densidade de 220 FTU (0,21 uOD). Posteriormente, o crescimento das leveduras foi monitorado por meio da coleta de al\u00edquotas a cada hora, durante 8 horas. As medi\u00e7\u00f5es de turbidez a 850 nm (expressas em FTU) e de absorv\u00e2ncia (expressas em uOD) foram realizadas em triplicata para cada ponto temporal (n = 3). Para as medi\u00e7\u00f5es espectrofotom\u00e9tricas, a al\u00edquota da cultura de leveduras foi dilu\u00edda na propor\u00e7\u00e3o de 1:100, enquanto que, para as medi\u00e7\u00f5es turbidim\u00e9tricas, n\u00e3o foi necess\u00e1ria dilui\u00e7\u00e3o, e as al\u00edquotas foram testadas diretamente. Os resultados s\u00e3o apresentados como m\u00e9dia \u00b1 desvio-padr\u00e3o.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2ad55a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"b2ad55a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">An\u00e1lise da din\u00e2mica de crescimento da levedura: medi\u00e7\u00f5es de turbidez versus absorv\u00e2ncia em culturas de S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aadb984 elementor-widget elementor-widget-heading\" data-id=\"aadb984\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resultados e Discuss\u00e3o<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99aa2a5 elementor-widget elementor-widget-text-editor\" data-id=\"99aa2a5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>A Figura 2<\/b> ilustra o padr\u00e3o de crescimento da levedura ao longo do tempo, apresentando um aumento exponencial not\u00e1vel tanto em unidades de turbidez (FTU) quanto em unidades de absorv\u00e2ncia (uOD), ap\u00f3s um curto per\u00edodo de lat\u00eancia de 2 horas. Durante a fase de lat\u00eancia, as c\u00e9lulas de levedura passam por um per\u00edodo de adapta\u00e7\u00e3o, ajustando-se gradualmente ao ambiente. \u00c0 medida que essa fase chega ao fim, a curva decola, demonstrando a prolifera\u00e7\u00e3o cont\u00ednua das c\u00e9lulas de levedura. Essa fase de crescimento exponencial indica a presen\u00e7a de condi\u00e7\u00f5es ideais para o crescimento da levedura, nas quais fatores como a disponibilidade de nutrientes e os est\u00edmulos ambientais promovem a divis\u00e3o celular ativa e a atividade metab\u00f3lica.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bd7401d elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"bd7401d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"2827\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29324\" alt=\"Turbidity trend of S. cerevisiae culture over 8h, 850nm vs. OD600nm comparison graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2827&amp;ssl=1 2827w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=300%2C170&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1024%2C580&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=768%2C435&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1536%2C870&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=2048%2C1160&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1200%2C680&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=258%2C146&amp;ssl=1 258w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=50%2C28&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=132%2C75&amp;ssl=1 132w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2440&amp;ssl=1 2440w\" sizes=\"(max-width:767px) 700px, (max-width:2827px) 100vw, 2827px\" data-attachment-id=\"29324\" data-permalink=\"https:\/\/rheolution.com\/pt\/fig2_cell-grow-evolution\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" data-orig-size=\"2827,1601\" data-comments-opened=\"1\" data-image-title=\"Turbidity trend of S. cerevisiae\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=1024%2C580&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7801f2e elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"7801f2e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: bold; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">Figura 2:  <\/span><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\">Configura\u00e7\u00e3o do biorreator para o cultivo de leveduras em condi\u00e7\u00f5es est\u00e9reis. Evolu\u00e7\u00e3o da turbidez de   <\/span><i style=\"font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">S. cerevisiae<\/i><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\"> cultura durante 8 horas e compara\u00e7\u00e3o entre a turbidez a 850 nm e a DO<\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\"><span style=\"font-size: 0.6em; vertical-align: sub;\">600 nm<\/span><\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-52474e0 elementor-widget elementor-widget-text-editor\" data-id=\"52474e0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>As medi\u00e7\u00f5es obtidas com <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\">o TURBIDI.T\u2122<\/a> para a turbidez (representadas no eixo Y \u00e0 esquerda) correspondem estreitamente aos valores de absorv\u00e2ncia obtidos com um espectrofot\u00f4metro padr\u00e3o (exibidos no eixo Y \u00e0 direita), j\u00e1 que ambas as curvas apresentam uma tend\u00eancia semelhante. Todos os desvios-padr\u00e3o foram muito baixos (barras de erro inclu\u00eddas no gr\u00e1fico), demonstrando a alta precis\u00e3o das medi\u00e7\u00f5es (variabilidade inferior a 1%). <\/p><p>Em conson\u00e2ncia com as observa\u00e7\u00f5es da <strong>Figura 2<\/strong>, h\u00e1 uma correla\u00e7\u00e3o linear positiva entre a turbidez e as medi\u00e7\u00f5es de OD600nm, com um impressionante coeficiente de determina\u00e7\u00e3o (R\u00b2) de 0,998 (<strong>Figura 3<\/strong>). Esse alto valor de R\u00b2 indica uma rela\u00e7\u00e3o forte e confi\u00e1vel entre a turbidez e a OD600 nm, sugerindo que as medi\u00e7\u00f5es de turbidez podem servir efetivamente como substituto das medi\u00e7\u00f5es baseadas na absorv\u00e2ncia no monitoramento do crescimento de leveduras. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-97440cd elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"97440cd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2001\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29326\" alt=\"FTU (850 nm) vs. uOD (600 nm) Correlation in Cell Culture Growth Graph.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?w=2001&amp;ssl=1 2001w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=300%2C240&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1024%2C819&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=768%2C614&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1536%2C1229&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1200%2C960&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=182%2C146&amp;ssl=1 182w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=50%2C40&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=94%2C75&amp;ssl=1 94w\" sizes=\"(max-width:767px) 700px, (max-width:2001px) 100vw, 2001px\" data-attachment-id=\"29326\" data-permalink=\"https:\/\/rheolution.com\/pt\/fig3_correlation-between-ftu-and-uod\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" data-orig-size=\"2001,1601\" data-comments-opened=\"1\" data-image-title=\"Correlation in Cell Culture Growth\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=1024%2C819&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ccd96b elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"1ccd96b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 3: Correla\u00e7\u00e3o <\/b>entre a FTU (850 nm) e a uOD (600 nm) na medi\u00e7\u00e3o do crescimento de culturas celulares.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8a7ac8d elementor-widget elementor-widget-text-editor\" data-id=\"8a7ac8d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>A estreita correspond\u00eancia entre as medi\u00e7\u00f5es de turbidez e de absorv\u00e2ncia, aliada ao alto coeficiente de correla\u00e7\u00e3o, destaca a efic\u00e1cia e a precis\u00e3o das medi\u00e7\u00f5es baseadas na turbidez realizadas com <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">o TURBIDI.T\u2122<\/a> para monitorar a din\u00e2mica de crescimento das leveduras.<\/p><p>Al\u00e9m disso, a capacidade de obter dados confi\u00e1veis sobre o crescimento sem a necessidade de dilui\u00e7\u00e3o da amostra \u2014 como exigido na an\u00e1lise espectrofotom\u00e9trica tradicional \u2014 refor\u00e7a ainda mais as vantagens oferecidas pela abordagem baseada na turbidez. De modo geral, esses resultados demonstram o potencial do <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> como uma ferramenta valiosa para o monitoramento preciso e eficiente do crescimento de leveduras em bioprocessos, contribuindo para avan\u00e7os na pesquisa e nas aplica\u00e7\u00f5es biotecnol\u00f3gicas.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19ccca4 elementor-widget elementor-widget-heading\" data-id=\"19ccca4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Conclus\u00f5es e Perspectivas<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a93e4fa elementor-widget elementor-widget-text-editor\" data-id=\"a93e4fa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>O sistema <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> demonstrou ser uma ferramenta poderosa e precisa para monitorar o crescimento e a cin\u00e9tica de culturas de leveduras.  <\/li><li>A compara\u00e7\u00e3o entre a turbidez e o OD600nm revela uma forte rela\u00e7\u00e3o linear, estabelecendo a turbidez como uma alternativa precisa \u00e0s medi\u00e7\u00f5es baseadas na absorv\u00e2ncia. Isso destaca a efic\u00e1cia da turbidez na avalia\u00e7\u00e3o precisa da din\u00e2mica de crescimento da levedura. <\/li><li><a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">O TURBIDI.T\u2122<\/a> apresenta diversas vantagens em compara\u00e7\u00e3o com a an\u00e1lise por espectrofot\u00f4metro. A elimina\u00e7\u00e3o da dilui\u00e7\u00e3o da amostra, devido \u00e0 ampla faixa de valores de turbidez lidos pelo instrumento <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, reduz os erros de medi\u00e7\u00e3o e economiza um tempo valioso. Al\u00e9m disso, o tablet port\u00e1til facilita a coleta e o armazenamento de dados, aumentando a conveni\u00eancia e a acessibilidade.    <\/li><li>A versatilidade do sistema <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> permite a utiliza\u00e7\u00e3o de diferentes frascos para medi\u00e7\u00f5es em diversas configura\u00e7\u00f5es experimentais.<\/li><li>A escalabilidade do sistema <a href=\"https:\/\/rheolution.com\/pt\/produtos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> aumenta ainda mais seu valor. A conex\u00e3o de v\u00e1rias unidades a um \u00fanico tablet operacional melhora a efici\u00eancia dos testes, permitindo medi\u00e7\u00f5es simult\u00e2neas em experimentos de maior escala. <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d297cde elementor-align-center elementor-widget elementor-widget-button\" data-id=\"d297cde\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/wp-content\/uploads\/Microbiology_turbidity-1.pdf\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Acesse a Nota do Aplicativo na \u00edntegra (PDF) <\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee666c7 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"ee666c7\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper 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obter um or\u00e7amento ou para saber mais<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69d0943 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"69d0943\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/pt\/contato\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Entre em contato conosco para mais informa\u00e7\u00f5es<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-469fdb5 elementor-widget elementor-widget-heading\" data-id=\"469fdb5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Refer\u00eancias<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-685d6db elementor-widget elementor-widget-text-editor\" data-id=\"685d6db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>[1]  <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S095816692100152X\" target=\"_blank\" rel=\"noopener\">Reardon, K. F. (2021). Practical monitoring technologies for cells and substrates in biomanufacturing. Current Opinion in Biotechnology, 71, 225-230.  <\/a><\/p><p>[2]  <a href=\"https:\/\/link.springer.com\/article\/10.1186\/s12934-021-01617-z\" target=\"_blank\" rel=\"noopener\">Madhavan, A., Arun, K. B., Sindhu, R., Krishnamoorthy, J., Reshmy, R., Sirohi, R., &#8230; &#038; Binod, P. (2021). Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up. Microbial cell factories, 20(1), 124.  <\/a><\/p><p>[3]<a href=\"https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/\" target=\"_blank\" rel=\"noopener\">Tankeshwar Acharya (2021). Bacterial Culture Media: Classification, Types, Uses. Retrieved from https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/.  <\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec9a286 elementor-widget elementor-widget-heading\" data-id=\"ec9a286\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Posts Relacionados<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca12d3f premium-blog-cta-full-yes premium-floating-effects-yes premium-disable-fe-yes elementor-widget__width-initial premium-blog-align-left elementor-widget elementor-widget-premium-addon-blog\" data-id=\"ca12d3f\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;force_height&quot;:&quot;true&quot;,&quot;premium_blog_grid&quot;:&quot;yes&quot;,&quot;premium_blog_layout&quot;:&quot;even&quot;,&quot;premium_blog_columns_number&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_tablet&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_mobile&quot;:&quot;100%&quot;,&quot;scroll_to_offset&quot;:&quot;yes&quot;,&quot;premium_fe_trigger&quot;:&quot;load&quot;,&quot;premium_fe_direction&quot;:&quot;alternate&quot;,&quot;premium_fe_loop&quot;:&quot;default&quot;,&quot;premium_fe_easing&quot;:&quot;easeInOutSine&quot;}\" data-widget_type=\"premium-addon-blog.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t\n\n\t\t\t\t<div class=\"premium-blog-wrap  premium-blog-even\" data-page=\"70932\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65520\" alt=\"Blog Post Image: Methods to Quantify Cell Concentration Using TURBIDI.T\u2122 Technology.\" data-attachment-id=\"65520\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/quantificar-a-concentracao-celular-com-o-metodo-turbidimetrico\/attachment\/quantifying-cell-concentration-in-culture-media-with-turbidi-t-header-3-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Methods to Quantify Cell Concentration\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1024%2C384&amp;ssl=1\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/quantificar-a-concentracao-celular-com-o-metodo-turbidimetrico\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Como quantificar a concentra\u00e7\u00e3o celular com o TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/quantificar-a-concentracao-celular-com-o-metodo-turbidimetrico\/\" target=\"_blank\">\n\t\t\t\tComo quantificar a concentra\u00e7\u00e3o celular com o TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">A crescente demanda por m\u00e9todos mais r\u00e1pidos de quantifica\u00e7\u00e3o celular vai al\u00e9m das c\u00e2maras de contagem tradicionais, levando ao desenvolvimento de solu\u00e7\u00f5es inovadoras que facilitam a contagem precisa e, ao mesmo tempo, reduzem o tempo necess\u00e1rio. A turbidez, que reflete a opacidade da solu\u00e7\u00e3o na presen\u00e7a de part\u00edculas que espalham a luz, surge como um meio eficaz para acelerar a quantifica\u00e7\u00e3o celular.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/quantificar-a-concentracao-celular-com-o-metodo-turbidimetrico\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65595\" alt=\"Measuring liquid particle size with TURBIDI.T\u2122.\" data-attachment-id=\"65595\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/medir-o-tamanho-das-particulas-em-uma-amostra-liquida\/attachment\/web_banner_size-4-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Measuring liquid particle\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1024%2C384&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?w=1200&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=300%2C113&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=1024%2C384&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=768%2C288&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=260%2C98&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=50%2C19&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=150%2C56&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/medir-o-tamanho-das-particulas-em-uma-amostra-liquida\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Como medir o tamanho das part\u00edculas em uma amostra l\u00edquida com o TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/medir-o-tamanho-das-particulas-em-uma-amostra-liquida\/\" target=\"_blank\">\n\t\t\t\tComo medir o tamanho das part\u00edculas em uma amostra l\u00edquida com o TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">A determina\u00e7\u00e3o do tamanho das part\u00edculas \u00e9 importante em diversas \u00e1reas, como as ci\u00eancias da vida (nanomedicina, administra\u00e7\u00e3o de medicamentos, engenharia de tecidos, bioan\u00e1lise), qu\u00edmica, ci\u00eancias ambientais e ind\u00fastrias. A turbidimetria \u00e9 um m\u00e9todo r\u00e1pido e n\u00e3o destrutivo para estimar o tamanho das part\u00edculas, seguindo um procedimento f\u00e1cil de executar.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/medir-o-tamanho-das-particulas-em-uma-amostra-liquida\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>No campo da bioengenharia e da biotecnologia, as c\u00e9lulas atuam como catalisadores na produ\u00e7\u00e3o de diversos produtos valiosos, tais como biocombust\u00edveis, produtos farmac\u00eauticos, anticorpos, enzimas industriais e produtos qu\u00edmicos de origem biol\u00f3gica [1]. Nesse contexto, a levedura, um microrganismo amplamente utilizado, desempenha um papel fundamental. O monitoramento do crescimento da levedura \u00e9 de extrema import\u00e2ncia para garantir condi\u00e7\u00f5es ideais e produtividade nos processos biotecnol\u00f3gicos [2]. Tradicionalmente, a an\u00e1lise espectrofotom\u00e9trica tem sido utilizada para avaliar a densidade \u00f3ptica a 600 nm (DO600nm), medindo a absor\u00e7\u00e3o de luz pela levedura&#8230;   <\/p>\n","protected":false},"author":195401338,"featured_media":70933,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[286,319,314],"tags":[123,148,124,121,137],"class_list":["post-70932","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-notas-de-aplicacao","category-turbidi-t-life-sciences","category-turbidit-agrifood","tag-agri-food","tag-comparison-of-technologies","tag-life-sciences","tag-microbiology","tag-agri-food-turbidity"],"yoast_head":"<!-- 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