{"id":70810,"date":"2026-09-29T11:28:51","date_gmt":"2026-09-29T15:28:51","guid":{"rendered":"https:\/\/rheolution.com\/?p=70810"},"modified":"2026-09-29T11:28:51","modified_gmt":"2026-09-29T15:28:51","slug":"viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel","status":"publish","type":"post","link":"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/","title":{"rendered":"Ajuste da rigidez dos hidrog\u00e9is de ECM"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70810\" class=\"elementor elementor-70810 elementor-19680\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9926633 e-flex e-con-boxed e-con e-parent\" data-id=\"9926633\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2aa8b2e elementor-widget elementor-widget-heading\" data-id=\"2aa8b2e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota de Aplica\u00e7\u00e3o | ElastoSens\u2122 Bio<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b490e4b elementor-widget elementor-widget-heading\" data-id=\"b490e4b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Ajuste da rigidez dos hidrog\u00e9is de ECM com o ElastoSens\u2122 Bio<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ed01f46 elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"ed01f46\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Em colabora\u00e7\u00e3o com<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-989a838 elementor-widget elementor-widget-image\" data-id=\"989a838\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.gelomics.com\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"80\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=300%2C80&amp;ssl=1\" class=\"attachment-medium size-medium wp-image-70811\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=9178&amp;ssl=1 9178w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=300%2C80&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1024%2C272&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=768%2C204&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1536%2C407&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=2048%2C543&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1200%2C318&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=260%2C69&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=50%2C13&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=150%2C40&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 300px, 300px\" data-attachment-id=\"70811\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/gelomics_logo_horizontal_with_claim_full-colour_181002-01-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=9178%2C2434&amp;ssl=1\" data-orig-size=\"9178,2434\" data-comments-opened=\"1\" data-image-title=\"Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=1024%2C272&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9026f26 elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"9026f26\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Nota<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3db1a2 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"d3db1a2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Este estudo foi uma colabora\u00e7\u00e3o conjunta entre <a href=\"https:\/\/rheolution.com\/life-sciences\/\">a Rheolution<\/a> (QC, Canad\u00e1) e <a href=\"https:\/\/www.gelomics.com\/hydrogels\">a Gelomics<\/a> (QLD, Austr\u00e1lia).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3ef1a56 elementor-widget elementor-widget-text-editor\" data-id=\"3ef1a56\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"raw_components--panel--3IcXg inspect_panels--inspectionPanel--3Wboz\" tabindex=\"-1\"><div class=\"inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--propertyRowContent--7Y-mu inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--contentProperty--2Z1QI text--fontPos11--RSei3 text--_fontBase--YWDo0 ellipsis--ellipsisAfter8Lines--2rn32 ellipsis--_ellipsisAfterNLines--26JkZ\"><p>por <a href=\"https:\/\/www.linkedin.com\/in\/gloriapinilla\/\" target=\"_blank\" rel=\"noopener\">Gloria Pinilla, M.Sc.<\/a>, Especialista S\u00eanior em Aplica\u00e7\u00f5es da Rheolution Inc.<\/p><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-29c9a4e elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"29c9a4e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resumo<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23a78fe elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"23a78fe\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>A rigidez inicial dos sistemas de cultura 3D pode ser ajustada para estudar processos fisiol\u00f3gicos e patol\u00f3gicos in vitro.<\/li><li>Os hidrog\u00e9is fotocrosslink\u00e1veis s\u00e3o excelentes candidatos para sistemas de cultura 3D devido \u00e0 sua semelhan\u00e7a com a matriz extracelular natural e \u00e0 facilidade de controlar sua rigidez final.<\/li><li>Os produtos de matriz extracelular LunaGel\u2122 da Gelomics foram fotoreticulados com sucesso com luz vis\u00edvel, e sua cin\u00e9tica de reticula\u00e7\u00e3o foi medida em tempo real no <a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a>.<\/li><li>As rea\u00e7\u00f5es de fotocrosslinking ocorreram rapidamente tanto nos kits LunaGel\u2122 de baixa quanto nos de alta rigidez.<\/li><li>Os kits LunaGel\u2122 de alta rigidez apresentaram maior rigidez do que os kits de baixa rigidez.<\/li><li>A rigidez das matrizes de LunaGel\u2122 aumentou com o tempo de fotoreticula\u00e7\u00e3o.<\/li><li>A invasividade das c\u00e9lulas do c\u00e2ncer de mama aumentou significativamente com o aumento da rigidez da MEC.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a11b72f e-flex e-con-boxed e-con e-parent\" data-id=\"a11b72f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ff67a5f elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"ff67a5f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">O papel da rigidez do hidrogel no comportamento celular\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ddf558e elementor-widget elementor-widget-heading\" data-id=\"ddf558e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Introdu\u00e7\u00e3o<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dcef2a1 elementor-widget elementor-widget-text-editor\" data-id=\"dcef2a1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Todas as c\u00e9lulas do corpo humano est\u00e3o expostas a for\u00e7as mec\u00e2nicas que regulam a fun\u00e7\u00e3o celular e o desenvolvimento dos tecidos, e cada tipo de c\u00e9lula est\u00e1 especificamente adaptado \u00e0s propriedades mec\u00e2nicas do tecido em que se encontra. As propriedades da matriz dos tecidos humanos tamb\u00e9m podem se alterar com a doen\u00e7a e, por sua vez, facilitar sua progress\u00e3o. Por exemplo, o crescimento, a sobreviv\u00eancia, a diferencia\u00e7\u00e3o e a morfog\u00eanese das c\u00e9lulas epiteliais mam\u00e1rias normais s\u00e3o amplamente favorecidos pela intera\u00e7\u00e3o com uma matriz macia, semelhante \u00e0 rigidez do tecido mam\u00e1rio normal. Ap\u00f3s a transforma\u00e7\u00e3o durante o c\u00e2ncer de mama, no entanto, o tecido torna-se progressivamente mais r\u00edgido [1] e as c\u00e9lulas tumorais tornam-se significativamente mais contr\u00e1teis e hiper-responsivas aos est\u00edmulos mec\u00e2nicos da matriz, levando, em \u00faltima inst\u00e2ncia, \u00e0 transi\u00e7\u00e3o epitelial-mesenquimal (EMT) e \u00e0 met\u00e1stase [2,3]. A investiga\u00e7\u00e3o mecanicista da complexa intera\u00e7\u00e3o entre as propriedades do substrato e o comportamento celular requer metodologias avan\u00e7adas de cultura celular que superem as limita\u00e7\u00f5es associadas \u00e0s abordagens tradicionais de cultura [4].    <\/p><p>As matrizes extracelulares (ECMs) LunaGel\u2122 da Gelomics s\u00e3o hidrog\u00e9is fotocrosslink\u00e1veis que proporcionam um sistema controlado de cultura celular 3D para o estudo de processos patol\u00f3gicos e fisiol\u00f3gicos <i>in vitro<\/i>. Baseadas em componentes da MCE, como col\u00e1geno dos tipos I, III, IV e V, bem como glicoprote\u00ednas e proteoglicanos do tecido conjuntivo, essas MCE imitam fielmente a matriz extracelular (MCE) natural que envolve as c\u00e9lulas no corpo humano e facilitam a ades\u00e3o, prolifera\u00e7\u00e3o, diferencia\u00e7\u00e3o e migra\u00e7\u00e3o celular, al\u00e9m da degrada\u00e7\u00e3o proteol\u00edtica da MCE. Al\u00e9m disso, o processo de reticula\u00e7\u00e3o fotoqu\u00edmica permite que os cientistas tenham maior controle sobre a rigidez final do gel. Simplesmente variando a dura\u00e7\u00e3o da reticula\u00e7\u00e3o, a rigidez do gel pode ser ajustada com precis\u00e3o para imitar tecidos moles e duros, como o c\u00e9rebro, o f\u00edgado, a mama e a cartilagem, entre outros, tanto em condi\u00e7\u00f5es saud\u00e1veis quanto patol\u00f3gicas. Por essas raz\u00f5es, eles s\u00e3o substratos ideais para a cultura e diferencia\u00e7\u00e3o de uma ampla variedade de tipos de c\u00e9lulas e facilitam a an\u00e1lise das altera\u00e7\u00f5es no comportamento celular induzidas pela rigidez da matriz.      <\/p><p><a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\" target=\"_blank\" rel=\"noopener\">O ElastoSens\u2122 Bio<\/a> mede, sem contato, as propriedades viscoel\u00e1sticas de materiais macios. Ao aplicar vibra\u00e7\u00f5es suaves \u00e0 amostra, o instrumento \u00e9 capaz de registrar a cin\u00e9tica de fotoreticula\u00e7\u00e3o no comprimento de onda desejado. Nesta nota de aplica\u00e7\u00e3o, os ECMs LunaGel\u2122, baseados em pele su\u00edna quimicamente modificada e gelatina de osso bovino, foram submetidos \u00e0 fotoreticula\u00e7\u00e3o, enquanto sua cin\u00e9tica de reticula\u00e7\u00e3o foi avaliada em tempo real no <a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a>. Para investigar a adequa\u00e7\u00e3o das MECs LunaGel\u2122 para simular altera\u00e7\u00f5es fisiopatol\u00f3gicas na rigidez tecidual e seus efeitos na progress\u00e3o do c\u00e2ncer, esfer\u00f3ides de c\u00e2ncer de mama foram cultivados em substratos de rigidez vari\u00e1vel e suas morfologias foram observadas.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cc16549 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"cc16549\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Fotoreticula\u00e7\u00e3o de hidrog\u00e9is LunaGel\u2122 pela Gelomics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cae21bc elementor-widget elementor-widget-heading\" data-id=\"cae21bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Materiais e M\u00e9todos<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dfb4356 elementor-widget elementor-widget-text-editor\" data-id=\"dfb4356\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Os ECMs LunaGel\u2122 \u00e0 base de gelatina de pele su\u00edna quimicamente modificada, tipo A (<a href=\"https:\/\/www.gelomics.com\/product-page\/porcine-skin-gelatin\">LunaGel\u2122 \u2013 Gelatina de Pele Su\u00edna \u2013 baixa e alta rigidez, Gelomics<\/a>) e gelatina de osso bovino, tipo B (<a href=\"https:\/\/www.gelomics.com\/product-page\/bovine-bone-gelatin\">LunaGel\u2122 \u2013 Gelatina de Osso Bovino \u2013 baixa e alta rigidez, Gelomics<\/a>), foram preparadas de acordo com as instru\u00e7\u00f5es do fabricante. Resumidamente, os frascos de fotoiniciador foram dissolvidos em PBS e misturados com as solu\u00e7\u00f5es de ECM fotocrosslink\u00e1veis a 37 \u00b0C. 2 mL dos produtos foram pipetados no porta-amostra do <a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a> e o teste de viscoelasticidade foi iniciado imediatamente a 37 \u00b0C, utilizando as seguintes sequ\u00eancias de medi\u00e7\u00e3o:  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3665319 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"3665319\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"304\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=1024%2C304&amp;ssl=1\" class=\"attachment-large size-large wp-image-70820\" alt=\"Table showing crossover of G\u2019 and G\u2019\u2019 indicating initial crosslinking in ElastoSens\u2122 Bio\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?w=2778&amp;ssl=1 2778w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=300%2C89&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1024%2C304&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=768%2C228&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1536%2C456&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=2048%2C607&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1200%2C356&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=260%2C77&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=50%2C15&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=150%2C44&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?w=2440&amp;ssl=1 2440w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"70820\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/screen-shot-2021-11-18-at-10-26-59-am-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=2778%2C824&amp;ssl=1\" data-orig-size=\"2778,824\" data-comments-opened=\"1\" data-image-title=\"crossover of G\u2019 and G\u2019\u2019\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=1024%2C304&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-10b03f2 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"10b03f2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>O m\u00f3dulo de elasticidade ao cisalhamento (G\u2019) e tan(\u03b4) foram obtidos diretamente do ElastoSens\u2122 Bio. O tempo inicial de reticula\u00e7\u00e3o foi determinado a partir do ponto de cruzamento entre G\u2019 e G\u2019\u2019 (tan(\u03b4) = 1). <\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0205664 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"0205664\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">A rigidez do hidrogel influencia o comportamento celular<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d2fcdb2 elementor-widget elementor-widget-heading\" data-id=\"d2fcdb2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Resultados e Discuss\u00e3o<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8b37e89 elementor-widget elementor-widget-text-editor\" data-id=\"8b37e89\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>As ECMs LunaGel\u2122 fotocrosslink\u00e1veis foram avaliadas inicialmente em termos de propriedades viscoel\u00e1sticas, que podem ser descritas pelo m\u00f3dulo de armazenamento ao cisalhamento (G\u2019 \u2014 por\u00e7\u00e3o el\u00e1stica do comportamento viscoel\u00e1stico) e pelo m\u00f3dulo de perda ao cisalhamento (G\u2019\u2019 \u2014 por\u00e7\u00e3o viscosa do comportamento viscoel\u00e1stico). A raz\u00e3o entre G\u2019\u2019 e G\u2019 (fator de amortecimento ou tan(\u03b4)) tamb\u00e9m \u00e9 normalmente determinada para indicar qual comportamento \u00e9 predominante.   <\/p><p><b>A Fig. 1<\/b> mostra a evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento de cisalhamento (G\u2019) em fun\u00e7\u00e3o do tempo de reticula\u00e7\u00e3o para MECs LunaGel\u2122 fotocrosslink\u00e1veis de baixa e alta rigidez, provenientes de su\u00ednos (\u00e0 esquerda) e bovinos (\u00e0 direita). As medi\u00e7\u00f5es foram realizadas sem exposi\u00e7\u00e3o \u00e0 luz durante os primeiros 2 minutos do teste. A luz foi ent\u00e3o programada para ser acesa pelo instrumento <a href=\"https:\/\/rheolution.com\/pt\/produtos\/elastosens-bio\/instrumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a> aos 2 minutos, e seu efeito foi claramente observado com o aumento de G\u2019 ap\u00f3s esse momento, at\u00e9 os 12 minutos (quando a luz foi desligada). As medi\u00e7\u00f5es foram continuadas por cerca de 15 minutos para identificar as propriedades viscoel\u00e1sticas finais do gel. As amostras apresentaram reticula\u00e7\u00e3o homog\u00eanea ap\u00f3s o teste (<b>Fig. 2<\/b>).    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e04bd87 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"e04bd87\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"861\" height=\"426\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" class=\"attachment-large size-large wp-image-70813\" alt=\"G\u2019 over time for LunaGel\u2122 ECMs: porcine (left) &amp; bovine (right)\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?w=861&amp;ssl=1 861w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=300%2C148&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=768%2C380&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=260%2C129&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=50%2C25&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=150%2C75&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:861px) 100vw, 861px\" data-attachment-id=\"70813\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/fig1_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" data-orig-size=\"861,426\" data-comments-opened=\"1\" data-image-title=\"G\u2019 over time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3a0f6a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"d3a0f6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 1:  <\/strong>M\u00f3dulo de armazenamento de cisalhamento (G\u2019, Pa) em fun\u00e7\u00e3o do tempo para os produtos LunaGel\u2122 ECM de origem su\u00edna (\u00e0 esquerda) e bovina (\u00e0 direita).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f58da9e elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"f58da9e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"825\" height=\"371\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" class=\"attachment-large size-large wp-image-70814\" alt=\"Crosslinked LunaGel\u2122 sample post-testing\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?w=825&amp;ssl=1 825w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=300%2C135&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=768%2C345&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=260%2C117&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=50%2C22&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=150%2C67&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:825px) 100vw, 825px\" data-attachment-id=\"70814\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/image-2-6\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" data-orig-size=\"825,371\" data-comments-opened=\"1\" data-image-title=\"Crosslinked LunaGel\u2122\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b3cbd35 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"b3cbd35\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 2:  <\/strong>Exemplo representativo de uma amostra de LunaGel\u2122 ap\u00f3s o teste.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ade62a3 elementor-widget elementor-widget-text-editor\" data-id=\"ade62a3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>A reticula\u00e7\u00e3o por luz teve in\u00edcio ap\u00f3s cerca de 0,5 min de exposi\u00e7\u00e3o \u00e0 luz para os hidrog\u00e9is de alta rigidez e cerca de 1,5 min para os de baixa rigidez (<b>Fig. 3<\/b>). O valor final de G\u2019 foi aproximadamente 8 vezes maior nas formula\u00e7\u00f5es de alta rigidez em compara\u00e7\u00e3o com as de baixa rigidez. As formula\u00e7\u00f5es de baixa rigidez formaram g\u00e9is macios com um G\u2019 de ~550 Pa, enquanto os hidrog\u00e9is de alta rigidez atingiram ~4.500 Pa, n\u00e3o tendo sido observada diferen\u00e7a estat\u00edstica entre as fontes su\u00edna e bovina. No entanto, foram observadas diferen\u00e7as significativas entre as formula\u00e7\u00f5es de baixa e alta rigidez para ambos os tipos de gelatina. De fato, os produtos LunaGel\u2122 de baixa e alta rigidez cont\u00eam concentra\u00e7\u00f5es diferentes dos componentes de gelatina modificada para permitir um controle preciso sobre uma ampla faixa de rigidez. O valor final de tan(\u03b4) foi sempre inferior a 0,1, demonstrando que o comportamento el\u00e1stico (relacionado ao m\u00f3dulo de armazenamento de cisalhamento, G\u2019\u2019) \u00e9 muito mais predominante do que o comportamento viscoso (relacionado ao m\u00f3dulo de perda de cisalhamento, G\u2019\u2019) nesses hidrog\u00e9is.     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f87206d elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"f87206d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1020\" height=\"401\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" class=\"attachment-large size-large wp-image-70815\" alt=\"Initial crosslinking time and final G\u2019\/tan(\u03b4) of LunaGel\u2122 gels\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?w=1020&amp;ssl=1 1020w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=300%2C118&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=768%2C302&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=260%2C102&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=50%2C20&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=150%2C59&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1020px) 100vw, 1020px\" data-attachment-id=\"70815\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/fig3_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" data-orig-size=\"1020,401\" data-comments-opened=\"1\" data-image-title=\"crosslinking time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a66f9b4 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"a66f9b4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 3:<\/strong>  Tempo inicial de reticula\u00e7\u00e3o (min), G\u2019 final (Pa) e tan(\u03b4) final dos g\u00e9is reticulados por fotorreticula\u00e7\u00e3o.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df11603 elementor-widget elementor-widget-text-editor\" data-id=\"df11603\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Tanto para formula\u00e7\u00f5es de baixa quanto de alta rigidez, a dura\u00e7\u00e3o da exposi\u00e7\u00e3o \u00e0 luz pode ser variada para atingir os valores-alvo de rigidez desejados, que sejam inferiores ou iguais ao valor final de G\u2019. <b>A Fig. 4<\/b> mostra a evolu\u00e7\u00e3o do m\u00f3dulo de armazenamento de cisalhamento (G\u2019, Pa) em fun\u00e7\u00e3o do tempo para o LunaGel\u2122 su\u00edno de alta rigidez, reticulado com 9,0 mW\/cm\u00b2 por 2, 4, 8 e 10 minutos, respectivamente. Da mesma forma, a luz foi programada para ser acesa ap\u00f3s 2 minutos de teste com a dura\u00e7\u00e3o mencionada. Como esperado, as curvas foram praticamente id\u00eanticas durante os primeiros 4 minutos. Ap\u00f3s esse ponto no tempo, o G\u2019 continuou a aumentar substancialmente com o tempo para as amostras submetidas a uma exposi\u00e7\u00e3o mais prolongada \u00e0 luz.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a255815 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a255815\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"431\" height=\"401\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" class=\"attachment-large size-large wp-image-70816\" alt=\"G\u2019 over time for high stiffness porcine LunaGel\u2122 with variable light exposure\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?w=431&amp;ssl=1 431w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=300%2C279&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=157%2C146&amp;ssl=1 157w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=50%2C47&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=81%2C75&amp;ssl=1 81w\" sizes=\"(max-width:767px) 431px, 431px\" data-attachment-id=\"70816\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/fig4_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" data-orig-size=\"431,401\" data-comments-opened=\"1\" data-image-title=\"G\u2019 over time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0635d67 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"0635d67\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 4:  <\/strong>M\u00f3dulo de armazenamento de cisalhamento (G\u2019, Pa) em fun\u00e7\u00e3o do tempo para o LunaGel\u2122 su\u00edno de alta rigidez, reticulado com luz de 405 nm (9,0 mW\/cm\u00b2) por 2, 4, 8 e 10 minutos.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9ca4a98 elementor-widget elementor-widget-text-editor\" data-id=\"9ca4a98\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Para examinar os efeitos da rigidez da MEC do LunaGel no comportamento das c\u00e9lulas de c\u00e2ncer de mama, esfer\u00f3ides de MDA-MB-231 foram semeados em MECs com m\u00f3dulo de armazenamento de cisalhamento (G\u2019) de ~ 110, 220 e 440 kPa, respectivamente. Ap\u00f3s 7 dias de cultura, observaram-se diferen\u00e7as substanciais nas morfologias celulares entre os diferentes n\u00edveis de rigidez da MEC (<b>Fig. 5<\/b>). A invasividade das c\u00e9lulas de c\u00e2ncer de mama aumentou em fun\u00e7\u00e3o da rigidez da MEC, corroborando dados cl\u00ednicos que sugerem a ocorr\u00eancia preferencial de met\u00e1stases no c\u00e2ncer de mama com maior rigidez tecidual. Observou-se uma mudan\u00e7a para morfologias celulares mais migrat\u00f3rias, associadas \u00e0 met\u00e1stase (Fig. 5, inser\u00e7\u00f5es ampliadas), com o aumento da rigidez da MEC do LunaGel.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-292155c elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"292155c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"355\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1024%2C355&amp;ssl=1\" class=\"attachment-large size-large wp-image-70817\" alt=\"Breast cancer cell behavior on LunaGel ECMs of varying stiffness\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?w=1415&amp;ssl=1 1415w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=300%2C104&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=1024%2C355&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=768%2C266&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=1200%2C416&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=260%2C90&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=50%2C17&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=150%2C52&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"70817\" data-permalink=\"https:\/\/rheolution.com\/pt\/notas-de-aplicacao\/viscoelasticidade-da-gelomica-lunagel-ecm-fotoreticulavel\/attachment\/figure-5_web-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1415%2C491&amp;ssl=1\" data-orig-size=\"1415,491\" data-comments-opened=\"1\" data-image-title=\"Breast cancer cell\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1024%2C355&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-201a0a6 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"201a0a6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 5:  <\/strong>A invasividade das c\u00e9lulas do c\u00e2ncer de mama aumenta com a rigidez da MEC. As imagens mostram esfer\u00f3ides de MDA-MB-231 semeados em MECs de gelatina bovina LunaGel com m\u00f3dulo de armazenamento de cisalhamento de (A) 110 Pa, (B) 220 Pa e (C) 440 Pa, respectivamente, ap\u00f3s 7 dias de cultura. As c\u00e9lulas\/esfer\u00f3ides foram coradas para visualiza\u00e7\u00e3o dos n\u00facleos celulares (azul), da integrina \u03b21 (verde), da membrana celular (laranja) e da actina (magenta).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-298788a elementor-widget elementor-widget-heading\" data-id=\"298788a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Conclus\u00f5es<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a5f8f67 elementor-widget elementor-widget-text-editor\" data-id=\"a5f8f67\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>A evolu\u00e7\u00e3o viscoel\u00e1stica dos produtos ECM LunaGel\u2122 da Gelomics durante a fotoreticula\u00e7\u00e3o foi capturada com sucesso em tempo real utilizando <a href=\"https:\/\/rheolution.com\/pt\/?p=70810\">o ElastoSens\u2122<\/a><a href=\"https:\/\/rheolution.com\/pt\/?p=70810\"> Bio.<\/a> O valor final de G\u2019 foi aproximadamente 8 vezes maior nas formula\u00e7\u00f5es de alta rigidez em compara\u00e7\u00e3o com as de baixa rigidez. As propriedades viscoel\u00e1sticas finais desses hidrog\u00e9is foram facilmente ajustadas variando-se o tempo de exposi\u00e7\u00e3o \u00e0 luz durante a reticula\u00e7\u00e3o (405 nm). A invasividade das c\u00e9lulas de c\u00e2ncer de mama aumentou significativamente com a rigidez da ECM, demonstrando a capacidade do sistema LunaGel\u2122 de imitar fielmente as condi\u00e7\u00f5es fisiol\u00f3gicas e fisiopatol\u00f3gicas dos tecidos.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afd817a elementor-widget elementor-widget-heading\" data-id=\"afd817a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Resumo<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d936ff2 elementor-widget elementor-widget-text-editor\" data-id=\"d936ff2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul class=\"bullets\"><li class=\"bullets\">Os produtos LunaGel\u2122 facilitam o desenvolvimento de hidrog\u00e9is com a rigidez desejada.<\/li><li class=\"bullets\"><a href=\"https:\/\/rheolution.com\/pt\/?p=70810\">O ElastoSens\u2122 Bio<\/a> \u00e9 capaz de aplicar luz (em diferentes comprimentos de onda) durante a medi\u00e7\u00e3o da viscoelasticidade de hidrog\u00e9is, fornecendo a cin\u00e9tica de fotoreticula\u00e7\u00e3o em tempo real.<\/li><li class=\"bullets\">As intensidades de luz e os tempos de exposi\u00e7\u00e3o durante a fotoreticula\u00e7\u00e3o de hidrog\u00e9is podem ser facilmente combinados no <a href=\"https:\/\/rheolution.com\/pt\/?p=70810\">ElastoSens\u2122 Bio<\/a> para atingir a viscoelasticidade desejada para a aplica\u00e7\u00e3o espec\u00edfica.<\/li><li class=\"bullets\">As MECs LunaGel\u2122 facilitam o estudo do comportamento celular em fun\u00e7\u00e3o da rigidez da MEC.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-650fa95 elementor-widget elementor-widget-heading\" data-id=\"650fa95\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Refer\u00eancias<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec1a35b elementor-widget elementor-widget-text-editor\" data-id=\"ec1a35b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>[1] Keely, P., &#038; Nain, A. (2015). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4754037\/\" target=\"_blank\" rel=\"noopener\">Capturing relevant extracellular matrices for investigating cell migration<\/a>. F1000Research, 4.<\/p><p>[2] Wei, S. C., Fattet, L., Tsai, J. H., Guo, Y., Pai, V. H., Majeski, H. E., &#8230; &#038; Yang, J. (2015). <a href=\"https:\/\/www.nature.com\/articles\/ncb3157\" target=\"_blank\" rel=\"noopener\">Matrix stiffness drives epithelial\u2013mesenchymal transition and tumour metastasis through a TWIST1\u2013G3BP2 mechanotransduction pathway<\/a>. Nature cell biology, 17(5), 678-688.<\/p><p>[3] Paszek, M. J., Zahir, N., Johnson, K. R., Lakins, J. N., Rozenberg, G. I., Gefen, A., &#8230; &#038; Weaver, V. M. (2005). <a href=\"https:\/\/www.cell.com\/cancer-cell\/fulltext\/S1535-6108(05)00268-0\" target=\"_blank\" rel=\"noopener\">Tensional homeostasis and the malignant phenotype<\/a>. Cancer cell, 8(3), 241-254.<\/p><p>[4] Caliari, S. R., &#038; Burdick, J. A. (2016). <a href=\"https:\/\/www.nature.com\/articles\/nmeth.3839.pdf\" target=\"_blank\" rel=\"noopener\">A practical guide to hydrogels for cell culture<\/a>. Nature methods, 13(5), 405-414.<\/p><p> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-93251b6 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"93251b6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/wp-content\/uploads\/Viscoelasticity-of-Gelomics-LunaGel-Photocrosslinkable-ECM.pdf\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Acesse a Nota do Aplicativo na \u00edntegra (PDF) <\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-55b7ef2 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"55b7ef2\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper 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amostras<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca42a8b elementor-align-center elementor-widget elementor-widget-button\" data-id=\"ca42a8b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/pt\/contato\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Entre em contato conosco para mais informa\u00e7\u00f5es<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8418b6a elementor-widget elementor-widget-heading\" data-id=\"8418b6a\" data-element_type=\"widget\" data-e-type=\"widget\" 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