{"id":70928,"date":"2026-09-29T14:02:22","date_gmt":"2026-09-29T18:02:22","guid":{"rendered":"https:\/\/rheolution.com\/?p=70928"},"modified":"2026-09-29T14:02:22","modified_gmt":"2026-09-29T18:02:22","slug":"misurare-la-crescita-del-lievito-in-un-bioreattore-tramite-la-torbidita","status":"publish","type":"post","link":"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-crescita-del-lievito-in-un-bioreattore-tramite-la-torbidita\/","title":{"rendered":"Come monitorare la crescita dei lieviti in un bioreattore con TURBIDI.T\u2122?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70928\" class=\"elementor elementor-70928 elementor-29296\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7eee7ee e-flex e-con-boxed e-con e-parent\" data-id=\"7eee7ee\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ea1afcb elementor-widget elementor-widget-heading\" data-id=\"ea1afcb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota applicativa | TURBIDI.T\u2122<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2141c30 elementor-widget elementor-widget-heading\" data-id=\"2141c30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Migliorare il monitoraggio della crescita del lievito grazie alla misurazione della torbidit\u00e0\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e230945 elementor-widget elementor-widget-text-editor\" data-id=\"e230945\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"raw_components--panel--3IcXg inspect_panels--inspectionPanel--3Wboz\" tabindex=\"-1\"><div class=\"inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--propertyRowContent--7Y-mu inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--contentProperty--2Z1QI text--fontPos11--RSei3 text--_fontBase--YWDo0 ellipsis--ellipsisAfter8Lines--2rn32 ellipsis--_ellipsisAfterNLines--26JkZ\"><p>di <a href=\"https:\/\/www.linkedin.com\/in\/gloriapinilla\/\" target=\"_blank\" rel=\"noopener\">Gloria Pinilla<\/a> <span style=\"font-weight: 400;\">e <a href=\"https:\/\/www.linkedin.com\/in\/nora-kasaplar\" target=\"_blank\" rel=\"noopener\">Nora Kasaplar<\/a><\/span>,<\/p><p><span style=\"font-weight: 400;\">Applicazione<\/span><span style=\"font-weight: 400;\"> Scienziati,<\/span> <a href=\"https:\/\/www.linkedin.com\/company\/1168758\" target=\"_blank\" rel=\"noopener\">Rheolution Inc.<\/a><\/p><p>In collaborazione con  <\/p><p><em>Scuola Nazionale Superiore di Tecnologia delle Biomolecole di Bordeaux (ENSTBB).<\/em><\/p><p><span id=\"docs-internal-guid-f0b2c698-7fff-5eae-53f6-94e076f7192c\" style=\"font-weight: normal;\"><span style=\"font-size: 10pt; font-family: Verdana, sans-serif; color: #002060; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space-collapse: preserve;\"><span style=\"border-width: initial; border-style: none; display: inline-block; overflow: hidden; width: 183px; height: 59px;\"><a href=\"https:\/\/enstbb.bordeaux-inp.fr\/fr\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/lh6.googleusercontent.com\/taVq6fxwO3y9M_Onk2MznWVYK4fGUswV4ylXbLTXiz--6Lnb5y_D8UU_0-BYZQ84LvhP4-Wzx6Je0H-TEH850xcAVrlCU1qZoUZuH2Wo37mQLyWOFqe-pn1FNScC6b94yI6cUA5K2fY2Yom1K9-EEAQ\" alt=\"\" width=\"183\" height=\"59\"><\/a><\/span><\/span><\/span><span style=\"letter-spacing: 0px;\"><br><\/span><\/p><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-35f268f elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"35f268f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Sintesi<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb634db elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"bb634db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Il monitoraggio della crescita dei lieviti \u00e8 un processo fondamentale per garantire condizioni ottimali e la produttivit\u00e0 nei processi biotecnologici.  <\/li><li>Le misurazioni basate sulla torbidit\u00e0 si sono affermate come un metodo rapido e non distruttivo per stimare la crescita del lievito e monitorare la densit\u00e0 della coltura nel tempo. <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> (Rheolution, QC, Canada) \u00e8 stato utilizzato per monitorare con precisione la crescita di <i>S. cerevisiae<\/i> nel tempo.<\/li><li>I risultati relativi alla torbidit\u00e0 (850 nm) e all&#8217;assorbanza (600 nm) sono stati messi a confronto, evidenziando andamenti simili e una forte correlazione.<\/li><li><a href=\"https:\/\/rheolution.com\/life-sciences\/products\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> ha dimostrato numerosi vantaggi rispetto allo spettrofotometro per quanto riguarda l\u2019analisi della crescita cellulare e la raccolta dei dati.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9453c51 e-flex e-con-boxed e-con e-parent\" data-id=\"9453c51\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5bdb09e elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5bdb09e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Progressi nel monitoraggio della crescita del lievito: misurazione della torbidit\u00e0<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5965530 elementor-widget elementor-widget-heading\" data-id=\"5965530\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Introduzione<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b5ece66 elementor-widget elementor-widget-text-editor\" data-id=\"b5ece66\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Nel campo della bioingegneria e della biotecnologia, le cellule fungono da catalizzatori per la produzione di diversi prodotti di valore, come biocarburanti, farmaci, anticorpi, enzimi industriali e sostanze chimiche di origine biologica [1]. In questo contesto, il lievito, un microrganismo ampiamente utilizzato, svolge un ruolo fondamentale. Monitorare la crescita del lievito \u00e8 di fondamentale importanza per garantire condizioni ottimali e produttivit\u00e0 nei processi biotecnologici [2].  <\/p><p>Tradizionalmente, l\u2019analisi spettrofotometrica \u00e8 stata usata per valutare la densit\u00e0 ottica a 600 nm (OD600nm) misurando l\u2019assorbimento della luce da parte della coltura di lievito e per monitorare l\u2019evoluzione della torbidit\u00e0 di una popolazione cellulare. Le misurazioni basate sulla torbidit\u00e0 si sono affermate come un approccio rapido e non distruttivo per stimare la crescita del lievito e monitorare la densit\u00e0 della coltura nel tempo. Questa metodologia sfrutta i principi della diffusione della luce, in base ai quali le variazioni nella concentrazione e nelle dimensioni delle cellule influenzano l\u2019intensit\u00e0 della luce diffusa dalla coltura. Quantificando la torbidit\u00e0 risultante, si possono ottenere informazioni preziose sull\u2019andamento della crescita cellulare.   <\/p><p><em>Il Saccharomyces cerevisiae<\/em> (5-10 \u00b5m), noto anche come lievito di panificazione o lievito da birra, occupa un posto di rilievo come organismo modello nel campo della produzione industriale. Ci\u00f2 \u00e8 dovuto alle sue caratteristiche ben definite, alla sua robustezza, all\u2019elevata produttivit\u00e0 e alla sua versatilit\u00e0.   <\/p><p>Questa nota applicativa si concentra sull\u2019uso di <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\">TURBIDI.T\u2122<\/a> (Rheolution, QC, Canada) per monitorare la crescita di <em>S. cerevisiae<\/em> nel tempo. I risultati ottenuti vengono confrontati e messi in relazione con le misurazioni di OD a 600 nm effettuate con uno spettrofotometro standard. Analizzando questo confronto, si possono ricavare informazioni preziose per comprendere meglio l&#8217;efficacia e l&#8217;affidabilit\u00e0 di <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\">TURBIDI.T\u2122<\/a> come strumento di monitoraggio della crescita del lievito.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-62f3970 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"62f3970\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Monitoraggio della crescita del lievito tramite la misurazione della torbidit\u00e0: un caso di studio su S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-978e221 elementor-widget elementor-widget-heading\" data-id=\"978e221\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Materiali e metodi<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-299f474 elementor-widget elementor-widget-text-editor\" data-id=\"299f474\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00c8 stata preparata una precultura di lievito da 100 mL in condizioni sterili, inoculando colonie <em>di S. cerevisiae<\/em> provenienti da una coltura solida su una piastra di Petri, seguita da una crescita notturna. Contemporaneamente, \u00e8 stato preparato un bioreattore da 3 L (<a href=\"https:\/\/www.sartorius.com\/en\/products\/fermentation-bioreactors\/benchtop-bioreactors\/biostat-b?_gl=1*dzzxof*_up*MQ..&#038;gclid=CjwKCAjw-7OlBhB8EiwAnoOEkyL2kNBymV2yGELc9KSmyLWW_BODZo5jZrEa5-9mE9bDhvznnIe9FBoCDHsQAvD_BwE\">BIOSTAT B; Sartorius Stedim Biotech, G\u00f6ttingen, Germania<\/a>) contenente terreno di coltura sintetico sterile [3] \u00e8 stato preparato per la sterilizzazione notturna in autoclave. Il terreno di coltura sintetico era composto da vari sali inorganici ((NH\u2084)\u2082SO\u2084, KH\u2082PO\u2084, MgSO\u2084), vitamine, oligoelementi e glucosio. Dopo la sterilizzazione, le sonde di pH e pO\u2082, cos\u00ec come la pompa di alimentazione del glucosio, sono state calibrate seguendo le istruzioni fornite dal software del BIOSTAT B (<strong>Figura 1<\/strong>).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8c2bea5 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"8c2bea5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1604\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" class=\"attachment-full size-full wp-image-29333\" alt=\"Yeast bioreactor in sterile conditions.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?w=1604&amp;ssl=1 1604w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=300%2C192&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1024%2C654&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=768%2C490&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1536%2C981&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1200%2C766&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=229%2C146&amp;ssl=1 229w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=50%2C32&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=117%2C75&amp;ssl=1 117w\" sizes=\"(max-width:767px) 700px, (max-width:1604px) 100vw, 1604px\" data-attachment-id=\"29333\" data-permalink=\"https:\/\/rheolution.com\/it\/fig1_biostat-software_merged\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" data-orig-size=\"1604,1024\" data-comments-opened=\"1\" data-image-title=\"Yeast bioreactor\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1024%2C654&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f611ec3 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f611ec3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 1: <\/b>Configurazione <b>del bioreattore <\/b>per la coltura del lievito in condizioni sterili.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9aba0ca elementor-widget elementor-widget-text-editor\" data-id=\"9aba0ca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Per calibrare il <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, prima di eseguire i test sono stati utilizzati standard di formazina. L\u2019emissione e la ricezione della luce sono state attivate utilizzando rispettivamente le cartucce Emitt.805 (lunghezza d\u2019onda di 850 nm) e Receiv.ViS (lunghezza d\u2019onda compresa tra 400 nm e 1000 nm). Abbiamo scelto di utilizzare la cartuccia da 850 nm poich\u00e9 l\u2019effetto dell\u2019assorbimento della luce era minore. Lo spettrofotometro (<a href=\"https:\/\/www.safas.com\/?SAFAS-UVmc1-spectrophotometer-the-price-and-use-of-a-single-beam-with-double-beam-quality&#038;lang=en\" target=\"_blank\" rel=\"noopener\">SAFAS UVmc, SAFAS, Monaco<\/a>) \u00e8 stato calibrato con una fiala di acqua distillata deionizzata (ddH\u2082O) prima di ogni misurazione.  <\/p><p>I lieviti precoltivati sono stati inoculati nel bioreattore a una densit\u00e0 di 220 FTU (0,21 uOD). Successivamente, la crescita del lievito \u00e8 stata monitorata prelevando aliquote di coltura ogni ora per 8 ore. Le misurazioni della torbidit\u00e0 a 850 nm (espressa in FTU) e dell\u2019assorbanza (espressa in uOD) sono state effettuate in triplicato per ciascun intervallo di tempo (n=3). Per le misurazioni spettrofotometriche, l\u2019aliquota della coltura di lieviti \u00e8 stata diluita in rapporto 1:100, mentre per quelle turbidimetriche non \u00e8 stata necessaria alcuna diluizione e le aliquote sono state analizzate direttamente. I risultati sono presentati come media \u00b1 deviazione standard.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2ad55a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"b2ad55a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Analisi delle dinamiche di crescita del lievito: misurazioni della torbidit\u00e0 rispetto all\u2019assorbanza nelle colture di S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aadb984 elementor-widget elementor-widget-heading\" data-id=\"aadb984\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Risultati e discussione<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99aa2a5 elementor-widget elementor-widget-text-editor\" data-id=\"99aa2a5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>La figura 2<\/b> mostra l\u2019andamento della crescita del lievito nel tempo, con un notevole aumento esponenziale sia in unit\u00e0 di torbidit\u00e0 (FTU) che in unit\u00e0 di assorbanza (uOD) dopo un breve periodo di latenza di 2 ore. Durante la fase di latenza, le cellule di lievito attraversano un periodo di adattamento, adeguandosi gradualmente all\u2019ambiente circostante. Al termine di questa fase, la curva decolla, dimostrando la proliferazione continua delle cellule di lievito. Questa fase di crescita esponenziale indica la presenza di condizioni ottimali per la crescita del lievito, in cui fattori come la disponibilit\u00e0 di nutrienti e gli stimoli ambientali favoriscono la divisione cellulare attiva e l\u2019attivit\u00e0 metabolica.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bd7401d elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"bd7401d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"2827\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29324\" alt=\"Turbidity trend of S. cerevisiae culture over 8h, 850nm vs. OD600nm comparison graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2827&amp;ssl=1 2827w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=300%2C170&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1024%2C580&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=768%2C435&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1536%2C870&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=2048%2C1160&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1200%2C680&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=258%2C146&amp;ssl=1 258w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=50%2C28&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=132%2C75&amp;ssl=1 132w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2440&amp;ssl=1 2440w\" sizes=\"(max-width:767px) 700px, (max-width:2827px) 100vw, 2827px\" data-attachment-id=\"29324\" data-permalink=\"https:\/\/rheolution.com\/it\/fig2_cell-grow-evolution\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" data-orig-size=\"2827,1601\" data-comments-opened=\"1\" data-image-title=\"Turbidity trend of S. cerevisiae\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=1024%2C580&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7801f2e elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"7801f2e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: bold; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">Figura 2:  <\/span><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\">Configurazione del bioreattore per la coltura del lievito in condizioni sterili. Andamento della torbidit\u00e0 di   <\/span><i style=\"font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">S. cerevisiae<\/i><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\"> coltura per 8 ore e confronto tra la torbidit\u00e0 a 850 nm e l&#8217;OD<\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\"><span style=\"font-size: 0.6em; vertical-align: sub;\">600 nm<\/span><\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-52474e0 elementor-widget elementor-widget-text-editor\" data-id=\"52474e0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Le misurazioni della torbidit\u00e0 ottenute con <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\">TURBIDI.T\u2122<\/a> (rappresentate sull\u2019asse Y di sinistra) coincidono perfettamente con i valori di assorbanza ottenuti con uno spettrofotometro standard (visualizzati sull\u2019asse Y di destra), dato che entrambe le curve mostrano un andamento simile. Le deviazioni standard erano tutte molto basse (barre di errore incluse nel grafico), a dimostrazione dell&#8217;elevata precisione delle misurazioni (variabilit\u00e0 inferiore all&#8217;1%). <\/p><p>In linea con le osservazioni della <strong>Figura 2<\/strong>, c\u2019\u00e8 una correlazione lineare positiva tra la torbidit\u00e0 e le misurazioni dell\u2019OD a 600 nm, con un coefficiente di determinazione (R\u00b2) davvero notevole pari a 0,998 (<strong>Figura 3<\/strong>). Questo elevato valore di R\u00b2 indica una relazione forte e affidabile tra la torbidit\u00e0 e l\u2019OD a 600 nm, suggerendo che le misurazioni della torbidit\u00e0 possano effettivamente sostituire quelle basate sull\u2019assorbanza nel monitoraggio della crescita del lievito. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-97440cd elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"97440cd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2001\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29326\" alt=\"FTU (850 nm) vs. uOD (600 nm) Correlation in Cell Culture Growth Graph.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?w=2001&amp;ssl=1 2001w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=300%2C240&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1024%2C819&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=768%2C614&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1536%2C1229&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1200%2C960&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=182%2C146&amp;ssl=1 182w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=50%2C40&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=94%2C75&amp;ssl=1 94w\" sizes=\"(max-width:767px) 700px, (max-width:2001px) 100vw, 2001px\" data-attachment-id=\"29326\" data-permalink=\"https:\/\/rheolution.com\/it\/fig3_correlation-between-ftu-and-uod\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" data-orig-size=\"2001,1601\" data-comments-opened=\"1\" data-image-title=\"Correlation in Cell Culture Growth\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=1024%2C819&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ccd96b elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"1ccd96b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 3: Correlazione <\/b>tra FTU (850 nm) e uOD (600 nm) nella misurazione della crescita delle colture cellulari.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8a7ac8d elementor-widget elementor-widget-text-editor\" data-id=\"8a7ac8d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La stretta corrispondenza tra le misurazioni della torbidit\u00e0 e dell\u2019assorbanza, insieme all\u2019elevato coefficiente di correlazione, evidenzia l\u2019efficacia e l\u2019accuratezza delle misurazioni basate sulla torbidit\u00e0 effettuate con <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> per monitorare le dinamiche di crescita del lievito.<\/p><p>Inoltre, la possibilit\u00e0 di ottenere dati affidabili sulla crescita senza dover diluire i campioni, come invece richiede l&#8217;analisi spettrofotometrica tradizionale, sottolinea ulteriormente i vantaggi offerti dall&#8217;approccio basato sulla torbidit\u00e0. Nel complesso, questi risultati dimostrano il potenziale di <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> come strumento prezioso per il monitoraggio preciso ed efficiente della crescita del lievito nei bioprocessi, contribuendo ai progressi nella ricerca e nelle applicazioni biotecnologiche.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19ccca4 elementor-widget elementor-widget-heading\" data-id=\"19ccca4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Conclusioni e prospettive<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a93e4fa elementor-widget elementor-widget-text-editor\" data-id=\"a93e4fa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Il sistema <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> si \u00e8 dimostrato uno strumento potente e preciso per monitorare la crescita e la cinetica delle colture cellulari nelle colture di lievito.  <\/li><li>Il confronto tra la torbidit\u00e0 e l\u2019OD a 600 nm mostra una forte relazione lineare, confermando la torbidit\u00e0 come un\u2019alternativa precisa alle misurazioni basate sull\u2019assorbanza. Questo mette in evidenza l\u2019efficacia della torbidit\u00e0 nel valutare con precisione le dinamiche di crescita del lievito. <\/li><li><a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> offre diversi vantaggi rispetto all\u2019analisi spettrofotometrica. L\u2019eliminazione della diluizione del campione, grazie all\u2019ampio intervallo di valori di torbidit\u00e0 rilevati dallo strumento <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, riduce gli errori di misurazione e fa risparmiare tempo prezioso. Inoltre, il tablet portatile permette di raccogliere e archiviare facilmente i dati, migliorando la praticit\u00e0 e l\u2019accessibilit\u00e0.    <\/li><li>La versatilit\u00e0 del sistema <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> permette di utilizzare diverse fiale per le misurazioni in base alle diverse configurazioni sperimentali.<\/li><li>La scalabilit\u00e0 del sistema <a href=\"https:\/\/rheolution.com\/it\/prodotti\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> ne aumenta ulteriormente il valore. Collegando pi\u00f9 unit\u00e0 a un unico tablet di comando si migliora l\u2019efficienza dei test, consentendo misurazioni simultanee in esperimenti su larga scala. <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d297cde elementor-align-center elementor-widget elementor-widget-button\" data-id=\"d297cde\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/wp-content\/uploads\/Microbiology_turbidity-1.pdf\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Accedi alla versione completa della nota sull'app (PDF) <\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee666c7 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"ee666c7\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-c97f30c swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"TURBIDI.T\u2122: Modern IoT-Enabled, Modular Turbidimeter.\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div><div class=\"elementor-repeater-item-1ae3858 swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"TURBIDI.T\u2122 with Cartridges: Modern IoT-Enabled Modular Turbidimeter.\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div><div class=\"elementor-repeater-item-909265f swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Contattaci per ricevere un preventivo o per saperne di pi\u00f9<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69d0943 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"69d0943\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/it\/contatti\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Contattaci per saperne di pi\u00f9<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-469fdb5 elementor-widget elementor-widget-heading\" data-id=\"469fdb5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Riferimenti<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-685d6db elementor-widget elementor-widget-text-editor\" data-id=\"685d6db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>[1]  <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S095816692100152X\" target=\"_blank\" rel=\"noopener\">Reardon, K. F. (2021). Practical monitoring technologies for cells and substrates in biomanufacturing. Current Opinion in Biotechnology, 71, 225-230.  <\/a><\/p><p>[2]  <a href=\"https:\/\/link.springer.com\/article\/10.1186\/s12934-021-01617-z\" target=\"_blank\" rel=\"noopener\">Madhavan, A., Arun, K. B., Sindhu, R., Krishnamoorthy, J., Reshmy, R., Sirohi, R., &#8230; &#038; Binod, P. (2021). Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up. Microbial cell factories, 20(1), 124.  <\/a><\/p><p>[3]<a href=\"https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/\" target=\"_blank\" rel=\"noopener\">Tankeshwar Acharya (2021). Bacterial Culture Media: Classification, Types, Uses. Retrieved from https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/.  <\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec9a286 elementor-widget elementor-widget-heading\" data-id=\"ec9a286\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Articoli correlati<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca12d3f premium-blog-cta-full-yes premium-floating-effects-yes premium-disable-fe-yes elementor-widget__width-initial premium-blog-align-left elementor-widget elementor-widget-premium-addon-blog\" data-id=\"ca12d3f\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;force_height&quot;:&quot;true&quot;,&quot;premium_blog_grid&quot;:&quot;yes&quot;,&quot;premium_blog_layout&quot;:&quot;even&quot;,&quot;premium_blog_columns_number&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_tablet&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_mobile&quot;:&quot;100%&quot;,&quot;scroll_to_offset&quot;:&quot;yes&quot;,&quot;premium_fe_trigger&quot;:&quot;load&quot;,&quot;premium_fe_direction&quot;:&quot;alternate&quot;,&quot;premium_fe_loop&quot;:&quot;default&quot;,&quot;premium_fe_easing&quot;:&quot;easeInOutSine&quot;}\" data-widget_type=\"premium-addon-blog.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t\n\n\t\t\t\t<div class=\"premium-blog-wrap  premium-blog-even\" data-page=\"70928\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65479\" alt=\"Blog Post Image: Methods to Quantify Cell Concentration Using TURBIDI.T\u2122 Technology.\" data-attachment-id=\"65479\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/quantificare-la-concentrazione-cellulare-con-il-metodo-turbidi-t\/attachment\/quantifying-cell-concentration-in-culture-media-with-turbidi-t-header-3-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Methods to Quantify Cell Concentration\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1024%2C384&amp;ssl=1\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/it\/note-applicative\/quantificare-la-concentrazione-cellulare-con-il-metodo-turbidi-t\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Come si quantifica la concentrazione cellulare con TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/it\/note-applicative\/quantificare-la-concentrazione-cellulare-con-il-metodo-turbidi-t\/\" target=\"_blank\">\n\t\t\t\tCome si quantifica la concentrazione cellulare con TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">La crescente richiesta di metodi pi\u00f9 rapidi per la quantificazione cellulare va oltre le tradizionali camere di conteggio, spingendo verso soluzioni innovative che facilitano un conteggio accurato riducendo i tempi. La torbidit\u00e0, che riflette l\u2019opacit\u00e0 della soluzione in presenza di particelle che diffondono la luce, si rivela un mezzo efficace per accelerare la quantificazione cellulare.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/it\/note-applicative\/quantificare-la-concentrazione-cellulare-con-il-metodo-turbidi-t\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leggi di pi\u00f9 \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65591\" alt=\"Measuring liquid particle size with TURBIDI.T\u2122.\" data-attachment-id=\"65591\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-dimensione-delle-particelle-in-un-campione-liquido\/attachment\/web_banner_size-4-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Measuring liquid particle\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1024%2C384&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?w=1200&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=300%2C113&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=1024%2C384&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=768%2C288&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=260%2C98&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=50%2C19&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=150%2C56&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-dimensione-delle-particelle-in-un-campione-liquido\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Come si misura la dimensione delle particelle in un campione liquido con TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-dimensione-delle-particelle-in-un-campione-liquido\/\" target=\"_blank\">\n\t\t\t\tCome si misura la dimensione delle particelle in un campione liquido con TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">La determinazione delle dimensioni delle particelle \u00e8 importante in molti campi, come le scienze della vita (nanomedicina, somministrazione di farmaci, ingegneria tissutale, bioanalisi), la chimica, le scienze ambientali e l\u2019industria. La turbidimetria \u00e8 un metodo veloce e non distruttivo per stimare le dimensioni delle particelle seguendo una procedura semplice da seguire.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-dimensione-delle-particelle-in-un-campione-liquido\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leggi di pi\u00f9 \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Nel campo della bioingegneria e della biotecnologia, le cellule fungono da catalizzatori per la produzione di diversi prodotti di valore, come biocarburanti, farmaci, anticorpi, enzimi industriali e sostanze chimiche di origine biologica [1]. In questo contesto, il lievito, un microrganismo ampiamente utilizzato, svolge un ruolo fondamentale. Monitorare la crescita del lievito \u00e8 fondamentale per garantire condizioni ottimali e la massima produttivit\u00e0 nei processi biotecnologici [2]. Tradizionalmente, si ricorre all\u2019analisi spettrofotometrica per valutare la densit\u00e0 ottica a 600 nm (OD600nm), misurando l\u2019assorbimento della luce da parte del lievito&#8230;   <\/p>\n","protected":false},"author":195401338,"featured_media":70929,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[289,317,312],"tags":[123,148,124,121,137],"class_list":["post-70928","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-note-applicative","category-turbidi-t-life-sciences","category-turbidit-agrifood","tag-agri-food","tag-comparison-of-technologies","tag-life-sciences","tag-microbiology","tag-agri-food-turbidity"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Monitoraggio della Crescita del Lievito: Torbidit\u00e0 nei Bioreattori<\/title>\n<meta name=\"description\" content=\"Usa gli strumenti ottici IoT di Rheolution per monitorare con precisione le colture cellulari e la crescita batterica grazie a misurazioni avanzate della torbidit\u00e0.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/rheolution.com\/it\/note-applicative\/misurare-la-crescita-del-lievito-in-un-bioreattore-tramite-la-torbidita\/\" \/>\n<meta property=\"og:locale\" content=\"it_IT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Monitoraggio della Crescita del Lievito: Torbidit\u00e0 nei Bioreattori\" \/>\n<meta property=\"og:description\" content=\"Usa gli strumenti ottici IoT di Rheolution 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