{"id":70913,"date":"2026-02-03T16:34:19","date_gmt":"2026-02-03T21:34:19","guid":{"rendered":"https:\/\/rheolution.com\/?p=70913"},"modified":"2026-10-08T15:59:18","modified_gmt":"2026-10-08T19:59:18","slug":"reticulacao-da-gelatina-com-transglutaminase-microbiana","status":"publish","type":"post","link":"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/reticulacao-da-gelatina-com-transglutaminase-microbiana\/","title":{"rendered":"Reticula\u00e7\u00e3o da gelatina com transglutaminase microbiana"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70913\" class=\"elementor elementor-70913 elementor-63479\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8fd4dcf e-flex e-con-boxed e-con e-parent\" data-id=\"8fd4dcf\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8c770e2 elementor-widget elementor-widget-heading\" data-id=\"8c770e2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota de Aplica\u00e7\u00e3o | ElastoSens\u2122 Bio<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-760ae40 elementor-widget elementor-widget-heading\" data-id=\"760ae40\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Reticula\u00e7\u00e3o da gelatina com transglutaminase microbiana: caracteriza\u00e7\u00e3o viscoel\u00e1stica\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-16057f6 elementor-widget elementor-widget-text-editor\" data-id=\"16057f6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>por <a href=\"https:\/\/www.linkedin.com\/in\/mayasalame\/\">Maya Salame<\/a> e <a href=\"https:\/\/www.linkedin.com\/in\/dimitria-camas%C3%A3o-049002a2\/\">Dimitria Camasao<\/a><\/p><p>Cientistas de Aplica\u00e7\u00f5es, <a href=\"https:\/\/www.linkedin.com\/company\/1168758\" target=\"_blank\" rel=\"noopener\">Rheolution Inc.<\/a><\/p><p>Em colabora\u00e7\u00e3o com  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-414c0c0 e-grid e-con-full e-con e-child\" data-id=\"414c0c0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4585270 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"4585270\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"350\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1024%2C350&amp;ssl=1\" class=\"attachment-large size-large wp-image-63676\" alt=\"Zedira\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?w=1805&amp;ssl=1 1805w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=300%2C103&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=1024%2C350&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=768%2C263&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=1536%2C525&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=150%2C51&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=700%2C239&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63676\" data-permalink=\"https:\/\/rheolution.com\/pt\/zedira-logo\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1805%2C617&amp;ssl=1\" data-orig-size=\"1805,617\" data-comments-opened=\"1\" data-image-meta=\"{&quot;credit&quot;:&quot;bott&quot;,&quot;created_timestamp&quot;:&quot;1466443687&quot;}\" data-image-title=\"Zedira Logo\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1024%2C350&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-252ef73 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"252ef73\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"318\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=1024%2C318&amp;ssl=1\" class=\"attachment-large size-large wp-image-63668\" alt=\"Rousselot Biomedical\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=4126&amp;ssl=1 4126w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=300%2C93&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=1024%2C318&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=768%2C238&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=1536%2C477&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=2048%2C635&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=150%2C47&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=700%2C217&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63668\" data-permalink=\"https:\/\/rheolution.com\/pt\/logo_rousselot_biomedical_tagline-1_rgb\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=4126%2C1280&amp;ssl=1\" data-orig-size=\"4126,1280\" data-comments-opened=\"1\" data-image-title=\"Logo_Rousselot_Biomedical_Tagline-1_RGB\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=1024%2C318&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-97ac8de e-flex e-con-boxed e-con e-parent\" data-id=\"97ac8de\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5fc8fde elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5fc8fde\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resumo<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d61237b elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"d61237b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Foi estabelecido um protocolo de refer\u00eancia para a reticula\u00e7\u00e3o enzim\u00e1tica da X-Pure Gelatin\u00ae utilizando a transglutaminase microbiana Andracon\u00ae, com monitoramento mec\u00e2nico em tempo real por meio do ElastoSens\u2122 Bio.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">O aumento da concentra\u00e7\u00e3o de gelatina aumentou significativamente a rigidez final do gel (G\u2032) e reduziu o tempo de gelifica\u00e7\u00e3o.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">O aumento da concentra\u00e7\u00e3o de MTG elevou a resist\u00eancia mec\u00e2nica final e acelerou significativamente a gelifica\u00e7\u00e3o e a estabiliza\u00e7\u00e3o da rede.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">A temperatura de reticula\u00e7\u00e3o (37 \u00b0C vs. 45 \u00b0C) n\u00e3o teve efeito significativo nas propriedades viscoel\u00e1sticas finais, confirmando a compatibilidade com as condi\u00e7\u00f5es fisiol\u00f3gicas.<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">O ElastoSens\u2122 Bio demonstrou ser uma ferramenta valiosa para o estabelecimento de protocolos de refer\u00eancia para a reticula\u00e7\u00e3o enzim\u00e1tica da gelatina e para o estudo da influ\u00eancia dos par\u00e2metros de formula\u00e7\u00e3o e de processo na cin\u00e9tica de gelifica\u00e7\u00e3o e nas propriedades mec\u00e2nicas.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-82969b3 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"82969b3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Introdu\u00e7\u00e3o<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5703c6a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5703c6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Gelatina: um biopol\u00edmero vers\u00e1til<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38524ee elementor-widget elementor-widget-text-editor\" data-id=\"38524ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">A gelatina \u00e9 um biopol\u00edmero amplamente utilizado em biomateriais, pois pode ser processada em \u00e1gua, \u00e9 biocompat\u00edvel e \u00e9 capaz de formar redes macias e hidratadas. No entanto, os g\u00e9is f\u00edsicos de gelatina podem enfraquecer ou derreter em temperaturas pr\u00f3ximas \u00e0s fisiol\u00f3gicas; por isso, a reticula\u00e7\u00e3o covalente \u00e9 comumente utilizada para melhorar a estabilidade t\u00e9rmica e a integridade mec\u00e2nica.   <\/span><a href=\"https:\/\/www.darlingii.com\/rousselot\/brands\/xpure\"><span style=\"font-weight: 400;\">X-Pure Gelatin\u00ae<\/span><\/a><span style=\"font-weight: 400;\"> \u00e9 uma gelatina de alta qualidade, de grau farmac\u00eautico, caracterizada por rigorosos padr\u00f5es de pureza e desempenho consistente.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-29574d5 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"29574d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Reticula\u00e7\u00e3o de pol\u00edmeros com a transglutaminase microbiana (MTG) Andracon\u00ae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2e827a1 elementor-widget elementor-widget-text-editor\" data-id=\"2e827a1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">O bio-reticulante transglutaminase microbiana catalisa a forma\u00e7\u00e3o de liga\u00e7\u00f5es covalentes entre res\u00edduos de glutamina e lisina na gelatina, permitindo a forma\u00e7\u00e3o controlada de uma rede em condi\u00e7\u00f5es aquosas suaves. <\/span><a href=\"https:\/\/zedira.com\/Microbial-Transglutaminase\"><span style=\"font-weight: 400;\">O Andracon\u00ae<\/span><\/a><span style=\"font-weight: 400;\"> \u00e9 uma transglutaminase microbiana de alta qualidade destinada \u00e0 pesquisa pr\u00e9-cl\u00ednica e at\u00e9 aplica\u00e7\u00f5es cl\u00ednicas.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-885800d elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"885800d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Estudo de caso: Caracteriza\u00e7\u00e3o mec\u00e2nica da reticula\u00e7\u00e3o da gelatina por transglutaminase utilizando o ElastoSens\u2122 Bio<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b87c673 elementor-widget elementor-widget-text-editor\" data-id=\"b87c673\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Neste trabalho, foi definido um protocolo de reticula\u00e7\u00e3o de refer\u00eancia utilizando gelatina X-Pure Gelatin\u00ae de pele de porco tratada com \u00e1cido e Andracon\u00ae MTG, e a gelifica\u00e7\u00e3o foi monitorada de forma n\u00e3o destrutiva com o ElastoSens\u2122 Bio. O instrumento foi utilizado para quantificar a evolu\u00e7\u00e3o viscoel\u00e1stica em fun\u00e7\u00e3o do tempo das formula\u00e7\u00f5es de gelatina\u2013MTG durante a reticula\u00e7\u00e3o. O m\u00f3dulo de armazenamento ao cisalhamento (G\u2032) descreve o componente el\u00e1stico do material, respons\u00e1vel pelo armazenamento de energia, e \u00e9 comumente interpretado como um indicador da rigidez do gel \u00e0 medida que a rede se forma. O m\u00f3dulo de perda ao cisalhamento (G\u2033) representa o componente viscoso, respons\u00e1vel pela dissipa\u00e7\u00e3o de energia, e reflete a contribui\u00e7\u00e3o de car\u00e1ter fluido durante a reticula\u00e7\u00e3o. O fator de amortecimento (tan \u03b4 = G\u2033\/G\u2032) fornece uma medida adimensional do equil\u00edbrio entre as respostas viscosas e el\u00e1sticas e varia de 0 (comportamento semelhante ao de um l\u00edquido, com predomin\u00e2ncia de G\u2033) a 1 (comportamento semelhante ao de um s\u00f3lido, com predomin\u00e2ncia de G\u2032). O ponto de gelifica\u00e7\u00e3o (transi\u00e7\u00e3o sol-gel) foi definido como o momento em que G\u2032 = G\u2033; o tempo at\u00e9 o ponto de gelifica\u00e7\u00e3o foi relatado como o tempo decorrido desde o in\u00edcio da medi\u00e7\u00e3o at\u00e9 esse cruzamento. O monitoramento cont\u00ednuo gerou curvas cin\u00e9ticas completas de gelifica\u00e7\u00e3o, possibilitando (i) identificar quando o sinal atingiu uma regi\u00e3o de plat\u00f4 e (ii) calcular os valores finais estabilizados de G\u2032, G\u2033 e tan \u03b4 por meio da m\u00e9dia nessa regi\u00e3o de plat\u00f4. As mesmas curvas cin\u00e9ticas tamb\u00e9m foram utilizadas para determinar o tempo at\u00e9 a plat\u00f4, definido como o tempo total necess\u00e1rio para que as propriedades viscoel\u00e1sticas se estabilizassem (G\u2032 atingiu 90% de seu valor final (plat\u00f4)).         <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df34a6d elementor-widget-mobile__width-initial elementor-widget elementor-widget-image\" data-id=\"df34a6d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"968\" height=\"544\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" class=\"attachment-large size-large wp-image-50363\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?w=968&amp;ssl=1 968w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=300%2C169&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=768%2C432&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=133%2C75&amp;ssl=1 133w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=480%2C270&amp;ssl=1 480w\" sizes=\"(max-width:767px) 700px, (max-width:968px) 100vw, 968px\" data-attachment-id=\"50363\" data-permalink=\"https:\/\/rheolution.com\/pt\/elastosens2-4-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" data-orig-size=\"968,544\" data-comments-opened=\"1\" data-image-title=\"elastosens2\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ed23fc9 elementor-widget elementor-widget-heading\" data-id=\"ed23fc9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Materiais e m\u00e9todos<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d118051 elementor-widget elementor-widget-text-editor\" data-id=\"d118051\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Gelatina de pele de porco \u00e1cida (<\/span><a href=\"https:\/\/www.darlingii.com\/en\/rousselot\/brands\/xpure\"><span style=\"font-weight: 400;\">X-Pure Gelatin\u00ae, Rousselot<\/span><\/a><span style=\"font-weight: 400;\">; HBHV, ~300 Bloom) foi reticulada com transglutaminase microbiana (<\/span><a href=\"https:\/\/zedira.com\/Microbial-Transglutaminase\"><span style=\"font-weight: 400;\">Andracon\u00ae, Zedira<\/span><\/a><span style=\"font-weight: 400;\">) e monitorada em tempo real com o ElastoSens\u2122 Bio (<\/span><a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\"><span style=\"font-weight: 400;\">Rheolution<\/span><\/a><span style=\"font-weight: 400;\">). <\/span> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4499b74 elementor-widget elementor-widget-heading\" data-id=\"4499b74\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>Condi\u00e7\u00f5es testadas:<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d014f83 elementor-widget elementor-widget-text-editor\" data-id=\"d014f83\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Concentra\u00e7\u00e3o de gelatina: 5, 10, 15% em peso  <\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Carga de MTG: 15 e 30 U g\u207b\u00b9 de gelatina<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Temperatura: 37 \u00b0C e 45 \u00b0C<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Volume da amostra do ElastoSens: 2,0 mL<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Dura\u00e7\u00e3o da medi\u00e7\u00e3o: 6 h<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Repeti\u00e7\u00f5es: n = 3 por condi\u00e7\u00e3o<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23a931e elementor-widget elementor-widget-text-editor\" data-id=\"23a931e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">As solu\u00e7\u00f5es-m\u00e3e de gelatina foram preparadas a 5% em peso (50 mg mL\u207b\u00b9), 10% em peso (100 mg mL\u207b\u00b9) e 15% em peso (150 mg mL\u207b\u00b9) por meio da dissolu\u00e7\u00e3o da gelatina em PBS. As solu\u00e7\u00f5es foram incubadas em banho-maria a 60 \u00b0C por 60 minutos sob agita\u00e7\u00e3o magn\u00e9tica cont\u00ednua para garantir a dissolu\u00e7\u00e3o completa. Paralelamente, a solu\u00e7\u00e3o-m\u00e3e da enzima Andracon\u00ae (atividade total de 116 U) foi preparada em \u00e1gua desionizada como solu\u00e7\u00e3o de trabalho a 341,2 U mL\u207b\u00b9 e equilibrada a 37 \u00b0C por 5 minutos antes do uso (armazenada a 4 \u00b0C quando n\u00e3o estiver em uso).  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58a09c6 elementor-widget elementor-widget-heading\" data-id=\"58a09c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>Carregamento da amostra e medi\u00e7\u00e3o com o ElastoSens\u2122 Bio:<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b9dfced elementor-widget elementor-widget-text-editor\" data-id=\"b9dfced\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">A reticula\u00e7\u00e3o foi iniciada pela adi\u00e7\u00e3o do volume calculado da solu\u00e7\u00e3o-m\u00e3e de MTG \u00e0 solu\u00e7\u00e3o de gelatina, a fim de atingir o valor alvo de U g\u207b\u00b9 (Tabela 1).<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">As amostras foram misturadas delicadamente com uma barra de agita\u00e7\u00e3o magn\u00e9tica por alguns segundos para homogeneiza\u00e7\u00e3o.<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">O instrumento foi pr\u00e9-equilibrado \u00e0 temperatura desejada (37 \u00b0C ou 45 \u00b0C). Imediatamente ap\u00f3s a mistura, 2,0 mL da mistura de rea\u00e7\u00e3o foram transferidos para o porta-amostra macro e a medi\u00e7\u00e3o foi realizada de forma cont\u00ednua por 6 h. <\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-294dbc1 elementor-widget elementor-widget-text-editor\" data-id=\"294dbc1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Tabela 1:<\/b><span style=\"font-weight: 400;\"> Volume de transglutaminase microbiana (MTG) necess\u00e1rio para a reticula\u00e7\u00e3o da gelatina, calculado para um volume de prepara\u00e7\u00e3o de 2,5 mL, utilizando uma concentra\u00e7\u00e3o de solu\u00e7\u00e3o-m\u00e3e de MTG de 341,2 U mL\u207b\u00b9.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5929e04 eael-dt-th-align-center eael-table-align-center elementor-widget elementor-widget-eael-data-table\" data-id=\"5929e04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-data-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"eael-data-table-wrap\" data-table_id=\"5929e04\" id=\"eael-data-table-wrapper-5929e04\" data-custom_responsive=\"false\">\n\t\t\t<table class=\"tablesorter eael-data-table center\" id=\"eael-data-table-5929e04\">\n\t\t\t    <thead>\n\t\t\t        <tr class=\"table-header\">\n\t\t\t\t\t\t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Concentra\u00e7\u00e3o de gelatina [% em peso]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Concentra\u00e7\u00e3o de gelatina [g L\u207b\u00b9]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Gelatina total em 2,5 mL [g]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Dose-alvo de MTG [U g\u207b\u00b9]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Atividade total de MTG em 2,5 mL [U]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Volume da amostra de MTG [\u00b5L]<\/span><\/th>\n\t\t\t        \t\t\t\t        <\/tr>\n\t\t\t    <\/thead>\n\t\t\t  \t<tbody>\n\t\t\t\t\t\t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t50\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.125\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t3.75\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t10\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.250\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t7.50\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t22\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t15\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t150\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.375\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11.25\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t33\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t10\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.250\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t15\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t3.75\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t    <\/tbody>\n\t\t\t<\/table>\n\t\t<\/div>\n\t  \t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d907f30 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"d907f30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"889\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1024%2C889&amp;ssl=1\" class=\"attachment-large size-large wp-image-63545\" alt=\"Sample Holder with Sample\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?w=1240&amp;ssl=1 1240w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=300%2C260&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=1024%2C889&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=768%2C666&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=86%2C75&amp;ssl=1 86w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=700%2C607&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63545\" data-permalink=\"https:\/\/rheolution.com\/pt\/sample-holder-with-sample\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1240%2C1076&amp;ssl=1\" data-orig-size=\"1240,1076\" data-comments-opened=\"1\" data-image-title=\"Sample Holder with Sample\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1024%2C889&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-39e927a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"39e927a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 1.<\/b><span style=\"font-weight: 400;\">  Hidrog\u00e9is de gelatina representativos (n = 3) ap\u00f3s a remo\u00e7\u00e3o do suporte de amostras de grande porte, ap\u00f3s a conclus\u00e3o do experimento de gelifica\u00e7\u00e3o.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8cc0f04 elementor-widget elementor-widget-heading\" data-id=\"8cc0f04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>An\u00e1lise estat\u00edstica:<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-54513af elementor-widget elementor-widget-text-editor\" data-id=\"54513af\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Os dados foram analisados por ANOVA unidirecional (Brown\u2013Forsythe\/Welch para lidar com vari\u00e2ncias desiguais). As compara\u00e7\u00f5es pareadas planejadas utilizaram testes t de Welch n\u00e3o pareados (sem ajuste para compara\u00e7\u00f5es m\u00faltiplas). Os dados s\u00e3o apresentados como m\u00e9dia \u00b1 DP;    <\/span><i><span style=\"font-weight: 400;\">p<\/span><\/i><span style=\"font-weight: 400;\">&lt; 0,05.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3ded5ea elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"3ded5ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Comportamento viscoel\u00e1stico durante a reticula\u00e7\u00e3o da gelatina: resultados e discuss\u00e3o<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca90a6e elementor-widget elementor-widget-text-editor\" data-id=\"ca90a6e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Este estudo foi elaborado para avaliar a reticula\u00e7\u00e3o enzim\u00e1tica da gelatina por meio da MTG e para investigar a influ\u00eancia de par\u00e2metros-chave no comportamento de gelifica\u00e7\u00e3o e nas propriedades mec\u00e2nicas. Especificamente, foram examinados os efeitos da concentra\u00e7\u00e3o de gelatina, da concentra\u00e7\u00e3o de transglutaminase e da temperatura de reticula\u00e7\u00e3o. Na maioria dos experimentos, foi utilizada uma temperatura de reticula\u00e7\u00e3o de 45 \u00b0C para evitar a gelifica\u00e7\u00e3o t\u00e9rmica da gelatina, garantindo que a gelifica\u00e7\u00e3o fosse devida exclusivamente \u00e0 reticula\u00e7\u00e3o enzim\u00e1tica. Essa temperatura tamb\u00e9m se encontra dentro de uma faixa favor\u00e1vel para uma atividade enzim\u00e1tica elevada e est\u00e1vel.   <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2f0036 elementor-widget elementor-widget-heading\" data-id=\"b2f0036\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Efeito da concentra\u00e7\u00e3o de gelatina na gelifica\u00e7\u00e3o e nas propriedades mec\u00e2nicas<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-213f704 elementor-widget elementor-widget-text-editor\" data-id=\"213f704\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">A Figura 2 mostra a evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento de cisalhamento (G\u2032) em fun\u00e7\u00e3o do tempo durante a reticula\u00e7\u00e3o da gelatina com transglutaminase microbiana. A linha em negrito representa a m\u00e9dia das triplicatas, enquanto as linhas tracejadas representam o desvio padr\u00e3o. Para todas as amostras, G\u2032 iniciou em valores baixos e apresentou um forte aumento ap\u00f3s o ponto de transi\u00e7\u00e3o l\u00edquido-gel, chegando eventualmente a um patamar est\u00e1vel.    <\/span><\/p><p><span style=\"font-weight: 400;\">A Figura 3 mostra os valores m\u00e9dios de G\u2032 e G\u2033 durante a \u00faltima hora do teste. Como esperado, o valor final de G\u2032 aumentou com o aumento da concentra\u00e7\u00e3o de gelatina (***p &lt; 0,001), indicando que a rede formada \u00e9 mais resistente devido \u00e0 presen\u00e7a de um n\u00famero maior de mol\u00e9culas de gelatina. G\u2019\u2019 tamb\u00e9m apresentou uma tend\u00eancia semelhante; no entanto, n\u00e3o foi encontrada diferen\u00e7a estat\u00edstica neste estudo. O fator de amortecimento (tan \u03b4 = G\u2033\/G\u2032) foi semelhante entre as condi\u00e7\u00f5es de 15% em peso e 10% em peso e ligeiramente maior para a condi\u00e7\u00e3o de 5% em peso, indicando uma maior predomin\u00e2ncia viscosa nesta \u00faltima em compara\u00e7\u00e3o com as outras.   <\/span><\/p><p><span style=\"font-weight: 400;\">A Figura 4 mostra o tempo necess\u00e1rio para atingir o ponto de transi\u00e7\u00e3o l\u00edquido-gel (G\u2019 = G\u2019\u2019, \u00e0 esquerda) e o tempo total necess\u00e1rio para atingir o plat\u00f4 (\u00e0 direita). Como esperado, o tempo necess\u00e1rio para atingir a transi\u00e7\u00e3o l\u00edquido-gel diminuiu com o aumento da concentra\u00e7\u00e3o de gelatina e foi de aproximadamente 0,5, 10 e 14 minutos para as amostras de 15% em peso, 10% em peso e 5% em peso, respectivamente. Esse comportamento \u00e9 atribu\u00eddo \u00e0 maior disponibilidade de cadeias polim\u00e9ricas de gelatina em concentra\u00e7\u00f5es mais elevadas, o que promove uma forma\u00e7\u00e3o mais r\u00e1pida da rede e acelera a transi\u00e7\u00e3o l\u00edquido-gel. Curiosamente, embora cada condi\u00e7\u00e3o tenha exigido um tempo diferente para atingir o ponto de transi\u00e7\u00e3o, todas levaram um tempo semelhante para atingir o patamar, sugerindo que, uma vez iniciada a gelifica\u00e7\u00e3o, a cin\u00e9tica de reticula\u00e7\u00e3o subsequente \u00e9 governada principalmente pela atividade enzim\u00e1tica, e n\u00e3o pela concentra\u00e7\u00e3o de gelatina.   <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a19c2ed elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a19c2ed\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"640\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1024%2C640&amp;ssl=1\" class=\"attachment-large size-large wp-image-63537\" alt=\"15%, 10%, 5% compare [gelatin] - G&apos; kinetics\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?w=1542&amp;ssl=1 1542w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=300%2C187&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=1024%2C640&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=768%2C480&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=1536%2C959&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=120%2C75&amp;ssl=1 120w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=960%2C600&amp;ssl=1 960w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=700%2C437&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63537\" data-permalink=\"https:\/\/rheolution.com\/pt\/15-10-5-compare-gelatin-g-kinetics\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1542%2C963&amp;ssl=1\" data-orig-size=\"1542,963\" data-comments-opened=\"1\" data-image-title=\"15%, 10%, 5% compare [gelatin] &amp;#8211; G&amp;#8217; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1024%2C640&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6327b34 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"6327b34\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 2.<\/b><span style=\"font-weight: 400;\">  Evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento ao cisalhamento (G\u2032) durante a gelifica\u00e7\u00e3o de hidrog\u00e9is de gelatina a 15%, 10% e 5% em peso, reticulados a 45 \u00b0C com 30 U g\u207b\u00b9 de transglutaminase.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a08afe4 e-grid e-con-full e-con e-child\" data-id=\"a08afe4\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0cfd1bb elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"0cfd1bb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"740\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=740%2C1024&amp;ssl=1\" class=\"attachment-large size-large wp-image-63497\" alt=\"gelatin effect - G&apos; Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?w=750&amp;ssl=1 750w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=217%2C300&amp;ssl=1 217w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=740%2C1024&amp;ssl=1 740w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=54%2C75&amp;ssl=1 54w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=700%2C969&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 740px\" data-attachment-id=\"63497\" data-permalink=\"https:\/\/rheolution.com\/pt\/gelatin-effect-g-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=750%2C1038&amp;ssl=1\" data-orig-size=\"750,1038\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; G&amp;#8217; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=740%2C1024&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-683a272 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"683a272\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"667\" height=\"1013\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" class=\"attachment-large size-large wp-image-63521\" alt=\"gelatin effect- G&apos;&apos; Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?w=667&amp;ssl=1 667w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?resize=198%2C300&amp;ssl=1 198w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?resize=49%2C75&amp;ssl=1 49w\" sizes=\"(max-width:767px) 667px, 667px\" data-attachment-id=\"63521\" data-permalink=\"https:\/\/rheolution.com\/pt\/gelatin-effect-g-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" data-orig-size=\"667,1013\" data-comments-opened=\"1\" data-image-title=\"gelatin effect- G&amp;#8221; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1950aba elementor-widget elementor-widget-image\" data-id=\"1950aba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"688\" height=\"942\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" class=\"attachment-large size-large wp-image-63529\" alt=\"gelatin effect- TanD Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?w=688&amp;ssl=1 688w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?resize=219%2C300&amp;ssl=1 219w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?resize=55%2C75&amp;ssl=1 55w\" sizes=\"(max-width:767px) 688px, 688px\" data-attachment-id=\"63529\" data-permalink=\"https:\/\/rheolution.com\/pt\/gelatin-effect-tand-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" data-orig-size=\"688,942\" data-comments-opened=\"1\" data-image-title=\"gelatin effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f383e58 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f383e58\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 3.<\/b><span style=\"font-weight: 400;\">  Propriedades viscoel\u00e1sticas finais dos hidrog\u00e9is de gelatina reticulados com transglutaminase a 45 \u00b0C. O m\u00f3dulo de armazenamento ao cisalhamento (G\u2032, \u00e0 esquerda), o m\u00f3dulo de perda ao cisalhamento (G\u2033, no meio) e o fator de amortecimento (tan \u03b4, \u00e0 direita) foram calculados a partir da regi\u00e3o de plat\u00f4 (5\u20136 h) das curvas de gelifica\u00e7\u00e3o para hidrog\u00e9is de gelatina a 15, 10, e 5% em peso de hidrog\u00e9is de gelatina reticulados com 30 U g\u207b\u00b9 de transglutaminase. As barras mostram a m\u00e9dia \u00b1 DP, com pontos de dados individuais sobrepostos. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como *p &lt; 0,05, **p &lt; 0,01, ****p &lt; 0,0001; ns, n\u00e3o significativo.   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-ee8f627 e-grid e-con-full e-con e-child\" data-id=\"ee8f627\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0cfcaef elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"0cfcaef\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"703\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=703%2C1024&amp;ssl=1\" class=\"attachment-large size-large wp-image-63505\" alt=\"gelatin effect - sol-gel transition\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?w=713&amp;ssl=1 713w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=206%2C300&amp;ssl=1 206w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=703%2C1024&amp;ssl=1 703w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=52%2C75&amp;ssl=1 52w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=700%2C1019&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 703px\" data-attachment-id=\"63505\" data-permalink=\"https:\/\/rheolution.com\/pt\/gelatin-effect-sol-gel-transition\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=713%2C1038&amp;ssl=1\" data-orig-size=\"713,1038\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=703%2C1024&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d66ae20 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"d66ae20\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"663\" height=\"1013\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" class=\"attachment-large size-large wp-image-63513\" alt=\"gelatin effect - Time to plateau\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?w=663&amp;ssl=1 663w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?resize=196%2C300&amp;ssl=1 196w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?resize=49%2C75&amp;ssl=1 49w\" sizes=\"(max-width:767px) 663px, 663px\" data-attachment-id=\"63513\" data-permalink=\"https:\/\/rheolution.com\/pt\/gelatin-effect-time-to-plateau\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" data-orig-size=\"663,1013\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-65db86c elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"65db86c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 4.<\/b><span style=\"font-weight: 400;\">  Tempo at\u00e9 a gelifica\u00e7\u00e3o, definido pelo ponto de transi\u00e7\u00e3o G\u2032\u2013G\u2033 (\u00e0 esquerda), e tempo para atingir o patamar viscoel\u00e1stico (\u00e0 direita; tempo para que G\u2032 atinja 90% de seu valor final estabilizado) para 5, 10 e 15% em peso de gelatina reticulada com transglutaminase (30 U g\u207b\u00b9 a 45 \u00b0C). As barras representam a m\u00e9dia \u00b1 DP, com as r\u00e9plicas individuais sobrepostas. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como *p &lt; 0,05, **p&lt; 0,01, ***p &lt; 0,001; ns, n\u00e3o significativo.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-794c36a elementor-widget elementor-widget-heading\" data-id=\"794c36a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Efeito da concentra\u00e7\u00e3o de transglutaminase na gelifica\u00e7\u00e3o e nas propriedades mec\u00e2nicas<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aed2e2d elementor-widget elementor-widget-text-editor\" data-id=\"aed2e2d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">A Figura 5 ilustra a evolu\u00e7\u00e3o em fun\u00e7\u00e3o do tempo do m\u00f3dulo de armazenamento de cisalhamento (G\u2032) durante a reticula\u00e7\u00e3o da gelatina com transglutaminase microbiana em duas concentra\u00e7\u00f5es enzim\u00e1ticas diferentes. A linha cont\u00ednua corresponde \u00e0 m\u00e9dia de medi\u00e7\u00f5es realizadas em triplicata, enquanto as linhas tracejadas indicam o desvio padr\u00e3o. Assim como anteriormente, o G\u2032 permaneceu baixo inicialmente e, em seguida, aumentou acentuadamente ap\u00f3s a transi\u00e7\u00e3o l\u00edquido-gel, atingindo, por fim, um patamar est\u00e1vel.  <\/span><\/p><p><span style=\"font-weight: 400;\">A Figura 6 mostra os valores m\u00e9dios de G\u2032 e G\u2033 durante a \u00faltima hora do teste. O valor final de G\u2032 aumentou com o aumento da concentra\u00e7\u00e3o enzim\u00e1tica (**p &lt; 0,01), uma vez que n\u00edveis mais elevados de enzima permitem uma maior forma\u00e7\u00e3o de liga\u00e7\u00f5es cruzadas no mesmo per\u00edodo de avalia\u00e7\u00e3o. N\u00e3o foram observadas diferen\u00e7as estat\u00edsticas para G\u2019\u2019 e tan \u03b4.  <\/span><\/p><p><span style=\"font-weight: 400;\">A Figura 7 mostra o tempo necess\u00e1rio para atingir o ponto de transi\u00e7\u00e3o l\u00edquido-gel (\u00e0 esquerda) e o tempo total necess\u00e1rio para atingir o plat\u00f4 (\u00e0 direita). Como esperado, a condi\u00e7\u00e3o com maior concentra\u00e7\u00e3o enzim\u00e1tica exigiu menos tempo para atingir o ponto de transi\u00e7\u00e3o l\u00edquido-gel devido ao aumento da atividade de reticula\u00e7\u00e3o. Nesse caso, o tempo necess\u00e1rio para atingir o plat\u00f4 tamb\u00e9m foi significativamente menor em concentra\u00e7\u00f5es enzim\u00e1ticas mais elevadas, corroborando a constata\u00e7\u00e3o anterior de que esse par\u00e2metro \u00e9 mais fortemente influenciado pela concentra\u00e7\u00e3o enzim\u00e1tica do que pela concentra\u00e7\u00e3o de gelatina.  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a99d963 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a99d963\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"639\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1024%2C639&amp;ssl=1\" class=\"attachment-large size-large wp-image-63602\" alt=\"15%, 10% compare t300 - G&apos; kinetics\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?w=1563&amp;ssl=1 1563w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=300%2C187&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=1024%2C639&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=768%2C480&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=1536%2C959&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=120%2C75&amp;ssl=1 120w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=960%2C600&amp;ssl=1 960w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=700%2C437&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63602\" data-permalink=\"https:\/\/rheolution.com\/pt\/15-10-compare-t300-g-kinetics\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1563%2C976&amp;ssl=1\" data-orig-size=\"1563,976\" data-comments-opened=\"1\" data-image-title=\"15%, 10% compare t300 &amp;#8211; G&amp;#8217; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1024%2C639&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f1ddc8d elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f1ddc8d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 5.<\/b><span style=\"font-weight: 400;\">  Evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento ao cisalhamento (G\u2032) durante a gelifica\u00e7\u00e3o de hidrog\u00e9is de gelatina a 10% em peso, reticulados a 45 \u00b0C com 30 U g\u207b\u00b9 e 15 U g\u207b\u00b9 de transglutaminase.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-8b274f3 e-grid e-con-full e-con e-child\" data-id=\"8b274f3\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fc14b22 elementor-widget elementor-widget-image\" data-id=\"fc14b22\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"750\" height=\"917\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" class=\"attachment-large size-large wp-image-63562\" alt=\"tg effect - G&apos; Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?w=750&amp;ssl=1 750w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=245%2C300&amp;ssl=1 245w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=61%2C75&amp;ssl=1 61w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=700%2C856&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 750px\" data-attachment-id=\"63562\" data-permalink=\"https:\/\/rheolution.com\/pt\/tg-effect-g-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" data-orig-size=\"750,917\" data-comments-opened=\"1\" data-image-title=\"tg effect &amp;#8211; G&amp;#8217; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1c23c75 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"1c23c75\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"667\" height=\"905\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" class=\"attachment-large size-large wp-image-63570\" alt=\"tg effect- G&apos;&apos; Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?w=667&amp;ssl=1 667w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?resize=221%2C300&amp;ssl=1 221w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?resize=55%2C75&amp;ssl=1 55w\" sizes=\"(max-width:767px) 667px, 667px\" data-attachment-id=\"63570\" data-permalink=\"https:\/\/rheolution.com\/pt\/tg-effect-g-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" data-orig-size=\"667,905\" data-comments-opened=\"1\" data-image-title=\"tg effect- G&amp;#8221; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6ec4683 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"6ec4683\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"688\" height=\"888\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" class=\"attachment-large size-large wp-image-63586\" alt=\"tg effect- TanD Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?w=688&amp;ssl=1 688w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?resize=232%2C300&amp;ssl=1 232w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?resize=58%2C75&amp;ssl=1 58w\" sizes=\"(max-width:767px) 688px, 688px\" data-attachment-id=\"63586\" data-permalink=\"https:\/\/rheolution.com\/pt\/tg-effect-tand-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" data-orig-size=\"688,888\" data-comments-opened=\"1\" data-image-title=\"tg effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f788b5a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f788b5a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 6.<\/b><span style=\"font-weight: 400;\">  Propriedades viscoel\u00e1sticas finais de hidrog\u00e9is de gelatina reticulados com transglutaminase a 45 \u00b0C. O m\u00f3dulo de armazenamento ao cisalhamento (G\u2032, \u00e0 esquerda), o m\u00f3dulo de perda ao cisalhamento (G\u2033, no meio), e o fator de amortecimento (tan \u03b4, \u00e0 direita) foram calculados a partir da regi\u00e3o de plat\u00f4 (5\u20136 h) das curvas de gelifica\u00e7\u00e3o para hidrog\u00e9is de gelatina a 10% em peso reticulados com 30 U g\u207b\u00b9 e 15 U g\u207b\u00b9 de transglutaminase. As barras mostram a m\u00e9dia \u00b1 DP, com pontos de dados individuais sobrepostos. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como ***p &lt; 0,001; ns, n\u00e3o significativo.   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-989a981 e-grid e-con-full e-con e-child\" data-id=\"989a981\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-108df9d elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"108df9d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"705\" height=\"926\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" class=\"attachment-large size-large wp-image-63578\" alt=\"tg effect- sol-gel transition\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?w=705&amp;ssl=1 705w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=228%2C300&amp;ssl=1 228w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=57%2C75&amp;ssl=1 57w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=700%2C919&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 705px\" data-attachment-id=\"63578\" data-permalink=\"https:\/\/rheolution.com\/pt\/tg-effect-sol-gel-transition\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" data-orig-size=\"705,926\" data-comments-opened=\"1\" data-image-title=\"tg effect- sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53efe65 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"53efe65\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"663\" height=\"942\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" class=\"attachment-large size-large wp-image-63594\" alt=\"tg effect- Time to plateau\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?w=663&amp;ssl=1 663w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?resize=211%2C300&amp;ssl=1 211w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?resize=53%2C75&amp;ssl=1 53w\" sizes=\"(max-width:767px) 663px, 663px\" data-attachment-id=\"63594\" data-permalink=\"https:\/\/rheolution.com\/pt\/tg-effect-time-to-plateau\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" data-orig-size=\"663,942\" data-comments-opened=\"1\" data-image-title=\"tg effect- Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-26f2837 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"26f2837\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 7.<\/b><span style=\"font-weight: 400;\">  Tempo at\u00e9 a gelifica\u00e7\u00e3o, definido pelo ponto de transi\u00e7\u00e3o G\u2032\u2013G\u2033 (\u00e0 esquerda), e tempo para atingir o patamar viscoel\u00e1stico (\u00e0 direita; tempo para que G\u2032 atinja 90% de seu valor final estabilizado) para gelatina a 10% em peso reticulada com transglutaminase (a 45 \u00b0C) a 30 U g\u207b\u00b9 e 15 U g\u207b\u00b9. As barras representam a m\u00e9dia \u00b1 desvio padr\u00e3o, com as r\u00e9plicas individuais sobrepostas. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como *p &lt; 0,05.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7af856 elementor-widget elementor-widget-heading\" data-id=\"b7af856\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Efeito da temperatura na gelifica\u00e7\u00e3o e nas propriedades mec\u00e2nicas<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afd54d4 elementor-widget elementor-widget-text-editor\" data-id=\"afd54d4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">De forma semelhante aos resultados anteriores, as Figuras 8, 9 e 10 mostram a evolu\u00e7\u00e3o temporal do G\u2032 durante a reticula\u00e7\u00e3o da gelatina, os valores finais de G\u2032 e G\u2033, e o tempo necess\u00e1rio para atingir o ponto de transi\u00e7\u00e3o e o plat\u00f4, desta vez para duas condi\u00e7\u00f5es que diferem nas temperaturas de teste (37 \u00b0C e 45 \u00b0C). Embora tenha sido observada uma ligeira diferen\u00e7a na cin\u00e9tica de reticula\u00e7\u00e3o, n\u00e3o foram encontradas diferen\u00e7as estatisticamente significativas em nenhum dos par\u00e2metros avaliados.   <\/span><\/p><p><span style=\"font-weight: 400;\">\u00c9 importante observar que, a 37 \u00b0C, n\u00e3o foi poss\u00edvel determinar o tempo necess\u00e1rio para atingir o ponto de gelifica\u00e7\u00e3o em duas das tr\u00eas amostras, uma vez que a gelifica\u00e7\u00e3o j\u00e1 havia ocorrido antes do in\u00edcio do teste. A essa temperatura, tamb\u00e9m pode ocorrer gelifica\u00e7\u00e3o t\u00e9rmica, o que poderia acelerar o processo de gelifica\u00e7\u00e3o e levar a esse resultado. Consequentemente, pode existir uma diferen\u00e7a estatisticamente significativa nesse par\u00e2metro (tempo at\u00e9 a gelifica\u00e7\u00e3o) entre essas condi\u00e7\u00f5es (37 \u00b0C versus 45 \u00b0C).    <\/span><\/p><p><span style=\"font-weight: 400;\">Para aplica\u00e7\u00f5es biom\u00e9dicas, os resultados indicam que essas formula\u00e7\u00f5es podem ser processadas em temperaturas fisiol\u00f3gicas, mantendo atividade enzim\u00e1tica e propriedades viscoel\u00e1sticas finais semelhantes, o que \u00e9 vantajoso para seu uso pr\u00e1tico.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-56855da elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"56855da\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"655\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1024%2C655&amp;ssl=1\" class=\"attachment-large size-large wp-image-63638\" alt=\"15%, 5% compare temp - G&apos; kinetics\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?w=1500&amp;ssl=1 1500w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=300%2C192&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=1024%2C655&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=768%2C491&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=117%2C75&amp;ssl=1 117w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=700%2C448&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63638\" data-permalink=\"https:\/\/rheolution.com\/pt\/15-5-compare-temp-g-kinetics\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1500%2C959&amp;ssl=1\" data-orig-size=\"1500,959\" data-comments-opened=\"1\" data-image-title=\"15%, 5% compare temp &amp;#8211; G&amp;#8217; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1024%2C655&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afc49b7 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"afc49b7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 8.<\/b><span style=\"font-weight: 400;\">  Evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento ao cisalhamento (G\u2032) durante a gelifica\u00e7\u00e3o de hidrog\u00e9is de gelatina a 15% em peso, reticulados com transglutaminase (30 U g\u207b\u00b9) a 37 \u00b0C e 45 \u00b0C.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1eb37cc e-grid e-con-full e-con e-child\" data-id=\"1eb37cc\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-47cba32 elementor-widget elementor-widget-image\" data-id=\"47cba32\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"867\" height=\"750\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" class=\"attachment-large size-large wp-image-63654\" alt=\"temp effect - G&apos; Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?w=867&amp;ssl=1 867w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=300%2C260&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=768%2C664&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=87%2C75&amp;ssl=1 87w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=700%2C606&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:867px) 100vw, 867px\" data-attachment-id=\"63654\" data-permalink=\"https:\/\/rheolution.com\/pt\/temp-effect-g-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" data-orig-size=\"867,750\" data-comments-opened=\"1\" data-image-title=\"temp effect &amp;#8211; G&amp;#8217; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bd60dae elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"bd60dae\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"784\" height=\"788\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" class=\"attachment-large size-large wp-image-63646\" alt=\"g&apos;&apos; temp effect bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?w=784&amp;ssl=1 784w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=298%2C300&amp;ssl=1 298w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=768%2C772&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=75%2C75&amp;ssl=1 75w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=700%2C704&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:784px) 100vw, 784px\" data-attachment-id=\"63646\" data-permalink=\"https:\/\/rheolution.com\/pt\/g-temp-effect-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" data-orig-size=\"784,788\" data-comments-opened=\"1\" data-image-title=\"g&amp;#8221; temp effect bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c4e05d8 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"c4e05d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"805\" height=\"750\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" class=\"attachment-large size-large wp-image-63630\" alt=\"temp effect- TanD Bar graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?w=805&amp;ssl=1 805w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=300%2C280&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=768%2C716&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=81%2C75&amp;ssl=1 81w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=700%2C652&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:805px) 100vw, 805px\" data-attachment-id=\"63630\" data-permalink=\"https:\/\/rheolution.com\/pt\/temp-effect-tand-bar-graph\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" data-orig-size=\"805,750\" data-comments-opened=\"1\" data-image-title=\"temp effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e09e2fc elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"e09e2fc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 9.<\/b><span style=\"font-weight: 400;\">  Propriedades viscoel\u00e1sticas finais de hidrog\u00e9is de gelatina reticulados com transglutaminase a 37 \u00b0C e 45 \u00b0C. O m\u00f3dulo de armazenamento ao cisalhamento (G\u2032, \u00e0 esquerda), o m\u00f3dulo de perda de cisalhamento (G\u2033, no meio) e o fator de amortecimento (tan \u03b4, \u00e0 direita) foram calculados a partir da regi\u00e3o de plat\u00f4 (5\u20136 h) das curvas de gelifica\u00e7\u00e3o para hidrog\u00e9is de gelatina a 15% em peso reticulados com 30 U g\u207b\u00b9 de transglutaminase. As barras mostram a m\u00e9dia \u00b1 DP, com pontos de dados individuais sobrepostos. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como ns, n\u00e3o significativo.   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c25765a e-grid e-con-full e-con e-child\" data-id=\"c25765a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2b74930 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"2b74930\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"871\" height=\"771\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" class=\"attachment-large size-large wp-image-63622\" alt=\"temp effect- sol-gel transition\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?w=871&amp;ssl=1 871w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=300%2C266&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=768%2C680&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=85%2C75&amp;ssl=1 85w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=700%2C620&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:871px) 100vw, 871px\" data-attachment-id=\"63622\" data-permalink=\"https:\/\/rheolution.com\/pt\/temp-effect-sol-gel-transition\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" data-orig-size=\"871,771\" data-comments-opened=\"1\" data-image-title=\"temp effect- sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-562ab31 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"562ab31\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"780\" height=\"780\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" class=\"attachment-large size-large wp-image-63614\" alt=\"temp effect min- Time to plateau\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?w=780&amp;ssl=1 780w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=300%2C300&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=768%2C768&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=75%2C75&amp;ssl=1 75w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=700%2C700&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:780px) 100vw, 780px\" data-attachment-id=\"63614\" data-permalink=\"https:\/\/rheolution.com\/pt\/temp-effect-min-time-to-plateau\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" data-orig-size=\"780,780\" data-comments-opened=\"1\" data-image-title=\"temp effect min- Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bc7f5a7 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"bc7f5a7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figura 10.<\/b><span style=\"font-weight: 400;\">  Tempo de gelifica\u00e7\u00e3o, definido pelo ponto de transi\u00e7\u00e3o G\u2032\u2013G\u2033 (\u00e0 esquerda), e tempo para atingir o patamar viscoel\u00e1stico (\u00e0 direita; tempo para que G\u2032 atinja 90% de seu valor final estabilizado) para gelatina a 15% em peso reticulada com transglutaminase (30 U g\u207b\u00b9) a 37 \u00b0C e 45 \u00b0C. As barras representam a m\u00e9dia \u00b1 DP com r\u00e9plicas individuais sobrepostas. A signific\u00e2ncia estat\u00edstica \u00e9 indicada como ns, n\u00e3o significativo.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8ad5968 elementor-widget elementor-widget-heading\" data-id=\"8ad5968\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Conclus\u00f5es e perspectivas<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7d7a98 elementor-widget elementor-widget-text-editor\" data-id=\"b7d7a98\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Este estudo demonstra que as propriedades mec\u00e2nicas do hidrogel de gelatina e a cin\u00e9tica de gelifica\u00e7\u00e3o podem ser ajustadas com precis\u00e3o por meio de par\u00e2metros de formula\u00e7\u00e3o e monitoradas quantitativamente em tempo real utilizando o ElastoSens\u2122 Bio. \u00c9 importante ressaltar que a reticula\u00e7\u00e3o enzim\u00e1tica oferece uma estrat\u00e9gia robusta para estabilizar hidrog\u00e9is \u00e0 base de gelatina \u00e0 temperatura fisiol\u00f3gica, superando as limita\u00e7\u00f5es dos g\u00e9is f\u00edsicos termicamente revers\u00edveis e possibilitando seu uso em condi\u00e7\u00f5es biologicamente relevantes. A capacidade de realizar a reticula\u00e7\u00e3o enzim\u00e1tica \u00e0 temperatura fisiol\u00f3gica, ao mesmo tempo em que se obt\u00eam propriedades mec\u00e2nicas reproduz\u00edveis e control\u00e1veis, destaca a relev\u00e2ncia dessa plataforma para o desenvolvimento de biomateriais, a triagem de formula\u00e7\u00f5es e o controle de qualidade.  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9383078 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"9383078\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-c97f30c swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"elastosens2\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8cce0c6 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"8cce0c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Descubra como nossa tecnologia mede, de forma n\u00e3o destrutiva, as propriedades viscoel\u00e1sticas de biomateriais e tecidos moles utilizando microvolumes de amostras<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c4ce1d0 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"c4ce1d0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/pt\/contato\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Entre em contato conosco para mais informa\u00e7\u00f5es<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-efbb293 elementor-widget elementor-widget-heading\" data-id=\"efbb293\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Posts Relacionados<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f623a9 premium-blog-cta-full-yes 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https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7000px) 100vw, 7000px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-fibrina\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hidrog\u00e9is de fibrina<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-fibrina\/\" target=\"_blank\">\n\t\t\t\tHidrog\u00e9is de fibrina\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Os hidrog\u00e9is de fibrina s\u00e3o biomateriais naturais \u00e0 base de prote\u00ednas, formados a partir da fibrina, o pol\u00edmero insol\u00favel gerado durante a coagula\u00e7\u00e3o sangu\u00ednea. A fibrina tem origem no fibrinog\u00eanio, uma glicoprote\u00edna plasm\u00e1tica composta por tr\u00eas cadeias polipept\u00eddicas emparelhadas que se organizam em uma rede fibrosa ap\u00f3s ativa\u00e7\u00e3o enzim\u00e1tica. Em condi\u00e7\u00f5es fisiol\u00f3gicas, a forma\u00e7\u00e3o da fibrina \u00e9 desencadeada pela clivagem do fibrinog\u00eanio mediada pela trombina, expondo s\u00edtios de polimeriza\u00e7\u00e3o que impulsionam a auto-montagem espont\u00e2nea em uma matriz hidratada e porosa.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-fibrina\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7000\" height=\"2322\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=7000%2C2322&amp;ssl=1\" class=\"attachment-full size-full wp-image-65612\" alt=\"Alginate Hydrogel\" data-attachment-id=\"65612\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-alginato\/attachment\/alginate-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=7000%2C2322&amp;ssl=1\" data-orig-size=\"7000,2322\" data-comments-opened=\"1\" data-image-title=\"ALGINATE\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=7000&amp;ssl=1 7000w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=1536%2C510&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7000px) 100vw, 7000px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-alginato\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hidrog\u00e9is de alginato<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-alginato\/\" target=\"_blank\">\n\t\t\t\tHidrog\u00e9is de alginato\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Os hidrog\u00e9is de alginato s\u00e3o redes polim\u00e9ricas tridimensionais ricas em \u00e1gua, derivadas do alginato, um polissacar\u00eddeo ani\u00f4nico de origem natural. O alginato \u00e9 extra\u00eddo principalmente das paredes celulares das algas marrons pertencentes \u00e0 classe Phaeophyceae, embora tamb\u00e9m possa ser biossintetizado por certas esp\u00e9cies bacterianas, como Azotobacter e Pseudomonas. Estruturalmente, o alginato \u00e9 um copol\u00edmero linear composto por \u00e1cido \u03b2-D-manur\u00f4nico (unidades M) e \u00e1cido \u03b1-L-gulur\u00f4nico (unidades G), ligados por liga\u00e7\u00f5es glicos\u00eddicas 1\u21924.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/hidrogeis-de-alginato\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7875\" height=\"2612\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=7875%2C2612&amp;ssl=1\" class=\"attachment-full size-full wp-image-65567\" alt=\"Gelatin Methacrylate - GelMa\" data-attachment-id=\"65567\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/gelma-hidrogeis\/attachment\/gelatin-methacrylate-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=7875%2C2612&amp;ssl=1\" data-orig-size=\"7875,2612\" data-comments-opened=\"1\" data-image-title=\"Gelatin Methacrylate\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=7875&amp;ssl=1 7875w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=1536%2C509&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7875px) 100vw, 7875px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/gelma-hidrogeis\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hidrog\u00e9is GelMA<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/gelma-hidrogeis\/\" target=\"_blank\">\n\t\t\t\tHidrog\u00e9is GelMA\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">A gelatina metacril\u00f3il (GelMA) \u00e9 um hidrogel fotocrosslink\u00e1vel derivado da gelatina, que, por sua vez, \u00e9 obtida por hidr\u00f3lise parcial do col\u00e1geno, a principal prote\u00edna estrutural da matriz extracelular (MEC). A GelMA \u00e9 sintetizada por meio da rea\u00e7\u00e3o da gelatina com o anidrido metacr\u00edlico, introduzindo grupos funcionais metacril\u00f3is nos res\u00edduos de amino\u00e1cidos da gelatina, ao mesmo tempo em que preserva a maior parte de seus motivos bioativos nativos.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/gelma-hidrogeis\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7875\" height=\"2612\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=7875%2C2612&amp;ssl=1\" class=\"attachment-full size-full wp-image-65405\" alt=\"Polydimethylsiloxane (PDMS)\" data-attachment-id=\"65405\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/pdms-polimeros\/attachment\/polydimethylsiloxane-pdms-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=7875%2C2612&amp;ssl=1\" data-orig-size=\"7875,2612\" data-comments-opened=\"1\" data-image-title=\"Polydimethylsiloxane (PDMS)\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=7875&amp;ssl=1 7875w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=1536%2C509&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7875px) 100vw, 7875px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/pdms-polimeros\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Pol\u00edmeros PDMS<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/pdms-polimeros\/\" target=\"_blank\">\n\t\t\t\tPol\u00edmeros PDMS\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">O polidimetilsiloxano (PDMS) \u00e9 um pol\u00edmero polissiloxano sint\u00e9tico composto por uma estrutura principal flex\u00edvel de siloxano inorg\u00e2nico (\u2013Si\u2013O\u2013Si\u2013) com grupos laterais metil. Essa arquitetura inorg\u00e2nico-org\u00e2nica \u00fanica confere ao PDMS excepcional mobilidade de cadeia, temperatura de transi\u00e7\u00e3o v\u00edtrea muito baixa e comportamento elastom\u00e9rico est\u00e1vel em uma ampla faixa de temperatura. O PDMS \u00e9 produzido exclusivamente por s\u00edntese industrial, envolvendo tipicamente a hidr\u00f3lise e a condensa\u00e7\u00e3o de clorosilanos, seguidas pela polimeriza\u00e7\u00e3o por abertura de anel de siloxanos c\u00edclicos.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/pdms-polimeros\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leia Mais \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t<div class=\"premium-blog-footer\">\n\t\t\t\t\t\t\t<nav class=\"premium-blog-pagination-container\" role=\"navigation\" aria-label=\"Pagina\u00e7\u00e3o\">\n\t\t\t\t<span aria-current=\"page\" class=\"page-numbers current\">1<\/span>\n<a class=\"page-numbers\" href=\"https:\/\/rheolution.com\/pt\/wp-json\/wp\/v2\/posts\/70913\/page\/2\/\">2<\/a>\n<a class=\"page-numbers\" href=\"https:\/\/rheolution.com\/pt\/wp-json\/wp\/v2\/posts\/70913\/page\/3\/\">3<\/a>\n<a class=\"next page-numbers\" href=\"https:\/\/rheolution.com\/pt\/wp-json\/wp\/v2\/posts\/70913\/page\/2\/\">Pr\u00f3ximo \u00bb<\/a>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>A gelatina \u00e9 um biopol\u00edmero amplamente utilizado em biomateriais, pois pode ser processada em \u00e1gua, \u00e9 biocompat\u00edvel e \u00e9 capaz de formar redes macias e hidratadas. No entanto, os g\u00e9is f\u00edsicos de gelatina podem enfraquecer ou derreter pr\u00f3ximas \u00e0s temperaturas fisiol\u00f3gicas; por isso, a reticula\u00e7\u00e3o covalente \u00e9 comumente utilizada para melhorar a estabilidade t\u00e9rmica e a integridade mec\u00e2nica. A X-Pure Gelatin\u00ae \u00e9 uma gelatina de alta qualidade, de grau farmac\u00eautico, caracterizada por rigorosos padr\u00f5es de pureza e desempenho consistente.  <\/p>\n","protected":false},"author":195401359,"featured_media":70914,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[286,299],"tags":[89,91,124,88],"class_list":["post-70913","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-notas-de-aplicacao","category-application-formulation-of-hydrogels","tag-elastosens-bio","tag-hydrogels","tag-life-sciences","tag-mechanical-testing"],"yoast_head":"<!-- This site is optimized with the 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