{"id":70904,"date":"2026-02-03T16:34:19","date_gmt":"2026-02-03T21:34:19","guid":{"rendered":"https:\/\/rheolution.com\/?p=70904"},"modified":"2026-10-08T15:59:10","modified_gmt":"2026-10-08T19:59:10","slug":"reticulation-de-la-gelatine-par-la-transglutaminase-microbienne","status":"publish","type":"post","link":"https:\/\/rheolution.com\/fr\/notes-application\/reticulation-de-la-gelatine-par-la-transglutaminase-microbienne\/","title":{"rendered":"R\u00e9ticulation de la g\u00e9latine \u00e0 l&rsquo;aide de la transglutaminase microbienne"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70904\" class=\"elementor elementor-70904 elementor-63479\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8fd4dcf e-flex e-con-boxed e-con e-parent\" data-id=\"8fd4dcf\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8c770e2 elementor-widget elementor-widget-heading\" data-id=\"8c770e2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Note d\u2019application | ElastoSens\u2122 Bio<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-760ae40 elementor-widget elementor-widget-heading\" data-id=\"760ae40\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">R\u00e9ticulation de la g\u00e9latine \u00e0 l'aide de la transglutaminase microbienne : caract\u00e9risation visco\u00e9lastique\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-16057f6 elementor-widget elementor-widget-text-editor\" data-id=\"16057f6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>par <a href=\"https:\/\/www.linkedin.com\/in\/mayasalame\/\">Maya Salame<\/a> et <a href=\"https:\/\/www.linkedin.com\/in\/dimitria-camas%C3%A3o-049002a2\/\">Dimitria Camasao<\/a><\/p><p>Charg\u00e9s d&rsquo;\u00e9tudes d&rsquo;application, <a href=\"https:\/\/www.linkedin.com\/company\/1168758\" target=\"_blank\" rel=\"noopener\">Rheolution Inc.<\/a><\/p><p>En collaboration avec  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-414c0c0 e-grid e-con-full e-con e-child\" data-id=\"414c0c0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4585270 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"4585270\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"350\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1024%2C350&amp;ssl=1\" class=\"attachment-large size-large wp-image-63679\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?w=1805&amp;ssl=1 1805w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=300%2C103&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=1024%2C350&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=768%2C263&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=1536%2C525&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=150%2C51&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?resize=700%2C239&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63679\" data-permalink=\"https:\/\/rheolution.com\/fr\/zedira-logo-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1805%2C617&amp;ssl=1\" data-orig-size=\"1805,617\" data-comments-opened=\"1\" data-image-meta=\"{&quot;credit&quot;:&quot;bott&quot;,&quot;created_timestamp&quot;:&quot;1466443687&quot;}\" data-image-title=\"Zedira Logo\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Zedira-Logo.jpg?fit=1024%2C350&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-252ef73 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"252ef73\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"318\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=1024%2C318&amp;ssl=1\" class=\"attachment-large size-large wp-image-63671\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=4126&amp;ssl=1 4126w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=300%2C93&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=1024%2C318&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=768%2C238&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=1536%2C477&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=2048%2C635&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=150%2C47&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?resize=700%2C217&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63671\" data-permalink=\"https:\/\/rheolution.com\/fr\/logo_rousselot_biomedical_tagline-1_rgb-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=4126%2C1280&amp;ssl=1\" data-orig-size=\"4126,1280\" data-comments-opened=\"1\" data-image-title=\"Logo_Rousselot_Biomedical_Tagline-1_RGB\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Logo_Rousselot_Biomedical_Tagline-1_RGB.png?fit=1024%2C318&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-97ac8de e-flex e-con-boxed e-con e-parent\" data-id=\"97ac8de\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5fc8fde elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5fc8fde\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">R\u00e9sum\u00e9<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d61237b elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"d61237b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Protocole de r\u00e9f\u00e9rence \u00e9tabli pour la r\u00e9ticulation enzymatique de la g\u00e9latine X-Pure Gelatin\u00ae \u00e0 l&rsquo;aide de la transglutaminase microbienne Andracon\u00ae, avec surveillance m\u00e9canique en temps r\u00e9el \u00e0 l&rsquo;aide d&rsquo;ElastoSens\u2122 Bio.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">L&rsquo;augmentation de la concentration en g\u00e9latine a entra\u00een\u00e9 une hausse significative de la rigidit\u00e9 finale du gel (G\u2032) et a r\u00e9duit le temps de g\u00e9lification.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">L&rsquo;augmentation de la concentration en MTG a permis d&rsquo;accro\u00eetre la r\u00e9sistance m\u00e9canique finale et d&rsquo;acc\u00e9l\u00e9rer consid\u00e9rablement la g\u00e9lification et la stabilisation du r\u00e9seau.<\/span><span style=\"font-weight: 400;\"><br><\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">La temp\u00e9rature de r\u00e9ticulation (37 \u00b0C contre 45 \u00b0C) n&rsquo;a pas eu d&rsquo;effet significatif sur les propri\u00e9t\u00e9s visco\u00e9lastiques finales, ce qui confirme la compatibilit\u00e9 avec les conditions physiologiques.<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Il a \u00e9t\u00e9 d\u00e9montr\u00e9 qu\u2019ElastoSens\u2122 Bio constituait un outil pr\u00e9cieux pour \u00e9tablir des protocoles de r\u00e9f\u00e9rence relatifs \u00e0 la r\u00e9ticulation enzymatique de la g\u00e9latine et pour \u00e9tudier l\u2019influence des param\u00e8tres de formulation et de proc\u00e9d\u00e9 sur la cin\u00e9tique de g\u00e9lification et les propri\u00e9t\u00e9s m\u00e9caniques.<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-82969b3 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"82969b3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Introduction<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5703c6a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5703c6a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">La g\u00e9latine : un biopolym\u00e8re polyvalent<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38524ee elementor-widget elementor-widget-text-editor\" data-id=\"38524ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">La g\u00e9latine est un biopolym\u00e8re largement utilis\u00e9 dans le domaine des biomat\u00e9riaux, car elle est transformable en milieu aqueux, biocompatible et capable de former des r\u00e9seaux souples et hydrat\u00e9s. Cependant, les gels de g\u00e9latine physiques peuvent s&rsquo;affaiblir ou fondre \u00e0 des temp\u00e9ratures proches de celles du corps humain ; c&rsquo;est pourquoi on recourt couramment \u00e0 la r\u00e9ticulation covalente pour am\u00e9liorer leur stabilit\u00e9 thermique et leur int\u00e9grit\u00e9 m\u00e9canique.   <\/span><a href=\"https:\/\/www.darlingii.com\/rousselot\/brands\/xpure\"><span style=\"font-weight: 400;\">X-Pure Gelatin\u00ae<\/span><\/a><span style=\"font-weight: 400;\"> est une g\u00e9latine de haute qualit\u00e9, de qualit\u00e9 pharmaceutique, qui se caract\u00e9rise par des normes de puret\u00e9 rigoureuses et des performances constantes.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-29574d5 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"29574d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">R\u00e9ticulation des polym\u00e8res \u00e0 l'aide de la transglutaminase microbienne (MTG) Andracon\u00ae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2e827a1 elementor-widget elementor-widget-text-editor\" data-id=\"2e827a1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">L&rsquo;agent de r\u00e9ticulation biologique, la transglutaminase microbienne, catalyse la formation de liaisons covalentes entre les r\u00e9sidus de glutamine et de lysine pr\u00e9sents dans la g\u00e9latine, permettant ainsi la formation contr\u00f4l\u00e9e d&rsquo;un r\u00e9seau dans des conditions aqueuses douces. <\/span><a href=\"https:\/\/zedira.com\/Microbial-Transglutaminase\"><span style=\"font-weight: 400;\">Andracon\u00ae<\/span><\/a><span style=\"font-weight: 400;\"> est une transglutaminase microbienne de haute qualit\u00e9 destin\u00e9e \u00e0 la recherche pr\u00e9clinique ainsi qu\u2019aux applications cliniques.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-885800d elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"885800d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u00c9tude de cas : Caract\u00e9risation m\u00e9canique de la r\u00e9ticulation de la g\u00e9latine par la transglutaminase \u00e0 l'aide d'ElastoSens\u2122 Bio<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b87c673 elementor-widget elementor-widget-text-editor\" data-id=\"b87c673\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Dans le cadre de ces travaux, un protocole de r\u00e9ticulation de r\u00e9f\u00e9rence a \u00e9t\u00e9 d\u00e9fini \u00e0 l\u2019aide de g\u00e9latine X-Pure Gelatin\u00ae d\u2019origine porcine acide et d\u2019Andracon\u00ae MTG, et la g\u00e9lification a \u00e9t\u00e9 suivie de mani\u00e8re non destructive \u00e0 l\u2019aide du syst\u00e8me ElastoSens\u2122 Bio. Cet instrument a permis de quantifier l\u2019\u00e9volution visco\u00e9lastique en fonction du temps des formulations de g\u00e9latine-MTG au cours de la r\u00e9ticulation. Le module de stockage en cisaillement (G\u2032) d\u00e9crit la composante \u00e9lastique du mat\u00e9riau, qui emmagasine de l\u2019\u00e9nergie, et est g\u00e9n\u00e9ralement interpr\u00e9t\u00e9 comme un indicateur de la rigidit\u00e9 du gel au fur et \u00e0 mesure que le r\u00e9seau se forme. Le module de perte en cisaillement (G\u2033) repr\u00e9sente la composante visqueuse, qui dissipe l\u2019\u00e9nergie, et refl\u00e8te la contribution de type fluide pendant la r\u00e9ticulation. Le facteur d\u2019amortissement (tan \u03b4 = G\u2033\/G\u2032) fournit une mesure adimensionnelle de l\u2019\u00e9quilibre entre les r\u00e9ponses visqueuses et \u00e9lastiques et varie de 0 (comportement de type liquide, G\u2033 dominant) \u00e0 1 (comportement de type solide, G\u2032 dominant). Le point de g\u00e9lification (transition sol-gel) a \u00e9t\u00e9 d\u00e9fini comme le moment o\u00f9 G\u2032 = G\u2033 ; le temps de g\u00e9lification a \u00e9t\u00e9 exprim\u00e9 comme le temps \u00e9coul\u00e9 entre le d\u00e9but de la mesure et ce croisement. La surveillance continue a permis d\u2019obtenir des courbes cin\u00e9tiques de g\u00e9lification compl\u00e8tes, ce qui a permis (i) d\u2019identifier le moment o\u00f9 le signal atteignait une zone de plateau et (ii) de calculer les valeurs finales stabilis\u00e9es de G\u2032, G\u2033 et tan \u03b4 en effectuant une moyenne sur ce plateau. Ces m\u00eames courbes cin\u00e9tiques ont \u00e9galement \u00e9t\u00e9 utilis\u00e9es pour d\u00e9terminer le temps de plateau, d\u00e9fini comme le temps total n\u00e9cessaire \u00e0 la stabilisation des propri\u00e9t\u00e9s visco\u00e9lastiques (G\u2032 atteignant 90 % de sa valeur finale (de plateau)).         <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df34a6d elementor-widget-mobile__width-initial elementor-widget elementor-widget-image\" data-id=\"df34a6d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"968\" height=\"544\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" class=\"attachment-large size-large wp-image-50363\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?w=968&amp;ssl=1 968w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=300%2C169&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=768%2C432&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=133%2C75&amp;ssl=1 133w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?resize=480%2C270&amp;ssl=1 480w\" sizes=\"(max-width:767px) 700px, (max-width:968px) 100vw, 968px\" data-attachment-id=\"50363\" data-permalink=\"https:\/\/rheolution.com\/fr\/elastosens2-4-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" data-orig-size=\"968,544\" data-comments-opened=\"1\" data-image-title=\"elastosens2\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/elastosens2-1.webp?fit=968%2C544&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ed23fc9 elementor-widget elementor-widget-heading\" data-id=\"ed23fc9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Mat\u00e9riel et m\u00e9thodes<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d118051 elementor-widget elementor-widget-text-editor\" data-id=\"d118051\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">G\u00e9latine acide issue de peau de porc (<\/span><a href=\"https:\/\/www.darlingii.com\/en\/rousselot\/brands\/xpure\"><span style=\"font-weight: 400;\">X-Pure Gelatin\u00ae, Rousselot<\/span><\/a><span style=\"font-weight: 400;\">; HBHV, environ 300 Bloom) a \u00e9t\u00e9 r\u00e9ticul\u00e9e \u00e0 l&rsquo;aide de transglutaminase microbienne (<\/span><a href=\"https:\/\/zedira.com\/Microbial-Transglutaminase\"><span style=\"font-weight: 400;\">Andracon\u00ae, Zedira<\/span><\/a><span style=\"font-weight: 400;\">) et son comportement a \u00e9t\u00e9 suivi en temps r\u00e9el \u00e0 l\u2019aide de l\u2019ElastoSens\u2122 Bio (<\/span><a href=\"https:\/\/rheolution.com\/fr\/produits\/elastosens-bio\/instrument\/\"><span style=\"font-weight: 400;\">Rheolution<\/span><\/a><span style=\"font-weight: 400;\">). <\/span> <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4499b74 elementor-widget elementor-widget-heading\" data-id=\"4499b74\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>Conditions de test :<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d014f83 elementor-widget elementor-widget-text-editor\" data-id=\"d014f83\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Concentration en g\u00e9latine : 5, 10, 15 % en poids  <\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Chargement MTG : 15 et 30 U g\u207b\u00b9 de g\u00e9latine<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Temp\u00e9rature : 37 \u00b0C et 45 \u00b0C<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Volume d&rsquo;\u00e9chantillon ElastoSens : 2,0 ml<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Dur\u00e9e de la mesure : 6 h<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">R\u00e9plicats : n = 3 par condition<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23a931e elementor-widget elementor-widget-text-editor\" data-id=\"23a931e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Des solutions-m\u00e8res de g\u00e9latine ont \u00e9t\u00e9 pr\u00e9par\u00e9es \u00e0 5 % en poids (50 mg\/mL), 10 % en poids (100 mg\/mL) et 15 % en poids (150 mg\/mL) en dissolvant la g\u00e9latine dans du PBS. Les solutions ont \u00e9t\u00e9 incub\u00e9es dans un bain-marie \u00e0 60 \u00b0C pendant 60 minutes sous agitation magn\u00e9tique continue afin d\u2019assurer une dissolution compl\u00e8te. Parall\u00e8lement, la solution m\u00e8re de l\u2019enzyme Andracon\u00ae (activit\u00e9 totale de 116 U) a \u00e9t\u00e9 pr\u00e9par\u00e9e dans de l\u2019eau d\u00e9ionis\u00e9e sous forme de solution de travail \u00e0 341,2 U\/mL, puis mise \u00e0 \u00e9quilibre \u00e0 37 \u00b0C pendant 5 minutes avant utilisation (conserv\u00e9e \u00e0 4 \u00b0C le reste du temps).  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58a09c6 elementor-widget elementor-widget-heading\" data-id=\"58a09c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>Chargement des \u00e9chantillons et mesure ElastoSens\u2122 Bio :<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b9dfced elementor-widget elementor-widget-text-editor\" data-id=\"b9dfced\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">La r\u00e9ticulation a \u00e9t\u00e9 d\u00e9clench\u00e9e par l&rsquo;ajout du volume calcul\u00e9 de solution m\u00e8re de MTG \u00e0 la solution de g\u00e9latine afin d&rsquo;atteindre la valeur cible en U g\u207b\u00b9 (tableau 1).<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Les \u00e9chantillons ont \u00e9t\u00e9 m\u00e9lang\u00e9s d\u00e9licatement \u00e0 l&rsquo;aide d&rsquo;une barre magn\u00e9tique pendant quelques secondes afin d&rsquo;obtenir un m\u00e9lange homog\u00e8ne.<\/span><\/li><li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">L&rsquo;appareil a \u00e9t\u00e9 pr\u00e9alablement stabilis\u00e9 \u00e0 la temp\u00e9rature cible (37 \u00b0C ou 45 \u00b0C). Imm\u00e9diatement apr\u00e8s le m\u00e9lange, 2,0 ml du m\u00e9lange r\u00e9actionnel ont \u00e9t\u00e9 transf\u00e9r\u00e9s dans le porte-\u00e9chantillon macro et la mesure s&rsquo;est d\u00e9roul\u00e9e en continu pendant 6 heures. <\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-294dbc1 elementor-widget elementor-widget-text-editor\" data-id=\"294dbc1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Tableau 1 :<\/b><span style=\"font-weight: 400;\"> Volume de transglutaminase microbienne (MTG) n\u00e9cessaire \u00e0 la r\u00e9ticulation de la g\u00e9latine, calcul\u00e9 pour un volume de pr\u00e9paration de 2,5 ml \u00e0 partir d&rsquo;une concentration de solution m\u00e8re de MTG de 341,2 U\/ml.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5929e04 eael-dt-th-align-center eael-table-align-center elementor-widget elementor-widget-eael-data-table\" data-id=\"5929e04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-data-table.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"eael-data-table-wrap\" data-table_id=\"5929e04\" id=\"eael-data-table-wrapper-5929e04\" data-custom_responsive=\"false\">\n\t\t\t<table class=\"tablesorter eael-data-table center\" id=\"eael-data-table-5929e04\">\n\t\t\t    <thead>\n\t\t\t        <tr class=\"table-header\">\n\t\t\t\t\t\t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Concentration en g\u00e9latine [% en poids]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Concentration en g\u00e9latine [g L\u207b\u00b9]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Quantit\u00e9 totale de g\u00e9latine dans 2,5 ml [g]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Dose cible de MTG [U g\u207b\u00b9]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Activit\u00e9 totale de l'enzyme MTG dans 2,5 ml [U]<\/span><\/th>\n\t\t\t        \t\t\t\t            <th class=\"\" id=\"\" colspan=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"data-table-header-text\">Volume de solution MTG [\u00b5L]<\/span><\/th>\n\t\t\t        \t\t\t\t        <\/tr>\n\t\t\t    <\/thead>\n\t\t\t  \t<tbody>\n\t\t\t\t\t\t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t50\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.125\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t3.75\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t10\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.250\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t7.50\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t22\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t15\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t150\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.375\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11.25\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t33\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t\t\t\t<tr>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t10\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t100\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t0.250\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t15\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t3.75\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t   \t\t\t\t\t\t\t\t\t\t\t<td colspan=\"\" rowspan=\"\" class=\"\" id=\"\">\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"td-content-wrapper\"><div class=\"td-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t11\t\t\t\t\t\t\t\t\t\t\t\t<\/div><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<\/td>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/tr>\n\t\t\t        \t\t\t    <\/tbody>\n\t\t\t<\/table>\n\t\t<\/div>\n\t  \t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d907f30 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"d907f30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"889\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1024%2C889&amp;ssl=1\" class=\"attachment-large size-large wp-image-63548\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?w=1240&amp;ssl=1 1240w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=300%2C260&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=1024%2C889&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=768%2C666&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=86%2C75&amp;ssl=1 86w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?resize=700%2C607&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63548\" data-permalink=\"https:\/\/rheolution.com\/fr\/sample-holder-with-sample-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1240%2C1076&amp;ssl=1\" data-orig-size=\"1240,1076\" data-comments-opened=\"1\" data-image-title=\"Sample Holder with Sample\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Sample-Holder-with-Sample.jpg?fit=1024%2C889&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-39e927a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"39e927a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 1.<\/b><span style=\"font-weight: 400;\">  Hydrogels de g\u00e9latine repr\u00e9sentatifs (n = 3) apr\u00e8s leur retrait du porte-\u00e9chantillons macro \u00e0 l&rsquo;issue de l&rsquo;exp\u00e9rience de g\u00e9lification.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8cc0f04 elementor-widget elementor-widget-heading\" data-id=\"8cc0f04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\"><u>Analyse statistique :<\/u><\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-54513af elementor-widget elementor-widget-text-editor\" data-id=\"54513af\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Les donn\u00e9es ont \u00e9t\u00e9 analys\u00e9es par une ANOVA \u00e0 un facteur (test de Brown-Forsythe\/Welch pour tenir compte des variances in\u00e9gales). Les comparaisons par paires pr\u00e9vues ont \u00e9t\u00e9 r\u00e9alis\u00e9es \u00e0 l&rsquo;aide de tests t de Welch non appari\u00e9s (sans ajustement pour les comparaisons multiples). Les donn\u00e9es sont exprim\u00e9es sous forme de moyenne \u00b1 \u00e9cart-type ;    <\/span><i><span style=\"font-weight: 400;\">p<\/span><\/i><span style=\"font-weight: 400;\">&lt; 0,05.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3ded5ea elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"3ded5ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Comportement visco\u00e9lastique lors de la r\u00e9ticulation de la g\u00e9latine : r\u00e9sultats et discussion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca90a6e elementor-widget elementor-widget-text-editor\" data-id=\"ca90a6e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Cette \u00e9tude a \u00e9t\u00e9 con\u00e7ue pour \u00e9valuer la r\u00e9ticulation enzymatique de la g\u00e9latine par la MTG et pour \u00e9tudier l\u2019influence de param\u00e8tres cl\u00e9s sur le comportement de g\u00e9lification et les propri\u00e9t\u00e9s m\u00e9caniques. Plus pr\u00e9cis\u00e9ment, les effets de la concentration en g\u00e9latine, de la concentration en transglutaminase et de la temp\u00e9rature de r\u00e9ticulation ont \u00e9t\u00e9 examin\u00e9s. Dans la majorit\u00e9 des exp\u00e9riences, une temp\u00e9rature de r\u00e9ticulation de 45 \u00b0C a \u00e9t\u00e9 utilis\u00e9e afin d\u2019\u00e9viter la g\u00e9lification thermique de la g\u00e9latine, garantissant ainsi que la g\u00e9lification \u00e9tait exclusivement due \u00e0 la r\u00e9ticulation enzymatique. Cette temp\u00e9rature se situe \u00e9galement dans une plage favorable \u00e0 une activit\u00e9 enzymatique \u00e9lev\u00e9e et stable.   <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2f0036 elementor-widget elementor-widget-heading\" data-id=\"b2f0036\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Influence de la concentration en g\u00e9latine sur la g\u00e9lification et les propri\u00e9t\u00e9s m\u00e9caniques<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-213f704 elementor-widget elementor-widget-text-editor\" data-id=\"213f704\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">La figure 2 illustre l&rsquo;\u00e9volution du module de stockage en cisaillement (G\u2032) en fonction du temps au cours de la r\u00e9ticulation de la g\u00e9latine par la transglutaminase microbienne. La ligne en gras repr\u00e9sente la moyenne des trois r\u00e9p\u00e9titions, tandis que les lignes pointill\u00e9es repr\u00e9sentent l&rsquo;\u00e9cart-type. Pour tous les \u00e9chantillons, G\u2032 a commenc\u00e9 \u00e0 de faibles valeurs et a connu une forte augmentation apr\u00e8s le point de transition liquide-gel, pour finalement atteindre un plateau.    <\/span><\/p><p><span style=\"font-weight: 400;\">La figure 3 pr\u00e9sente les valeurs moyennes de G\u2032 et G\u2033 au cours de la derni\u00e8re heure de l&rsquo;essai. Comme pr\u00e9vu, la valeur finale de G\u2032 a augment\u00e9 avec l\u2019augmentation de la concentration en g\u00e9latine (***p &lt; 0,001), ce qui indique que le r\u00e9seau form\u00e9 est plus r\u00e9sistant en raison de la pr\u00e9sence d\u2019un plus grand nombre de mol\u00e9cules de g\u00e9latine. G\u2019\u2019 a \u00e9galement affich\u00e9 une tendance similaire ; toutefois, aucune diff\u00e9rence statistique n\u2019a \u00e9t\u00e9 constat\u00e9e dans cette \u00e9tude. Le facteur d\u2019amortissement (tan \u03b4 = G\u2033\/G\u2032) \u00e9tait similaire entre les conditions \u00e0 15 % en masse et \u00e0 10 % en masse, et l\u00e9g\u00e8rement plus \u00e9lev\u00e9 pour la condition \u00e0 5 % en masse, ce qui indique une pr\u00e9dominance visqueuse plus importante dans cette derni\u00e8re par rapport aux autres.   <\/span><\/p><p><span style=\"font-weight: 400;\">La figure 4 pr\u00e9sente le temps n\u00e9cessaire pour atteindre le point de transition liquide-gel (G\u2019 = G\u2019\u2019, \u00e0 gauche) et le temps total n\u00e9cessaire pour atteindre le plateau (\u00e0 droite). Comme pr\u00e9vu, le temps n\u00e9cessaire pour atteindre la transition liquide-gel a diminu\u00e9 avec l\u2019augmentation de la concentration en g\u00e9latine et s\u2019est \u00e9lev\u00e9 \u00e0 environ 0,5, 10 et 14 minutes pour les \u00e9chantillons \u00e0 15 %, 10 % et 5 % en poids, respectivement. Ce comportement s\u2019explique par la plus grande disponibilit\u00e9 des cha\u00eenes polym\u00e8res de g\u00e9latine \u00e0 des concentrations plus \u00e9lev\u00e9es, ce qui favorise une formation plus rapide du r\u00e9seau et acc\u00e9l\u00e8re la transition liquide-gel. Il est int\u00e9ressant de noter que, bien que chaque condition ait n\u00e9cessit\u00e9 un temps diff\u00e9rent pour atteindre le point de transition, toutes ont mis un temps similaire pour atteindre le plateau, ce qui sugg\u00e8re qu\u2019une fois la g\u00e9lification amorc\u00e9e, la cin\u00e9tique de r\u00e9ticulation qui s\u2019ensuit est principalement r\u00e9gie par l\u2019activit\u00e9 enzymatique plut\u00f4t que par la concentration en g\u00e9latine.   <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a19c2ed elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a19c2ed\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"640\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1024%2C640&amp;ssl=1\" class=\"attachment-large size-large wp-image-63540\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?w=1542&amp;ssl=1 1542w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=300%2C187&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=1024%2C640&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=768%2C480&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=1536%2C959&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=120%2C75&amp;ssl=1 120w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=960%2C600&amp;ssl=1 960w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?resize=700%2C437&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63540\" data-permalink=\"https:\/\/rheolution.com\/fr\/15-10-5-compare-gelatin-g-kinetics-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1542%2C963&amp;ssl=1\" data-orig-size=\"1542,963\" data-comments-opened=\"1\" data-image-title=\"15%, 10%, 5% compare [gelatin] &amp;#8211; G&amp;rsquo; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-5-compare-gelatin-G-kinetics.png?fit=1024%2C640&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6327b34 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"6327b34\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 2.<\/b><span style=\"font-weight: 400;\">  \u00c9volution du module de stockage en cisaillement (G\u2032) au cours de la g\u00e9lification d&rsquo;hydrogels de g\u00e9latine \u00e0 15 %, 10 % et 5 % en masse, r\u00e9ticul\u00e9s \u00e0 45 \u00b0C avec 30 U g\u207b\u00b9 de transglutaminase.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a08afe4 e-grid e-con-full e-con e-child\" data-id=\"a08afe4\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0cfd1bb elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"0cfd1bb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"740\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=740%2C1024&amp;ssl=1\" class=\"attachment-large size-large wp-image-63500\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?w=750&amp;ssl=1 750w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=217%2C300&amp;ssl=1 217w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=740%2C1024&amp;ssl=1 740w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=54%2C75&amp;ssl=1 54w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?resize=700%2C969&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 740px\" data-attachment-id=\"63500\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-effect-g-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=750%2C1038&amp;ssl=1\" data-orig-size=\"750,1038\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; G&amp;rsquo; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph.png?fit=740%2C1024&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-683a272 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"683a272\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"667\" height=\"1013\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" class=\"attachment-large size-large wp-image-63524\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?w=667&amp;ssl=1 667w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?resize=198%2C300&amp;ssl=1 198w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?resize=49%2C75&amp;ssl=1 49w\" sizes=\"(max-width:767px) 667px, 667px\" data-attachment-id=\"63524\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-effect-g-bar-graph-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" data-orig-size=\"667,1013\" data-comments-opened=\"1\" data-image-title=\"gelatin effect- G\u00a0\u00bb Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-G-Bar-graph-1.png?fit=667%2C1013&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1950aba elementor-widget elementor-widget-image\" data-id=\"1950aba\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"688\" height=\"942\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" class=\"attachment-large size-large wp-image-63532\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?w=688&amp;ssl=1 688w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?resize=219%2C300&amp;ssl=1 219w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?resize=55%2C75&amp;ssl=1 55w\" sizes=\"(max-width:767px) 688px, 688px\" data-attachment-id=\"63532\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-effect-tand-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" data-orig-size=\"688,942\" data-comments-opened=\"1\" data-image-title=\"gelatin effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-TanD-Bar-graph.png?fit=688%2C942&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f383e58 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f383e58\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 3.<\/b><span style=\"font-weight: 400;\">  Propri\u00e9t\u00e9s visco\u00e9lastiques finales des hydrogels de g\u00e9latine r\u00e9ticul\u00e9s par la transglutaminase \u00e0 45 \u00b0C. Le module de stockage en cisaillement (G\u2032, \u00e0 gauche), le module de perte en cisaillement (G\u2033, au centre) et le facteur d\u2019amortissement (tan \u03b4, \u00e0 droite) ont \u00e9t\u00e9 calcul\u00e9s \u00e0 partir de la zone de plateau (5 \u00e0 6 h) des courbes de g\u00e9lification d\u2019hydrogels de g\u00e9latine \u00e0 15, 10 et 5 % en poids d\u2019hydrogels de g\u00e9latine r\u00e9ticul\u00e9s avec 30 U g\u207b\u00b9 de transglutaminase. Les barres indiquent la moyenne \u00b1 l\u2019\u00e9cart-type, les points de donn\u00e9es individuels \u00e9tant superpos\u00e9s. La signification statistique est indiqu\u00e9e par *p &lt; 0,05, **p &lt; 0,01, ****p &lt; 0,0001 ; ns, non significatif.   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-ee8f627 e-grid e-con-full e-con e-child\" data-id=\"ee8f627\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0cfcaef elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"0cfcaef\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"703\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=703%2C1024&amp;ssl=1\" class=\"attachment-large size-large wp-image-63508\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?w=713&amp;ssl=1 713w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=206%2C300&amp;ssl=1 206w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=703%2C1024&amp;ssl=1 703w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=52%2C75&amp;ssl=1 52w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?resize=700%2C1019&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 703px\" data-attachment-id=\"63508\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-effect-sol-gel-transition-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=713%2C1038&amp;ssl=1\" data-orig-size=\"713,1038\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-sol-gel-transition.png?fit=703%2C1024&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d66ae20 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"d66ae20\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"663\" height=\"1013\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" class=\"attachment-large size-large wp-image-63516\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?w=663&amp;ssl=1 663w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?resize=196%2C300&amp;ssl=1 196w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?resize=49%2C75&amp;ssl=1 49w\" sizes=\"(max-width:767px) 663px, 663px\" data-attachment-id=\"63516\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-effect-time-to-plateau-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" data-orig-size=\"663,1013\" data-comments-opened=\"1\" data-image-title=\"gelatin effect &amp;#8211; Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/gelatin-effect-Time-to-plateau.png?fit=663%2C1013&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-65db86c elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"65db86c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 4.<\/b><span style=\"font-weight: 400;\">  Le temps de g\u00e9lification, d\u00e9fini par le croisement G\u2032\u2013G\u2033 (\u00e0 gauche), et le temps n\u00e9cessaire pour atteindre le plateau visco\u00e9lastique (\u00e0 droite ; temps n\u00e9cessaire pour que G\u2032 atteigne 90 % de sa valeur finale stabilis\u00e9e) pour des concentrations de 5, 10 et 15 % en poids de g\u00e9latine r\u00e9ticul\u00e9e par la transglutaminase (30 U g\u207b\u00b9 \u00e0 45 \u00b0C). Les barres repr\u00e9sentent la moyenne \u00b1 l&rsquo;\u00e9cart-type, les r\u00e9plicats individuels \u00e9tant superpos\u00e9s. La signification statistique est indiqu\u00e9e comme suit : *p &lt; 0,05, **p&lt; 0,01, ***p &lt; 0,001 ; ns, non significatif.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-794c36a elementor-widget elementor-widget-heading\" data-id=\"794c36a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Influence de la concentration en transglutaminase sur la g\u00e9lification et les propri\u00e9t\u00e9s m\u00e9caniques<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aed2e2d elementor-widget elementor-widget-text-editor\" data-id=\"aed2e2d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">La figure 5 illustre l\u2019\u00e9volution dans le temps du module de stockage en cisaillement (G\u2032) au cours de la r\u00e9ticulation de la g\u00e9latine par la transglutaminase microbienne \u00e0 deux concentrations enzymatiques diff\u00e9rentes. La ligne continue correspond \u00e0 la moyenne de trois mesures, tandis que les lignes pointill\u00e9es indiquent l&rsquo;\u00e9cart-type. Comme pr\u00e9c\u00e9demment, G\u2032 est d&rsquo;abord rest\u00e9 faible, puis a augment\u00e9 de mani\u00e8re marqu\u00e9e apr\u00e8s la transition liquide-gel, pour finalement atteindre un plateau stable.  <\/span><\/p><p><span style=\"font-weight: 400;\">La figure 6 pr\u00e9sente les valeurs moyennes de G\u2032 et G\u2033 au cours de la derni\u00e8re heure de l&rsquo;essai. La valeur finale de G\u2032 a augment\u00e9 avec l&rsquo;augmentation de la concentration enzymatique (**p &lt; 0,01), car des concentrations enzymatiques plus \u00e9lev\u00e9es permettent la formation d&rsquo;un plus grand nombre de liaisons transversales au cours de la m\u00eame p\u00e9riode d&rsquo;\u00e9valuation. Aucune diff\u00e9rence statistique n&rsquo;a \u00e9t\u00e9 observ\u00e9e pour G\u2019\u2019 tan \u03b4.  <\/span><\/p><p><span style=\"font-weight: 400;\">La figure 7 pr\u00e9sente le temps n\u00e9cessaire pour atteindre le point de transition liquide-gel (\u00e0 gauche) et le temps total n\u00e9cessaire pour atteindre le plateau (\u00e0 droite). Comme pr\u00e9vu, la condition pr\u00e9sentant une concentration enzymatique plus \u00e9lev\u00e9e a n\u00e9cessit\u00e9 moins de temps pour atteindre le point de transition liquide-gel en raison d\u2019une activit\u00e9 de r\u00e9ticulation accrue. Dans ce cas, le temps n\u00e9cessaire pour atteindre le plateau \u00e9tait \u00e9galement nettement plus court \u00e0 des concentrations enzymatiques plus \u00e9lev\u00e9es, ce qui corrobore le constat pr\u00e9c\u00e9dent selon lequel ce param\u00e8tre d\u00e9pend davantage de la concentration enzymatique que de la concentration en g\u00e9latine.  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a99d963 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"a99d963\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"639\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1024%2C639&amp;ssl=1\" class=\"attachment-large size-large wp-image-63605\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?w=1563&amp;ssl=1 1563w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=300%2C187&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=1024%2C639&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=768%2C480&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=1536%2C959&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=120%2C75&amp;ssl=1 120w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=960%2C600&amp;ssl=1 960w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?resize=700%2C437&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63605\" data-permalink=\"https:\/\/rheolution.com\/fr\/15-10-compare-t300-g-kinetics-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1563%2C976&amp;ssl=1\" data-orig-size=\"1563,976\" data-comments-opened=\"1\" data-image-title=\"15%, 10% compare t300 &amp;#8211; G&amp;rsquo; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-10-compare-t300-G-kinetics.png?fit=1024%2C639&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f1ddc8d elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f1ddc8d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 5.<\/b><span style=\"font-weight: 400;\">  \u00c9volution du module de stockage en cisaillement (G\u2032) au cours de la g\u00e9lification d\u2019hydrogels de g\u00e9latine \u00e0 10 % en masse, r\u00e9ticul\u00e9s \u00e0 45 \u00b0C avec 30 U g\u207b\u00b9 et 15 U g\u207b\u00b9 de transglutaminase.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-8b274f3 e-grid e-con-full e-con e-child\" data-id=\"8b274f3\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fc14b22 elementor-widget elementor-widget-image\" data-id=\"fc14b22\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"750\" height=\"917\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" class=\"attachment-large size-large wp-image-63565\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?w=750&amp;ssl=1 750w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=245%2C300&amp;ssl=1 245w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=61%2C75&amp;ssl=1 61w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?resize=700%2C856&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 750px\" data-attachment-id=\"63565\" data-permalink=\"https:\/\/rheolution.com\/fr\/tg-effect-g-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" data-orig-size=\"750,917\" data-comments-opened=\"1\" data-image-title=\"tg effect &amp;#8211; G&amp;rsquo; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph.png?fit=750%2C917&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1c23c75 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"1c23c75\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"667\" height=\"905\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" class=\"attachment-large size-large wp-image-63573\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?w=667&amp;ssl=1 667w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?resize=221%2C300&amp;ssl=1 221w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?resize=55%2C75&amp;ssl=1 55w\" sizes=\"(max-width:767px) 667px, 667px\" data-attachment-id=\"63573\" data-permalink=\"https:\/\/rheolution.com\/fr\/tg-effect-g-bar-graph-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" data-orig-size=\"667,905\" data-comments-opened=\"1\" data-image-title=\"tg effect- G\u00a0\u00bb Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-G-Bar-graph-1.png?fit=667%2C905&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6ec4683 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"6ec4683\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"688\" height=\"888\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" class=\"attachment-large size-large wp-image-63589\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?w=688&amp;ssl=1 688w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?resize=232%2C300&amp;ssl=1 232w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?resize=58%2C75&amp;ssl=1 58w\" sizes=\"(max-width:767px) 688px, 688px\" data-attachment-id=\"63589\" data-permalink=\"https:\/\/rheolution.com\/fr\/tg-effect-tand-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" data-orig-size=\"688,888\" data-comments-opened=\"1\" data-image-title=\"tg effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-TanD-Bar-graph.png?fit=688%2C888&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f788b5a elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f788b5a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 6.<\/b><span style=\"font-weight: 400;\">  Propri\u00e9t\u00e9s visco\u00e9lastiques \u00e0 l&rsquo;\u00e9tat final des hydrogels de g\u00e9latine r\u00e9ticul\u00e9s par la transglutaminase \u00e0 45 \u00b0C. Le module de stockage en cisaillement (G\u2032, \u00e0 gauche), le module de perte en cisaillement (G\u2033, au centre), et le facteur d&rsquo;amortissement (tan \u03b4, \u00e0 droite) ont \u00e9t\u00e9 calcul\u00e9s \u00e0 partir de la zone de plateau (5 \u00e0 6 h) des courbes de g\u00e9lification d\u2019hydrogels de g\u00e9latine \u00e0 10 % en masse r\u00e9ticul\u00e9s avec 30 U g\u207b\u00b9 et 15 U g\u207b\u00b9 de transglutaminase. Les barres indiquent la moyenne \u00b1 l\u2019\u00e9cart-type, les points de donn\u00e9es individuels \u00e9tant superpos\u00e9s. La signification statistique est indiqu\u00e9e comme suit : ***p &lt; 0,001 ; ns, non significatif.   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-989a981 e-grid e-con-full e-con e-child\" data-id=\"989a981\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-108df9d elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"108df9d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"705\" height=\"926\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" class=\"attachment-large size-large wp-image-63581\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?w=705&amp;ssl=1 705w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=228%2C300&amp;ssl=1 228w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=57%2C75&amp;ssl=1 57w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?resize=700%2C919&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, 705px\" data-attachment-id=\"63581\" data-permalink=\"https:\/\/rheolution.com\/fr\/tg-effect-sol-gel-transition-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" data-orig-size=\"705,926\" data-comments-opened=\"1\" data-image-title=\"tg effect- sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-sol-gel-transition.png?fit=705%2C926&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53efe65 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"53efe65\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"663\" height=\"942\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" class=\"attachment-large size-large wp-image-63597\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?w=663&amp;ssl=1 663w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?resize=211%2C300&amp;ssl=1 211w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?resize=53%2C75&amp;ssl=1 53w\" sizes=\"(max-width:767px) 663px, 663px\" data-attachment-id=\"63597\" data-permalink=\"https:\/\/rheolution.com\/fr\/tg-effect-time-to-plateau-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" data-orig-size=\"663,942\" data-comments-opened=\"1\" data-image-title=\"tg effect- Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/tg-effect-Time-to-plateau.png?fit=663%2C942&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-26f2837 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"26f2837\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 7.<\/b><span style=\"font-weight: 400;\">  Temps de g\u00e9lification, d\u00e9fini par le point d\u2019intersection G\u2032\u2013G\u2033 (\u00e0 gauche), et temps n\u00e9cessaire pour atteindre le plateau visco\u00e9lastique (\u00e0 droite ; temps n\u00e9cessaire pour que G\u2032 atteigne 90 % de sa valeur finale stabilis\u00e9e) pour une g\u00e9latine \u00e0 10 % en poids r\u00e9ticul\u00e9e par la transglutaminase (\u00e0 45 \u00b0C) \u00e0 des concentrations de 30 U g\u207b\u00b9 et 15 U g\u207b\u00b9. Les barres repr\u00e9sentent la moyenne \u00b1 l\u2019\u00e9cart-type, les r\u00e9plicats individuels \u00e9tant superpos\u00e9s. La signification statistique est indiqu\u00e9e par *p &lt; 0,05.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7af856 elementor-widget elementor-widget-heading\" data-id=\"b7af856\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Effet de la temp\u00e9rature sur la g\u00e9lification et les propri\u00e9t\u00e9s m\u00e9caniques<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afd54d4 elementor-widget elementor-widget-text-editor\" data-id=\"afd54d4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">\u00c0 l&rsquo;instar des r\u00e9sultats pr\u00e9c\u00e9dents, les figures 8, 9 et 10 pr\u00e9sentent l\u2019\u00e9volution dans le temps de G\u2032 au cours de la r\u00e9ticulation de la g\u00e9latine, les valeurs finales de G\u2032 et de G\u2033, ainsi que le temps n\u00e9cessaire pour atteindre le point de transition et le plateau, cette fois-ci pour deux conditions diff\u00e9rant par leur temp\u00e9rature d\u2019essai (37 \u00b0C et 45 \u00b0C). Bien qu\u2019une l\u00e9g\u00e8re diff\u00e9rence ait \u00e9t\u00e9 observ\u00e9e dans la cin\u00e9tique de r\u00e9ticulation, aucune diff\u00e9rence statistiquement significative n\u2019a \u00e9t\u00e9 constat\u00e9e pour aucun des param\u00e8tres \u00e9valu\u00e9s.   <\/span><\/p><p><span style=\"font-weight: 400;\">Il convient de noter qu\u2019\u00e0 37 \u00b0C, il n\u2019a pas \u00e9t\u00e9 possible de d\u00e9terminer le temps n\u00e9cessaire pour atteindre le point de g\u00e9lification pour deux des trois \u00e9chantillons, la g\u00e9lification s\u2019\u00e9tant d\u00e9j\u00e0 produite avant le d\u00e9but de l\u2019essai. \u00c0 cette temp\u00e9rature, une g\u00e9lification thermique peut \u00e9galement se produire, ce qui pourrait acc\u00e9l\u00e9rer le processus de g\u00e9lification et expliquer ce r\u00e9sultat. Par cons\u00e9quent, il peut exister une diff\u00e9rence statistiquement significative concernant ce param\u00e8tre (temps de g\u00e9lification) entre ces deux conditions (37 \u00b0C par rapport \u00e0 45 \u00b0C).    <\/span><\/p><p><span style=\"font-weight: 400;\">En ce qui concerne les applications biom\u00e9dicales, les r\u00e9sultats indiquent que ces formulations peuvent \u00eatre trait\u00e9es \u00e0 des temp\u00e9ratures physiologiques tout en conservant une activit\u00e9 enzymatique et des propri\u00e9t\u00e9s visco\u00e9lastiques finales similaires, ce qui constitue un avantage pour leur utilisation pratique.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-56855da elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"56855da\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"655\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1024%2C655&amp;ssl=1\" class=\"attachment-large size-large wp-image-63641\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?w=1500&amp;ssl=1 1500w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=300%2C192&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=1024%2C655&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=768%2C491&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=117%2C75&amp;ssl=1 117w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?resize=700%2C448&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:1024px) 100vw, 1024px\" data-attachment-id=\"63641\" data-permalink=\"https:\/\/rheolution.com\/fr\/15-5-compare-temp-g-kinetics-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1500%2C959&amp;ssl=1\" data-orig-size=\"1500,959\" data-comments-opened=\"1\" data-image-title=\"15%, 5% compare temp &amp;#8211; G&amp;rsquo; kinetics\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/15-5-compare-temp-G-kinetics.png?fit=1024%2C655&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-afc49b7 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"afc49b7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 8.<\/b><span style=\"font-weight: 400;\">  \u00c9volution du module de stockage en cisaillement (G\u2032) au cours de la g\u00e9lification d&rsquo;hydrogels de g\u00e9latine \u00e0 15 % en masse, r\u00e9ticul\u00e9s par la transglutaminase (30 U g\u207b\u00b9) \u00e0 37 \u00b0C et 45 \u00b0C.<\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1eb37cc e-grid e-con-full e-con e-child\" data-id=\"1eb37cc\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-47cba32 elementor-widget elementor-widget-image\" data-id=\"47cba32\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"867\" height=\"750\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" class=\"attachment-large size-large wp-image-63657\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?w=867&amp;ssl=1 867w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=300%2C260&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=768%2C664&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=87%2C75&amp;ssl=1 87w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?resize=700%2C606&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:867px) 100vw, 867px\" data-attachment-id=\"63657\" data-permalink=\"https:\/\/rheolution.com\/fr\/temp-effect-g-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" data-orig-size=\"867,750\" data-comments-opened=\"1\" data-image-title=\"temp effect &amp;#8211; G&amp;rsquo; Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-G-Bar-graph.png?fit=867%2C750&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bd60dae elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"bd60dae\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"784\" height=\"788\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" class=\"attachment-large size-large wp-image-63649\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?w=784&amp;ssl=1 784w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=298%2C300&amp;ssl=1 298w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=768%2C772&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=75%2C75&amp;ssl=1 75w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?resize=700%2C704&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:784px) 100vw, 784px\" data-attachment-id=\"63649\" data-permalink=\"https:\/\/rheolution.com\/fr\/g-temp-effect-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" data-orig-size=\"784,788\" data-comments-opened=\"1\" data-image-title=\"g\u00a0\u00bb temp effect bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/g-temp-effect-bar-graph.png?fit=784%2C788&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c4e05d8 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"c4e05d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"805\" height=\"750\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" class=\"attachment-large size-large wp-image-63633\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?w=805&amp;ssl=1 805w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=300%2C280&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=768%2C716&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=81%2C75&amp;ssl=1 81w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?resize=700%2C652&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:805px) 100vw, 805px\" data-attachment-id=\"63633\" data-permalink=\"https:\/\/rheolution.com\/fr\/temp-effect-tand-bar-graph-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" data-orig-size=\"805,750\" data-comments-opened=\"1\" data-image-title=\"temp effect- TanD Bar graph\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-TanD-Bar-graph.png?fit=805%2C750&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e09e2fc elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"e09e2fc\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 9.<\/b><span style=\"font-weight: 400;\">  Propri\u00e9t\u00e9s visco\u00e9lastiques finales des hydrogels de g\u00e9latine r\u00e9ticul\u00e9s par la transglutaminase \u00e0 37 \u00b0C et 45 \u00b0C. Le module de stockage en cisaillement (G\u2032, \u00e0 gauche), le module de perte de cisaillement (G\u2033, au centre) et le facteur d&rsquo;amortissement (tan \u03b4, \u00e0 droite) ont \u00e9t\u00e9 calcul\u00e9s \u00e0 partir de la zone de plateau (5 \u00e0 6 h) des courbes de g\u00e9lification d\u2019hydrogels de g\u00e9latine \u00e0 15 % en masse r\u00e9ticul\u00e9s avec 30 U g\u207b\u00b9 de transglutaminase. Les barres indiquent la moyenne \u00b1 l\u2019\u00e9cart-type, avec superposition des points de donn\u00e9es individuels. La signification statistique est indiqu\u00e9e par \u00ab ns \u00bb (non significatif).   <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c25765a e-grid e-con-full e-con e-child\" data-id=\"c25765a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2b74930 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"2b74930\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"871\" height=\"771\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" class=\"attachment-large size-large wp-image-63625\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?w=871&amp;ssl=1 871w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=300%2C266&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=768%2C680&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=85%2C75&amp;ssl=1 85w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?resize=700%2C620&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:871px) 100vw, 871px\" data-attachment-id=\"63625\" data-permalink=\"https:\/\/rheolution.com\/fr\/temp-effect-sol-gel-transition-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" data-orig-size=\"871,771\" data-comments-opened=\"1\" data-image-title=\"temp effect- sol-gel transition\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-sol-gel-transition.png?fit=871%2C771&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-562ab31 elementor-widget__width-initial elementor-widget elementor-widget-image\" data-id=\"562ab31\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"780\" height=\"780\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" class=\"attachment-large size-large wp-image-63617\" alt=\"\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?w=780&amp;ssl=1 780w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=300%2C300&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=150%2C150&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=768%2C768&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=75%2C75&amp;ssl=1 75w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?resize=700%2C700&amp;ssl=1 700w\" sizes=\"(max-width:767px) 700px, (max-width:780px) 100vw, 780px\" data-attachment-id=\"63617\" data-permalink=\"https:\/\/rheolution.com\/fr\/temp-effect-min-time-to-plateau-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" data-orig-size=\"780,780\" data-comments-opened=\"1\" data-image-title=\"temp effect min- Time to plateau\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/temp-effect-min-Time-to-plateau.png?fit=780%2C780&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bc7f5a7 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"bc7f5a7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><em><b>Figure 10.<\/b><span style=\"font-weight: 400;\">  Temps de g\u00e9lification, d\u00e9fini par le croisement G\u2032\u2013G\u2033 (\u00e0 gauche), et temps n\u00e9cessaire pour atteindre le plateau visco\u00e9lastique (\u00e0 droite ; temps n\u00e9cessaire pour que G\u2032 atteigne 90 % de sa valeur finale stabilis\u00e9e) pour une g\u00e9latine \u00e0 15 % en poids r\u00e9ticul\u00e9e par la transglutaminase (30 U g\u207b\u00b9) \u00e0 37 \u00b0C et 45 \u00b0C. Les barres repr\u00e9sentent la moyenne \u00b1 l&rsquo;\u00e9cart-type, les r\u00e9plicats individuels \u00e9tant superpos\u00e9s. La signification statistique est indiqu\u00e9e par \u00ab ns \u00bb, qui signifie \u00ab non significatif \u00bb.  <\/span><\/em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8ad5968 elementor-widget elementor-widget-heading\" data-id=\"8ad5968\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Conclusions et perspectives<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7d7a98 elementor-widget elementor-widget-text-editor\" data-id=\"b7d7a98\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Cette \u00e9tude d\u00e9montre que les propri\u00e9t\u00e9s m\u00e9caniques des hydrogels de g\u00e9latine et leur cin\u00e9tique de g\u00e9lification peuvent \u00eatre ajust\u00e9es avec pr\u00e9cision gr\u00e2ce \u00e0 des param\u00e8tres de formulation, puis suivies quantitativement en temps r\u00e9el \u00e0 l&rsquo;aide d&rsquo;ElastoSens\u2122 Bio. Il est important de noter que la r\u00e9ticulation enzymatique constitue une strat\u00e9gie efficace pour stabiliser les hydrogels \u00e0 base de g\u00e9latine \u00e0 temp\u00e9rature physiologique, ce qui permet de surmonter les limites des gels physiques thermiquement r\u00e9versibles et d\u2019utiliser ces hydrogels dans des conditions biologiquement pertinentes. La capacit\u00e9 \u00e0 r\u00e9aliser une r\u00e9ticulation enzymatique \u00e0 temp\u00e9rature physiologique tout en obtenant des propri\u00e9t\u00e9s m\u00e9caniques reproductibles et contr\u00f4lables souligne la pertinence de cette plateforme pour le d\u00e9veloppement de biomat\u00e9riaux, le criblage de formulations et le contr\u00f4le qualit\u00e9.  <\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9383078 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"9383078\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Slides\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-c97f30c swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"elastosens2\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8cce0c6 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"8cce0c6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>D\u00e9couvrez comment notre technologie mesure de mani\u00e8re non destructive les propri\u00e9t\u00e9s visco\u00e9lastiques des biomat\u00e9riaux mous et des tissus en utilisant des micro-volumes d\u2019\u00e9chantillons<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c4ce1d0 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"c4ce1d0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/fr\/contact\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Contactez-nous pour plus d'informations<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-efbb293 elementor-widget elementor-widget-heading\" data-id=\"efbb293\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Articles connexes<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f623a9 premium-blog-cta-full-yes premium-floating-effects-yes premium-disable-fe-yes elementor-widget__width-initial premium-blog-align-left elementor-widget elementor-widget-premium-addon-blog\" data-id=\"3f623a9\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;force_height&quot;:&quot;true&quot;,&quot;premium_blog_grid&quot;:&quot;yes&quot;,&quot;premium_blog_layout&quot;:&quot;even&quot;,&quot;premium_blog_columns_number&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_tablet&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_mobile&quot;:&quot;100%&quot;,&quot;scroll_to_offset&quot;:&quot;yes&quot;,&quot;premium_fe_trigger&quot;:&quot;load&quot;,&quot;premium_fe_direction&quot;:&quot;alternate&quot;,&quot;premium_fe_loop&quot;:&quot;default&quot;,&quot;premium_fe_easing&quot;:&quot;easeInOutSine&quot;}\" data-widget_type=\"premium-addon-blog.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t\n\n\t\t\t\t<div class=\"premium-blog-wrap  premium-blog-even\" data-page=\"70904\" data-pagination=\"true\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7000\" height=\"2322\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?fit=7000%2C2322&amp;ssl=1\" class=\"attachment-full size-full wp-image-63000\" alt=\"\" data-attachment-id=\"63000\" data-permalink=\"https:\/\/rheolution.com\/fr\/fibrin-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?fit=7000%2C2322&amp;ssl=1\" data-orig-size=\"7000,2322\" data-comments-opened=\"1\" data-image-title=\"FIBRIN\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?w=7000&amp;ssl=1 7000w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=1536%2C510&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/FIBRIN.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7000px) 100vw, 7000px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-de-fibrine\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hydrogels de fibrine<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-de-fibrine\/\" target=\"_blank\">\n\t\t\t\tHydrogels de fibrine\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Les hydrogels de fibrine sont des biomat\u00e9riaux naturels \u00e0 base de prot\u00e9ines, form\u00e9s \u00e0 partir de la fibrine, ce polym\u00e8re insoluble g\u00e9n\u00e9r\u00e9 lors de la coagulation sanguine. La fibrine provient du fibrinog\u00e8ne, une glycoprot\u00e9ine plasmatique compos\u00e9e de trois paires de cha\u00eenes polypeptidiques qui s\u2019assemblent en un r\u00e9seau fibreux sous l\u2019effet d\u2019une activation enzymatique. Dans des conditions physiologiques, la formation de la fibrine est d\u00e9clench\u00e9e par le clivage du fibrinog\u00e8ne sous l\u2019action de la thrombine, ce qui expose des sites de polym\u00e9risation qui favorisent l\u2019auto-assemblage spontan\u00e9 en une matrice hydrat\u00e9e et poreuse.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-de-fibrine\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Lire la suite \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7000\" height=\"2322\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=7000%2C2322&amp;ssl=1\" class=\"attachment-full size-full wp-image-63128\" alt=\"\" data-attachment-id=\"63128\" data-permalink=\"https:\/\/rheolution.com\/fr\/alginate-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=7000%2C2322&amp;ssl=1\" data-orig-size=\"7000,2322\" data-comments-opened=\"1\" data-image-title=\"ALGINATE\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=7000&amp;ssl=1 7000w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=1536%2C510&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/ALGINATE-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7000px) 100vw, 7000px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-dalginate\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hydrogels d&rsquo;alginate<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-dalginate\/\" target=\"_blank\">\n\t\t\t\tHydrogels d&rsquo;alginate\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Les hydrogels d'alginate sont des r\u00e9seaux polym\u00e8res tridimensionnels riches en eau, d\u00e9riv\u00e9s de l'alginate, un polysaccharide anionique d'origine naturelle. L'alginate est principalement extrait des parois cellulaires des algues brunes appartenant \u00e0 la classe des ph\u00e9ophyc\u00e9es, bien qu'il puisse \u00e9galement \u00eatre biosynth\u00e9tis\u00e9 par certaines esp\u00e8ces bact\u00e9riennes telles que les Azotobacter et les Pseudomonas. Sur le plan structurel, l\u2019alginate est un copolym\u00e8re lin\u00e9aire compos\u00e9 d\u2019acide \u03b2-D-mannuronique (unit\u00e9s M) et d\u2019acide \u03b1-L-guluronique (unit\u00e9s G), reli\u00e9s par des liaisons glycosidiques 1\u21924.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/fr\/notes-application\/hydrogels-dalginate\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Lire la suite \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7875\" height=\"2612\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=7875%2C2612&amp;ssl=1\" class=\"attachment-full size-full wp-image-63420\" alt=\"\" data-attachment-id=\"63420\" data-permalink=\"https:\/\/rheolution.com\/fr\/gelatin-methacrylate-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=7875%2C2612&amp;ssl=1\" data-orig-size=\"7875,2612\" data-comments-opened=\"1\" data-image-title=\"Gelatin Methacrylate\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=7875&amp;ssl=1 7875w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=1536%2C509&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Gelatin-Methacrylate-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7875px) 100vw, 7875px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/fr\/notes-application\/gelma-hydrogels\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Hydrogels GelMA<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/fr\/notes-application\/gelma-hydrogels\/\" target=\"_blank\">\n\t\t\t\tHydrogels GelMA\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">La g\u00e9latine m\u00e9thacryloyle (GelMA) est un hydrogel photor\u00e9ticulable d\u00e9riv\u00e9 de la g\u00e9latine, elle-m\u00eame obtenue par hydrolyse partielle du collag\u00e8ne, la principale prot\u00e9ine structurelle de la matrice extracellulaire (MEC). Le GelMA est synth\u00e9tis\u00e9 par r\u00e9action de la g\u00e9latine avec de l'anhydride m\u00e9thacrylique, ce qui permet d'introduire des groupes fonctionnels m\u00e9thacryloyle sur les r\u00e9sidus d'acides amin\u00e9s de la g\u00e9latine tout en pr\u00e9servant la plupart de ses motifs bioactifs natifs.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/fr\/notes-application\/gelma-hydrogels\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Lire la suite \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"3\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"7875\" height=\"2612\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=7875%2C2612&amp;ssl=1\" class=\"attachment-full size-full wp-image-63388\" alt=\"\" data-attachment-id=\"63388\" data-permalink=\"https:\/\/rheolution.com\/fr\/polydimethylsiloxane-pdms-2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=7875%2C2612&amp;ssl=1\" data-orig-size=\"7875,2612\" data-comments-opened=\"1\" data-image-title=\"Polydimethylsiloxane (PDMS)\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?fit=1024%2C340&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=7875&amp;ssl=1 7875w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=300%2C100&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=1024%2C340&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=768%2C255&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=1536%2C509&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=2048%2C679&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=150%2C50&amp;ssl=1 150w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?resize=700%2C232&amp;ssl=1 700w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=2440&amp;ssl=1 2440w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Polydimethylsiloxane-PDMS-1.jpg?w=3660&amp;ssl=1 3660w\" sizes=\"(max-width:767px) 700px, (max-width:7875px) 100vw, 7875px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/fr\/notes-application\/polymeres-pdms\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Polym\u00e8res PDMS<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/fr\/notes-application\/polymeres-pdms\/\" target=\"_blank\">\n\t\t\t\tPolym\u00e8res PDMS\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">Le polydim\u00e9thylsiloxane (PDMS) est un polym\u00e8re polysiloxane synth\u00e9tique compos\u00e9 d\u2019un squelette siloxane inorganique souple (\u2013Si\u2013O\u2013Si\u2013) comportant des groupes lat\u00e9raux m\u00e9thyle. Cette architecture inorganique-organique unique conf\u00e8re au PDMS une mobilit\u00e9 de cha\u00eene exceptionnelle, une tr\u00e8s basse temp\u00e9rature de transition vitreuse et un comportement \u00e9lastom\u00e8re stable sur une large plage de temp\u00e9ratures. Le PDMS est produit exclusivement par synth\u00e8se industrielle, impliquant g\u00e9n\u00e9ralement l\u2019hydrolyse et la condensation de chlorosilanes, suivies d\u2019une polym\u00e9risation par ouverture de cycle de siloxanes cycliques.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/fr\/notes-application\/polymeres-pdms\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Lire la suite \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t<div class=\"premium-blog-footer\">\n\t\t\t\t\t\t\t<nav class=\"premium-blog-pagination-container\" role=\"navigation\" aria-label=\"Pagination\">\n\t\t\t\t<span aria-current=\"page\" class=\"page-numbers current\">1<\/span>\n<a class=\"page-numbers\" href=\"https:\/\/rheolution.com\/fr\/wp-json\/wp\/v2\/posts\/70904\/page\/2\/\">2<\/a>\n<a class=\"page-numbers\" href=\"https:\/\/rheolution.com\/fr\/wp-json\/wp\/v2\/posts\/70904\/page\/3\/\">3<\/a>\n<a class=\"next page-numbers\" href=\"https:\/\/rheolution.com\/fr\/wp-json\/wp\/v2\/posts\/70904\/page\/2\/\">Suivant \u00bb<\/a>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>La g\u00e9latine est un biopolym\u00e8re largement utilis\u00e9 dans le domaine des biomat\u00e9riaux, car elle est transformable dans l&rsquo;eau, biocompatible et capable de former des r\u00e9seaux souples et hydrat\u00e9s. Cependant, les gels de g\u00e9latine physiques peuvent s&rsquo;affaiblir ou fondre \u00e0 des temp\u00e9ratures proches de la temp\u00e9rature physiologique ; c&rsquo;est pourquoi on recourt couramment \u00e0 la r\u00e9ticulation covalente pour am\u00e9liorer leur stabilit\u00e9 thermique et leur int\u00e9grit\u00e9 m\u00e9canique. La g\u00e9latine X-Pure Gelatin\u00ae est une g\u00e9latine de haute qualit\u00e9, de qualit\u00e9 pharmaceutique, qui se caract\u00e9rise par des normes de puret\u00e9 rigoureuses et des performances constantes.  <\/p>\n","protected":false},"author":195401359,"featured_media":63492,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[285,296],"tags":[89,91,124,88],"class_list":["post-70904","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-notes-application","category-application-formulation-of-hydrogels","tag-elastosens-bio","tag-hydrogels","tag-life-sciences","tag-mechanical-testing"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.7 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>R\u00e9ticulation de la G\u00e9latine \u00e0 l&#039;aide de Transglutaminase Microbienne<\/title>\n<meta name=\"description\" content=\"\u00c9tude approfondie de la cin\u00e9tique de r\u00e9ticulation et du comportement visco\u00e9lastique des hydrogels de g\u00e9latine \u00e0 l&#039;aide de la transglutaminase microbienne.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/rheolution.com\/fr\/notes-application\/reticulation-de-la-gelatine-par-la-transglutaminase-microbienne\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"R\u00e9ticulation de la G\u00e9latine \u00e0 l&#039;aide de Transglutaminase Microbienne\" \/>\n<meta property=\"og:description\" content=\"\u00c9tude approfondie de la cin\u00e9tique de r\u00e9ticulation et du comportement visco\u00e9lastique des hydrogels de g\u00e9latine \u00e0 l&#039;aide de la transglutaminase microbienne.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/rheolution.com\/fr\/notes-application\/reticulation-de-la-gelatine-par-la-transglutaminase-microbienne\/\" \/>\n<meta property=\"og:site_name\" content=\"Rheolution\" \/>\n<meta property=\"article:published_time\" content=\"2026-02-03T21:34:19+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-08T19:59:10+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/rheolution.com\/wp-content\/uploads\/Gelatin-Crosslinking.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1536\" \/>\n\t<meta property=\"og:image:height\" content=\"580\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Ana Paula Knorst\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta 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