{"id":70797,"date":"2026-09-29T12:04:10","date_gmt":"2026-09-29T16:04:10","guid":{"rendered":"https:\/\/rheolution.com\/?p=70797"},"modified":"2026-09-29T12:04:10","modified_gmt":"2026-09-29T16:04:10","slug":"viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile","status":"publish","type":"post","link":"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/","title":{"rendered":"Regolazione della rigidit\u00e0 degli idrogel a base di ECM"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70797\" class=\"elementor elementor-70797 elementor-19680\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9926633 e-flex e-con-boxed e-con e-parent\" data-id=\"9926633\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2aa8b2e elementor-widget elementor-widget-heading\" data-id=\"2aa8b2e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota applicativa | ElastoSens\u2122 Bio<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b490e4b elementor-widget elementor-widget-heading\" data-id=\"b490e4b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Regolazione della rigidit\u00e0 degli idrogel ECM con ElastoSens\u2122 Bio<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ed01f46 elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"ed01f46\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">In collaborazione con<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-989a838 elementor-widget elementor-widget-image\" data-id=\"989a838\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/www.gelomics.com\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"80\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=300%2C80&amp;ssl=1\" class=\"attachment-medium size-medium wp-image-70798\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=9178&amp;ssl=1 9178w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=300%2C80&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1024%2C272&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=768%2C204&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1536%2C407&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=2048%2C543&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=1200%2C318&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=260%2C69&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=50%2C13&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?resize=150%2C40&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 300px, 300px\" data-attachment-id=\"70798\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/gelomics_logo_horizontal_with_claim_full-colour_181002-01-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=9178%2C2434&amp;ssl=1\" data-orig-size=\"9178,2434\" data-comments-opened=\"1\" data-image-title=\"Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Gelomics_Logo_Horizontal_with_Claim_Full-colour_181002-01.png?fit=1024%2C272&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9026f26 elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"9026f26\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Avviso<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3db1a2 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"d3db1a2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Questo studio \u00e8 stato realizzato in collaborazione tra <a href=\"https:\/\/rheolution.com\/life-sciences\/\">Rheolution<\/a> (QC, Canada) e <a href=\"https:\/\/www.gelomics.com\/hydrogels\">Gelomics<\/a> (QLD, Australia).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3ef1a56 elementor-widget elementor-widget-text-editor\" data-id=\"3ef1a56\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"raw_components--panel--3IcXg inspect_panels--inspectionPanel--3Wboz\" tabindex=\"-1\"><div class=\"inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--propertyRowContent--7Y-mu inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--contentProperty--2Z1QI text--fontPos11--RSei3 text--_fontBase--YWDo0 ellipsis--ellipsisAfter8Lines--2rn32 ellipsis--_ellipsisAfterNLines--26JkZ\"><p>di <a href=\"https:\/\/www.linkedin.com\/in\/gloriapinilla\/\" target=\"_blank\" rel=\"noopener\">Gloria Pinilla, M.Sc.<\/a>, Specialista senior per le applicazioni,<br\/>Rheolution Inc.<\/p><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-29c9a4e elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"29c9a4e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Sintesi<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23a78fe elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"23a78fe\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>La rigidit\u00e0 iniziale dei sistemi di coltura 3D pu\u00f2 essere modificata per studiare i processi fisiologici e patologici in vitro.<\/li><li>Gli idrogel fotocrosslinkabili sono ottimi candidati per i sistemi di coltura 3D grazie alla loro somiglianza con la matrice extracellulare naturale e alla facilit\u00e0 con cui si pu\u00f2 regolare la loro rigidit\u00e0 finale.<\/li><li>I prodotti LunaGel\u2122 a matrice extracellulare di Gelomics sono stati reticolati con successo tramite luce visibile e la loro cinetica di reticolazione \u00e8 stata misurata in tempo reale con <a href=\"https:\/\/rheolution.com\/it\/prodotti\/elastosens-bio\/strumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a>.<\/li><li>Le reazioni di fotoreticolazione si sono svolte rapidamente sia nel kit LunaGel\u2122 a bassa rigidit\u00e0 che in quello ad alta rigidit\u00e0.<\/li><li>I kit LunaGel\u2122 ad alta rigidit\u00e0 hanno mostrato una rigidit\u00e0 maggiore rispetto a quelli a bassa rigidit\u00e0.<\/li><li>La rigidit\u00e0 delle matrici LunaGel\u2122 \u00e8 aumentata con l&#8217;aumentare della durata della reticolazione fotochimica.<\/li><li>L&#8217;invasivit\u00e0 delle cellule tumorali al seno \u00e8 aumentata in modo significativo con l&#8217;aumentare della rigidit\u00e0 della matrice extracellulare (ECM).<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a11b72f e-flex e-con-boxed e-con e-parent\" data-id=\"a11b72f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ff67a5f elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"ff67a5f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Il ruolo della rigidit\u00e0 dell'idrogel nel comportamento delle cellule\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ddf558e elementor-widget elementor-widget-heading\" data-id=\"ddf558e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Introduzione<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dcef2a1 elementor-widget elementor-widget-text-editor\" data-id=\"dcef2a1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Tutte le cellule del corpo umano sono esposte a forze meccaniche che regolano la loro funzione e lo sviluppo dei tessuti, e ogni tipo di cellula si adatta in modo specifico alle propriet\u00e0 meccaniche del tessuto in cui si trova. Le propriet\u00e0 della matrice dei tessuti umani possono anche cambiare in presenza di una malattia e, a loro volta, favorirne la progressione. Ad esempio, la crescita, la sopravvivenza, la differenziazione e la morfogenesi delle cellule epiteliali mammarie normali sono ben supportate dall\u2019interazione con una matrice morbida, simile alla rigidit\u00e0 del tessuto mammario sano. A seguito della trasformazione che si verifica nel cancro al seno, per\u00f2, il tessuto diventa progressivamente pi\u00f9 rigido [1] e le cellule tumorali diventano significativamente pi\u00f9 contrattili e iper-reattive agli stimoli meccanici della matrice, favorendo in ultima analisi la transizione epiteliale-mesenchimale (EMT) e le metastasi [2,3]. Lo studio dei meccanismi alla base della complessa interazione tra le propriet\u00e0 del substrato e il comportamento cellulare richiede metodologie avanzate di coltura cellulare in grado di superare i limiti associati agli approcci tradizionali [4].    <\/p><p>Le matrici extracellulari (ECM) LunaGel\u2122 di Gelomics sono idrogel fotoreticolabili che offrono un sistema di coltura cellulare 3D controllato per studiare i processi patologici e fisiologici <i>in vitro<\/i>. Basate su componenti delle ECM come il collagene di tipo I, III, IV e V, oltre che sulle glicoproteine e sui proteoglicani del tessuto connettivo, queste ECM riproducono fedelmente la matrice extracellulare naturale (ECM) che circonda le cellule nel corpo umano e facilitano l\u2019adesione, la proliferazione, la differenziazione, la migrazione delle cellule e la degradazione proteolitica dell\u2019ECM. Inoltre, il processo di reticolazione fotoindotta permette agli scienziati di avere un maggiore controllo sulla rigidit\u00e0 finale. Basta variare la durata della reticolazione per regolare con precisione la rigidit\u00e0 del gel, in modo da riprodurre tessuti morbidi e duri come il cervello, il fegato, il seno, la cartilagine e altri, sia in condizioni sane che patologiche. Per questi motivi, sono substrati ideali per la coltura e la differenziazione di un\u2019ampia gamma di tipi cellulari e facilitano lo studio dei cambiamenti nel comportamento cellulare indotti dalla rigidit\u00e0 della matrice.      <\/p><p><a href=\"https:\/\/rheolution.com\/it\/prodotti\/elastosens-bio\/strumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a> misura senza contatto le propriet\u00e0 viscoelastiche dei materiali morbidi. Applicando lievi vibrazioni al campione, lo strumento \u00e8 in grado di registrare la cinetica di fotoreticolazione alla lunghezza d\u2019onda desiderata. In questa nota applicativa, gli ECM LunaGel\u2122 a base di pelle suina modificata chimicamente e gelatina di ossa bovine sono stati entrambi sottoposti a fotoreticolazione, mentre la loro cinetica di reticolazione \u00e8 stata valutata in tempo reale con <a href=\"https:\/\/rheolution.com\/it\/prodotti\/elastosens-bio\/strumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a>. Per verificare se le ECM LunaGel\u2122 fossero adatte a riprodurre i cambiamenti fisiopatologici nella rigidit\u00e0 dei tessuti e i relativi effetti sulla progressione del cancro, sono stati coltivati sferoidi di cancro al seno su substrati di diversa rigidit\u00e0 e ne sono state osservate le morfologie.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cc16549 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"cc16549\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Fotoreticolazione degli idrogel LunaGel\u2122 con la tecnologia Gelomics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cae21bc elementor-widget elementor-widget-heading\" data-id=\"cae21bc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Materiali e metodi<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dfb4356 elementor-widget elementor-widget-text-editor\" data-id=\"dfb4356\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Gli ECM LunaGel\u2122 a base di gelatina di pelle suina modificata chimicamente, tipo A (<a href=\"https:\/\/www.gelomics.com\/product-page\/porcine-skin-gelatin\">LunaGel\u2122 \u2013 Gelatina di pelle suina \u2013 bassa e alta rigidit\u00e0, Gelomics<\/a>) e gelatina di osso bovino, tipo B (<a href=\"https:\/\/www.gelomics.com\/product-page\/bovine-bone-gelatin\">LunaGel\u2122 \u2013 Gelatina di osso bovino \u2013 bassa e alta rigidit\u00e0, Gelomics<\/a>) sono stati preparati seguendo le istruzioni del produttore. In breve, le fiale di fotoiniziatore sono state sciolte in PBS e mescolate con le soluzioni di ECM fotoreticolabili a 37 \u00b0C. Sono stati pipettati 2 mL dei prodotti nel portacampioni <a href=\"https:\/\/rheolution.com\/it\/prodotti\/elastosens-bio\/strumento\/\" target=\"_blank\" rel=\"noopener\">dell\u2019ElastoSens\u2122 Bio<\/a> e il test di viscoelasticit\u00e0 \u00e8 stato avviato immediatamente a 37 \u00b0C utilizzando le seguenti sequenze di misurazione:  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3665319 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"3665319\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"304\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=1024%2C304&amp;ssl=1\" class=\"attachment-large size-large wp-image-70807\" alt=\"Table showing crossover of G\u2019 and G\u2019\u2019 indicating initial crosslinking in ElastoSens\u2122 Bio\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?w=2778&amp;ssl=1 2778w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=300%2C89&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1024%2C304&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=768%2C228&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1536%2C456&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=2048%2C607&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=1200%2C356&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=260%2C77&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=50%2C15&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?resize=150%2C44&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?w=2440&amp;ssl=1 2440w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"70807\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/screen-shot-2021-11-18-at-10-26-59-am-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=2778%2C824&amp;ssl=1\" data-orig-size=\"2778,824\" data-comments-opened=\"1\" data-image-title=\"crossover of G\u2019 and G\u2019\u2019\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Screen-Shot-2021-11-18-at-10.26.59-AM.png?fit=1024%2C304&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-10b03f2 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"10b03f2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Il modulo elastico di taglio (G\u2019) e tan(\u03b4) sono stati ricavati direttamente dall\u2019ElastoSens\u2122 Bio. Il tempo iniziale di reticolazione \u00e8 stato determinato in base all\u2019intersezione tra G\u2019 e G\u2019\u2019 (tan(\u03b4) = 1). <\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0205664 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"0205664\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">La rigidit\u00e0 dell'idrogel influenza il comportamento delle cellule<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d2fcdb2 elementor-widget elementor-widget-heading\" data-id=\"d2fcdb2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Risultati e discussione<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8b37e89 elementor-widget elementor-widget-text-editor\" data-id=\"8b37e89\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Gli ECM LunaGel\u2122 fotocrosslinkabili sono stati innanzitutto valutati in termini di propriet\u00e0 viscoelastiche, che possono essere descritte dal modulo di immagazzinamento al taglio (G\u2019 \u2013 parte elastica del comportamento viscoelastico) e dal modulo di perdita al taglio (G\u2019\u2019 \u2013 parte viscosa del comportamento viscoelastico). Di solito si calcola anche il rapporto tra G\u2019\u2019 e G\u2019 (fattore di smorzamento o tan(\u03b4)) per capire quale comportamento sia predominante.   <\/p><p><b>La fig. 1<\/b> mostra l\u2019evoluzione del modulo di conservazione al taglio (G\u2019) in funzione del tempo di reticolazione per le ECM LunaGel\u2122 fotocrosslinkabili a bassa e alta rigidit\u00e0, di origine suina (a sinistra) e bovina (a destra). Le misurazioni sono state effettuate senza esposizione alla luce durante i primi 2 minuti del test. La luce \u00e8 stata poi programmata per essere accesa dallo strumento <a href=\"https:\/\/rheolution.com\/it\/prodotti\/elastosens-bio\/strumento\/\" target=\"_blank\" rel=\"noopener\">ElastoSens\u2122 Bio<\/a> dopo 2 minuti e il suo effetto \u00e8 stato chiaramente osservato con l\u2019aumento di G\u2019 dopo questo momento fino a 12 minuti (quando la luce \u00e8 stata spenta). Le misurazioni sono proseguite per circa 15 minuti per determinare le propriet\u00e0 viscoelastiche finali del gel. I campioni risultavano reticolati in modo omogeneo al termine del test (<b>Fig. 2<\/b>).    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e04bd87 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"e04bd87\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"861\" height=\"426\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" class=\"attachment-large size-large wp-image-70800\" alt=\"G\u2019 over time for LunaGel\u2122 ECMs: porcine (left) &amp; bovine (right)\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?w=861&amp;ssl=1 861w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=300%2C148&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=768%2C380&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=260%2C129&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=50%2C25&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?resize=150%2C75&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:861px) 100vw, 861px\" data-attachment-id=\"70800\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/fig1_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" data-orig-size=\"861,426\" data-comments-opened=\"1\" data-image-title=\"G\u2019 over time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig1_Gelomics_ECM.jpg?fit=861%2C426&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3a0f6a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"d3a0f6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 1:  <\/strong>Modulo di conservazione al taglio (G\u2019, Pa) in funzione del tempo per i prodotti ECM LunaGel\u2122 suini (a sinistra) e bovini (a destra).<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f58da9e elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"f58da9e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"825\" height=\"371\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" class=\"attachment-large size-large wp-image-70801\" alt=\"Crosslinked LunaGel\u2122 sample post-testing\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?w=825&amp;ssl=1 825w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=300%2C135&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=768%2C345&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=260%2C117&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=50%2C22&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?resize=150%2C67&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:825px) 100vw, 825px\" data-attachment-id=\"70801\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/image-2-6\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" data-orig-size=\"825,371\" data-comments-opened=\"1\" data-image-title=\"Crosslinked LunaGel\u2122\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Image-2.jpg?fit=825%2C371&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b3cbd35 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"b3cbd35\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 2:  <\/strong>Esempio rappresentativo di un campione di LunaGel\u2122 dopo il test.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ade62a3 elementor-widget elementor-widget-text-editor\" data-id=\"ade62a3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La reticolazione fotoindotta \u00e8 iniziata dopo circa 0,5 min di esposizione alla luce per gli idrogel ad alta rigidit\u00e0 e dopo circa 1,5 min per quelli a bassa rigidit\u00e0 (<b>Fig. 3<\/b>). Il valore finale di G\u2019 era circa 8 volte pi\u00f9 alto per le formulazioni ad alta rigidit\u00e0 rispetto a quelle a bassa rigidit\u00e0. Le formulazioni a bassa rigidit\u00e0 hanno formato gel morbidi con un G\u2019 di ~ 550 Pa, mentre gli idrogel ad alta rigidit\u00e0 hanno raggiunto ~ 4 500 Pa e non \u00e8 stata riscontrata alcuna differenza statistica tra le fonti suine e quelle bovine. Tuttavia, sono state osservate differenze significative tra le formulazioni a bassa e alta rigidit\u00e0 per entrambi i tipi di gelatina. Infatti, i prodotti LunaGel\u2122 a bassa e alta rigidit\u00e0 contengono concentrazioni diverse dei componenti della gelatina modificata per consentire un controllo preciso su un ampio intervallo di rigidit\u00e0. Il valore finale di tan(\u03b4) \u00e8 risultato sempre inferiore a 0,1, a dimostrazione del fatto che in questi idrogel il comportamento elastico (correlato al modulo di accumulo di taglio, G\u2019\u2019) \u00e8 molto pi\u00f9 predominante rispetto al comportamento viscoso (correlato al modulo di perdita di taglio, G\u2019\u2019).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f87206d elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"f87206d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1020\" height=\"401\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" class=\"attachment-large size-large wp-image-70802\" alt=\"Initial crosslinking time and final G\u2019\/tan(\u03b4) of LunaGel\u2122 gels\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?w=1020&amp;ssl=1 1020w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=300%2C118&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=768%2C302&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=260%2C102&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=50%2C20&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?resize=150%2C59&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1020px) 100vw, 1020px\" data-attachment-id=\"70802\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/fig3_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" data-orig-size=\"1020,401\" data-comments-opened=\"1\" data-image-title=\"crosslinking time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig3_Gelomics_ECM.jpg?fit=1020%2C401&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a66f9b4 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"a66f9b4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 3:<\/strong>  Tempo iniziale di reticolazione (min), G\u2019 finale (Pa) e tan(\u03b4) finale dei gel fotoreticolati.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df11603 elementor-widget elementor-widget-text-editor\" data-id=\"df11603\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Sia per le formulazioni a bassa che ad alta rigidit\u00e0, la durata dell\u2019esposizione alla luce pu\u00f2 essere variata per raggiungere i valori di rigidit\u00e0 desiderati, inferiori o uguali al valore finale di G\u2019. <b>La Fig. 4<\/b> mostra l\u2019andamento del modulo di immagazzinamento di taglio (G\u2019, Pa) in funzione del tempo per il LunaGel\u2122 suino ad alta rigidit\u00e0 reticolato con 9,0 mW\/cm\u00b2 per 2, 4, 8 e 10 minuti, rispettivamente. Allo stesso modo, la luce \u00e8 stata programmata per accendersi dopo 2 minuti di prova con la durata indicata. Come previsto, le curve erano quasi identiche durante i primi 4 minuti. Dopo questo intervallo di tempo, il G\u2019 ha continuato ad aumentare in modo sostanziale nel tempo per i campioni sottoposti a un\u2019esposizione alla luce pi\u00f9 lunga.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a255815 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a255815\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"431\" height=\"401\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" class=\"attachment-large size-large wp-image-70803\" alt=\"G\u2019 over time for high stiffness porcine LunaGel\u2122 with variable light exposure\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?w=431&amp;ssl=1 431w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=300%2C279&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=157%2C146&amp;ssl=1 157w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=50%2C47&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?resize=81%2C75&amp;ssl=1 81w\" sizes=\"(max-width:767px) 431px, 431px\" data-attachment-id=\"70803\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/fig4_gelomics_ecm-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" data-orig-size=\"431,401\" data-comments-opened=\"1\" data-image-title=\"G\u2019 over time\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Fig4_Gelomics_ECM.jpg?fit=431%2C401&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0635d67 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"0635d67\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 4:  <\/strong>Modulo di conservazione al taglio (G\u2019, Pa) in funzione del tempo per il LunaGel\u2122 suino ad alta rigidit\u00e0 reticolato con luce a 405 nm (9,0 mW\/cm\u00b2) per 2, 4, 8 e 10 minuti.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9ca4a98 elementor-widget elementor-widget-text-editor\" data-id=\"9ca4a98\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Per studiare gli effetti della rigidit\u00e0 della MEC di LunaGel sul comportamento delle cellule tumorali al seno, gli sferoidi MDA-MB-231 sono stati seminati su MEC con modulo di conservazione al taglio (G\u2019) pari rispettivamente a circa 110, 220 e 440 kPa. Dopo 7 giorni di coltura, sono state osservate differenze significative nelle morfologie cellulari tra le diverse rigidit\u00e0 dell\u2019ECM (<b>Fig. 5<\/b>). L\u2019invasivit\u00e0 delle cellule tumorali al seno \u00e8 aumentata in funzione della rigidit\u00e0 dell\u2019ECM, confermando i dati clinici che suggeriscono una maggiore incidenza di metastasi nel cancro al seno con tessuti pi\u00f9 rigidi. Con l\u2019aumentare della rigidit\u00e0 dell\u2019ECM di LunaGel, si \u00e8 osservato uno spostamento verso morfologie cellulari pi\u00f9 migratorie, associate alle metastasi (Fig. 5, inserti ingranditi).   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-292155c elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"292155c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"355\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1024%2C355&amp;ssl=1\" class=\"attachment-large size-large wp-image-70804\" alt=\"Breast cancer cell behavior on LunaGel ECMs of varying stiffness\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?w=1415&amp;ssl=1 1415w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=300%2C104&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=1024%2C355&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=768%2C266&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=1200%2C416&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=260%2C90&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=50%2C17&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?resize=150%2C52&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"70804\" data-permalink=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/attachment\/figure-5_web-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1415%2C491&amp;ssl=1\" data-orig-size=\"1415,491\" data-comments-opened=\"1\" data-image-title=\"Breast cancer cell\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/2021\/11\/Figure-5_web.jpg?fit=1024%2C355&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-201a0a6 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"201a0a6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Fig. 5:  <\/strong>L&#8217;invasivit\u00e0 delle cellule tumorali al seno aumenta con l&#8217;aumentare della rigidit\u00e0 della matrice extracellulare (ECM). Le immagini mostrano sferoidi MDA-MB-231 seminati su ECM di gelatina bovina LunaGel con modulo di stoccaggio di taglio pari rispettivamente a (A) 110 Pa, (B) 220 Pa e (C) 440 Pa, dopo 7 giorni di coltura. Le cellule\/gli sferoidi sono stati colorati per evidenziare i nuclei cellulari (blu), l\u2019integrina \u03b21 (verde), la membrana cellulare (arancione) e l\u2019actina (magenta).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-298788a elementor-widget elementor-widget-heading\" data-id=\"298788a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Conclusioni<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a5f8f67 elementor-widget elementor-widget-text-editor\" data-id=\"a5f8f67\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>L&#8217;evoluzione viscoelastica dei prodotti ECM LunaGel\u2122 di Gelomics durante la reticolazione fotochimica \u00e8 stata monitorata con successo in tempo reale utilizzando <a href=\"https:\/\/rheolution.com\/it\/?p=70797\">ElastoSens\u2122<\/a><a href=\"https:\/\/rheolution.com\/it\/?p=70797\"> Bio.<\/a> Il valore finale di G\u2019 era circa 8 volte superiore nelle formulazioni ad alta rigidit\u00e0 rispetto a quelle a bassa rigidit\u00e0. Le propriet\u00e0 viscoelastiche finali di questi idrogel sono state facilmente regolate variando il tempo di esposizione alla luce durante la reticolazione (405 nm). L\u2019invasivit\u00e0 delle cellule tumorali al seno \u00e8 aumentata significativamente con l\u2019aumentare della rigidit\u00e0 dell\u2019ECM, dimostrando la capacit\u00e0 del sistema LunaGel\u2122 di riprodurre fedelmente le condizioni fisiologiche e fisiopatologiche dei tessuti.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afd817a elementor-widget elementor-widget-heading\" data-id=\"afd817a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Sintesi<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d936ff2 elementor-widget elementor-widget-text-editor\" data-id=\"d936ff2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul class=\"bullets\"><li class=\"bullets\">I prodotti LunaGel\u2122 permettono di ottenere idrogel con la rigidit\u00e0 desiderata.<\/li><li class=\"bullets\"><a href=\"https:\/\/rheolution.com\/it\/?p=70797\">ElastoSens\u2122 Bio<\/a> \u00e8 in grado di applicare luce (a diverse lunghezze d&#8217;onda) durante la misurazione della viscoelasticit\u00e0 degli idrogel, fornendo in tempo reale i dati relativi alla cinetica della loro fotoreticolazione.<\/li><li class=\"bullets\">Con <a href=\"https:\/\/rheolution.com\/it\/?p=70797\">ElastoSens\u2122 Bio<\/a> \u00e8 possibile combinare facilmente le intensit\u00e0 luminose e i tempi di esposizione durante il fotocrosslinking degli idrogel, per ottenere la viscoelasticit\u00e0 desiderata per l\u2019applicazione specifica.<\/li><li class=\"bullets\">Gli ECM LunaGel\u2122 permettono di studiare il comportamento delle cellule in funzione della rigidit\u00e0 dell&#8217;ECM.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-650fa95 elementor-widget elementor-widget-heading\" data-id=\"650fa95\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Riferimenti<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec1a35b elementor-widget elementor-widget-text-editor\" data-id=\"ec1a35b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>[1] Keely, P., &#038; Nain, A. (2015). <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4754037\/\" target=\"_blank\" rel=\"noopener\">Capturing relevant extracellular matrices for investigating cell migration<\/a>. F1000Research, 4.<\/p><p>[2] Wei, S. C., Fattet, L., Tsai, J. H., Guo, Y., Pai, V. H., Majeski, H. E., &#8230; &#038; Yang, J. (2015). <a href=\"https:\/\/www.nature.com\/articles\/ncb3157\" target=\"_blank\" rel=\"noopener\">Matrix stiffness drives epithelial\u2013mesenchymal transition and tumour metastasis through a TWIST1\u2013G3BP2 mechanotransduction pathway<\/a>. Nature cell biology, 17(5), 678-688.<\/p><p>[3] Paszek, M. J., Zahir, N., Johnson, K. R., Lakins, J. N., Rozenberg, G. I., Gefen, A., &#8230; &#038; Weaver, V. M. (2005). <a href=\"https:\/\/www.cell.com\/cancer-cell\/fulltext\/S1535-6108(05)00268-0\" target=\"_blank\" rel=\"noopener\">Tensional homeostasis and the malignant phenotype<\/a>. Cancer cell, 8(3), 241-254.<\/p><p>[4] Caliari, S. R., &#038; Burdick, J. A. (2016). <a href=\"https:\/\/www.nature.com\/articles\/nmeth.3839.pdf\" target=\"_blank\" rel=\"noopener\">A practical guide to hydrogels for cell culture<\/a>. Nature methods, 13(5), 405-414.<br\/><\/p><p> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-93251b6 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"93251b6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/wp-content\/uploads\/Viscoelasticity-of-Gelomics-LunaGel-Photocrosslinkable-ECM.pdf\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Accedi alla versione completa della nota sull'app (PDF) <\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-55b7ef2 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"55b7ef2\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-c97f30c swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"elastosens2\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a31667a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"a31667a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Scopri come la nostra tecnologia misura in modo non distruttivo le propriet\u00e0 viscoelastiche dei biomateriali morbidi e dei tessuti utilizzando microvolumi di campioni<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca42a8b elementor-align-center elementor-widget elementor-widget-button\" data-id=\"ca42a8b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/it\/contatti\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Contattaci per saperne di pi\u00f9<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8418b6a elementor-widget elementor-widget-heading\" data-id=\"8418b6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Articoli correlati<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6c98ea9 premium-blog-cta-full-yes premium-floating-effects-yes premium-disable-fe-yes elementor-widget__width-initial premium-blog-align-left elementor-widget elementor-widget-premium-addon-blog\" data-id=\"6c98ea9\" data-element_type=\"widget\" data-e-type=\"widget\" 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class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Regolazione della rigidit\u00e0 degli idrogel a base di ECM<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/\" target=\"_blank\">\n\t\t\t\tRegolazione della rigidit\u00e0 degli idrogel a base di ECM\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Tutte le cellule del corpo umano sono esposte a forze meccaniche che regolano la loro funzione e lo sviluppo dei tessuti, e ogni tipo di cellula si \u00e8 adattato in modo specifico alle propriet\u00e0 meccaniche del tessuto in cui si trova. Le propriet\u00e0 della matrice dei tessuti umani possono anche cambiare in presenza di una malattia e, a loro volta, favorirne la progressione.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/it\/note-applicative\/viscoelasticita-della-gelomica-lunagel-ecm-fotoreticolabile\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leggi di pi\u00f9 \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Tutte le cellule del corpo umano sono esposte a forze meccaniche che regolano la loro funzione e lo sviluppo dei tessuti, e ogni tipo di cellula si \u00e8 adattato in modo specifico alle propriet\u00e0 meccaniche del tessuto in cui si trova. Le propriet\u00e0 della matrice dei tessuti umani possono anche cambiare in presenza di una malattia e, a loro volta, favorirne la progressione.   <\/p>\n","protected":false},"author":195401338,"featured_media":70806,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[289,374,375,297],"tags":[89,92,94,91,124,88,146,99,105,87],"class_list":["post-70797","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-note-applicative","category-applicazioni-nellingegneria-tissutale","category-fotoreticolazione","category-application-formulation-of-hydrogels","tag-elastosens-bio","tag-hydrogel-formation","tag-hydrogel-with-cells","tag-hydrogels","tag-life-sciences","tag-mechanical-testing","tag-natural-polymer","tag-photocrosslinking","tag-tissue-engineering","tag-viscoelasticity"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Regolazione della Rigidit\u00e0 degli Idrogel ECM per la Coltura Cellulare<\/title>\n<meta name=\"description\" content=\"Misura in tempo reale la viscoelasticit\u00e0 e la fotoreticolazione degli idrogel di ECM con ElastoSens\u2122 Bio. 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