{"id":70939,"date":"2026-09-29T13:55:26","date_gmt":"2026-09-29T17:55:26","guid":{"rendered":"https:\/\/rheolution.com\/?p=70939"},"modified":"2026-09-29T13:55:26","modified_gmt":"2026-09-29T17:55:26","slug":"medir-el-crecimiento-de-la-levadura-en-un-biorreactor-mediante-la-turbidez","status":"publish","type":"post","link":"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-crecimiento-de-la-levadura-en-un-biorreactor-mediante-la-turbidez\/","title":{"rendered":"\u00bfC\u00f3mo se controla el crecimiento de la levadura en un biorreactor con TURBIDI.T\u2122?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"70939\" class=\"elementor elementor-70939 elementor-29296\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7eee7ee e-flex e-con-boxed e-con e-parent\" data-id=\"7eee7ee\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ea1afcb elementor-widget elementor-widget-heading\" data-id=\"ea1afcb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Nota de aplicaci\u00f3n | TURBIDI.T\u2122<\/h6>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2141c30 elementor-widget elementor-widget-heading\" data-id=\"2141c30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Avances en el seguimiento del crecimiento de la levadura mediante la medici\u00f3n de la turbidez\n<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e230945 elementor-widget elementor-widget-text-editor\" data-id=\"e230945\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"raw_components--panel--3IcXg inspect_panels--inspectionPanel--3Wboz\" tabindex=\"-1\"><div class=\"inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--propertyRowContent--7Y-mu inspect_panels--copyableRow--2_PiZ inspect_panels--highlightRow--21P9S\"><div class=\"inspect_panels--contentProperty--2Z1QI text--fontPos11--RSei3 text--_fontBase--YWDo0 ellipsis--ellipsisAfter8Lines--2rn32 ellipsis--_ellipsisAfterNLines--26JkZ\"><p>por <a href=\"https:\/\/www.linkedin.com\/in\/gloriapinilla\/\" target=\"_blank\" rel=\"noopener\">Gloria Pinilla<\/a> <span style=\"font-weight: 400;\">y <a href=\"https:\/\/www.linkedin.com\/in\/nora-kasaplar\" target=\"_blank\" rel=\"noopener\">Nora Kasaplar<\/a><\/span>,<\/p><p><span style=\"font-weight: 400;\">Aplicaci\u00f3n<\/span><span style=\"font-weight: 400;\"> Cient\u00edficos,<\/span> <a href=\"https:\/\/www.linkedin.com\/company\/1168758\" target=\"_blank\" rel=\"noopener\">Rheolution Inc.<\/a><\/p><p>En colaboraci\u00f3n con  <\/p><p><em>Escuela Nacional Superior de Tecnolog\u00eda de Biomol\u00e9culas de Burdeos (ENSTBB).<\/em><\/p><p><span id=\"docs-internal-guid-f0b2c698-7fff-5eae-53f6-94e076f7192c\" style=\"font-weight: normal;\"><span style=\"font-size: 10pt; font-family: Verdana, sans-serif; color: #002060; background-color: transparent; font-weight: 400; font-style: normal; font-variant: normal; white-space-collapse: preserve;\"><span style=\"border-width: initial; border-style: none; display: inline-block; overflow: hidden; width: 183px; height: 59px;\"><a href=\"https:\/\/enstbb.bordeaux-inp.fr\/fr\" target=\"_blank\" rel=\"noopener\"><img decoding=\"async\" class=\"alignnone\" src=\"https:\/\/lh6.googleusercontent.com\/taVq6fxwO3y9M_Onk2MznWVYK4fGUswV4ylXbLTXiz--6Lnb5y_D8UU_0-BYZQ84LvhP4-Wzx6Je0H-TEH850xcAVrlCU1qZoUZuH2Wo37mQLyWOFqe-pn1FNScC6b94yI6cUA5K2fY2Yom1K9-EEAQ\" alt=\"\" width=\"183\" height=\"59\"><\/a><\/span><\/span><\/span><span style=\"letter-spacing: 0px;\"><br><\/span><\/p><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-35f268f elementor-widget__width-initial elementor-widget elementor-widget-heading\" data-id=\"35f268f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resumen<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb634db elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-text-editor\" data-id=\"bb634db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>El seguimiento del crecimiento de la levadura es un proceso clave para garantizar unas condiciones \u00f3ptimas y la productividad en los procesos biotecnol\u00f3gicos.  <\/li><li>Las mediciones basadas en la turbidez se han convertido en un m\u00e9todo r\u00e1pido y no destructivo para estimar el crecimiento de la levadura y hacer un seguimiento de la densidad del cultivo a lo largo del tiempo. Se ha utilizado <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> (Rheolution, QC, Canad\u00e1) para supervisar con precisi\u00f3n el crecimiento de <i>S. cerevisiae<\/i> a lo largo del tiempo.<\/li><li>Se han comparado los resultados de turbidez (850 nm) y absorbancia (600 nm), lo que ha puesto de manifiesto tendencias similares y una fuerte correlaci\u00f3n.<\/li><li><a href=\"https:\/\/rheolution.com\/life-sciences\/products\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> ha demostrado tener m\u00faltiples ventajas frente al espectrofot\u00f3metro en lo que respecta al an\u00e1lisis del crecimiento celular y la recopilaci\u00f3n de datos.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9453c51 e-flex e-con-boxed e-con e-parent\" data-id=\"9453c51\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5bdb09e elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"5bdb09e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Avances en el seguimiento del crecimiento de la levadura: medici\u00f3n de la turbidez<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5965530 elementor-widget elementor-widget-heading\" data-id=\"5965530\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Introducci\u00f3n<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b5ece66 elementor-widget elementor-widget-text-editor\" data-id=\"b5ece66\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>En el \u00e1mbito de la bioingenier\u00eda y la biotecnolog\u00eda, las c\u00e9lulas act\u00faan como catalizadores para la producci\u00f3n de diversos productos valiosos, como biocombustibles, f\u00e1rmacos, anticuerpos, enzimas industriales y productos qu\u00edmicos de origen biol\u00f3gico [1]. En este contexto, la levadura, un microorganismo muy utilizado, desempe\u00f1a un papel fundamental. Controlar el crecimiento de la levadura es de vital importancia para garantizar unas condiciones \u00f3ptimas y la productividad en los procesos biotecnol\u00f3gicos [2].  <\/p><p>Tradicionalmente, el an\u00e1lisis espectrofotom\u00e9trico se ha utilizado para evaluar la densidad \u00f3ptica a 600 nm (DO600nm) midiendo la absorci\u00f3n de luz por parte del cultivo de levadura, y para seguir la evoluci\u00f3n de la turbidez de una poblaci\u00f3n celular. Las mediciones basadas en la turbidez se han convertido en un m\u00e9todo r\u00e1pido y no destructivo para estimar el crecimiento de la levadura y seguir la densidad del cultivo a lo largo del tiempo. Esta metodolog\u00eda se basa en los principios de la dispersi\u00f3n de la luz, seg\u00fan los cuales los cambios en la concentraci\u00f3n y el tama\u00f1o de las c\u00e9lulas influyen en la intensidad de la luz dispersada por el cultivo. Al cuantificar la turbidez resultante, se puede obtener informaci\u00f3n valiosa sobre la progresi\u00f3n del crecimiento celular.   <\/p><p><em>La Saccharomyces cerevisiae<\/em> (5-10 \u00b5m), tambi\u00e9n conocida como levadura de panader\u00eda o levadura de cerveza, ocupa un lugar destacado como organismo modelo en el \u00e1mbito de la producci\u00f3n industrial. Esto se debe a que est\u00e1 muy bien caracterizada, es resistente, tiene una alta productividad y es muy vers\u00e1til.   <\/p><p>Esta nota de aplicaci\u00f3n se centra en el uso de <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\">TURBIDI.T\u2122<\/a> (Rheolution, QC, Canad\u00e1) para monitorizar el crecimiento de <em>S. cerevisiae<\/em> a lo largo del tiempo. Los resultados obtenidos se comparan y se correlacionan con las mediciones de DO600 nm realizadas con un espectrofot\u00f3metro est\u00e1ndar. Al analizar esta comparaci\u00f3n, se pueden obtener datos valiosos para comprender mejor la eficacia y la fiabilidad de <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\">TURBIDI.T\u2122<\/a> como herramienta de seguimiento del crecimiento de la levadura.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-62f3970 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"62f3970\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Seguimiento del crecimiento de la levadura mediante la medici\u00f3n de la turbidez: un estudio de caso sobre S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-978e221 elementor-widget elementor-widget-heading\" data-id=\"978e221\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Materiales y m\u00e9todos<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-299f474 elementor-widget elementor-widget-text-editor\" data-id=\"299f474\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Se prepar\u00f3 un precultivo de levadura de 100 ml en condiciones est\u00e9riles inoculando colonias <em>de S. cerevisiae<\/em> procedentes de un cultivo s\u00f3lido en una placa de Petri, y se dej\u00f3 crecer durante toda la noche. Al mismo tiempo, se prepar\u00f3 un biorreactor de 3 l (<a href=\"https:\/\/www.sartorius.com\/en\/products\/fermentation-bioreactors\/benchtop-bioreactors\/biostat-b?_gl=1*dzzxof*_up*MQ..&#038;gclid=CjwKCAjw-7OlBhB8EiwAnoOEkyL2kNBymV2yGELc9KSmyLWW_BODZo5jZrEa5-9mE9bDhvznnIe9FBoCDHsQAvD_BwE\">BIOSTAT B; Sartorius Stedim Biotech, Gotinga, Alemania<\/a>) que conten\u00eda medio de cultivo sint\u00e9tico est\u00e9ril [3] se prepar\u00f3 para esterilizarlo durante toda la noche en un autoclave. El medio de cultivo sint\u00e9tico estaba compuesto por diversas sales inorg\u00e1nicas ((NH\u2084)\u2082SO\u2084, KH\u2082PO\u2084, MgSO\u2084), vitaminas, oligoelementos y glucosa. Tras la esterilizaci\u00f3n, se calibraron las sondas de pH y pO\u2082, as\u00ed como la bomba de alimentaci\u00f3n de glucosa, siguiendo las instrucciones del software del BIOSTAT B (<strong>Figura 1<\/strong>).     <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8c2bea5 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"8c2bea5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1604\" height=\"1024\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" class=\"attachment-full size-full wp-image-29333\" alt=\"Yeast bioreactor in sterile conditions.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?w=1604&amp;ssl=1 1604w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=300%2C192&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1024%2C654&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=768%2C490&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1536%2C981&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=1200%2C766&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=229%2C146&amp;ssl=1 229w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=50%2C32&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?resize=117%2C75&amp;ssl=1 117w\" sizes=\"(max-width:767px) 700px, (max-width:1604px) 100vw, 1604px\" data-attachment-id=\"29333\" data-permalink=\"https:\/\/rheolution.com\/es\/fig1_biostat-software_merged\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1604%2C1024&amp;ssl=1\" data-orig-size=\"1604,1024\" data-comments-opened=\"1\" data-image-title=\"Yeast bioreactor\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig1_BIOSTAT-software_merged.jpg?fit=1024%2C654&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f611ec3 elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"f611ec3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 1: <\/b>Configuraci\u00f3n <b>del biorreactor <\/b>para el cultivo de levadura en condiciones est\u00e9riles.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9aba0ca elementor-widget elementor-widget-text-editor\" data-id=\"9aba0ca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Para calibrar el <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, se utilizaron patrones de formazina antes de realizar las pruebas. La emisi\u00f3n y la recepci\u00f3n de luz se activaron utilizando cartuchos Emitt.805 (longitud de onda de 850 nm) y cartuchos Receiv.ViS (longitud de onda comprendida entre 400 nm y 1000 nm), respectivamente. Decidimos usar el cartucho de 850 nm porque el efecto de la absorci\u00f3n de luz era menor. El espectrofot\u00f3metro (<a href=\"https:\/\/www.safas.com\/?SAFAS-UVmc1-spectrophotometer-the-price-and-use-of-a-single-beam-with-double-beam-quality&#038;lang=en\" target=\"_blank\" rel=\"noopener\">SAFAS UVmc, SAFAS, M\u00f3naco<\/a>) se calibr\u00f3 con un vial de agua destilada desionizada (ddH\u2082O) antes de cada medici\u00f3n.  <\/p><p>Las levaduras precultivadas se sembraron en el biorreactor a una densidad de 220 FTU (0,21 uOD). Despu\u00e9s, se control\u00f3 el crecimiento de la levadura tomando al\u00edcuotas cada hora durante 8 horas. Las mediciones de turbidez a 850 nm (expresadas en FTU) y de absorbancia (expresadas en uOD) se realizaron por triplicado para cada punto temporal (n = 3). Para las mediciones espectrofotom\u00e9tricas, la muestra del cultivo de levadura se diluy\u00f3 en una proporci\u00f3n de 1:100, mientras que para las mediciones turbidim\u00e9tricas no se necesit\u00f3 diluci\u00f3n alguna y las muestras se analizaron directamente. Los resultados se presentan como media \u00b1 desviaci\u00f3n est\u00e1ndar.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b2ad55a elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-heading\" data-id=\"b2ad55a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">An\u00e1lisis de la din\u00e1mica de crecimiento de la levadura: mediciones de turbidez frente a mediciones de absorbancia en cultivos de S. cerevisiae<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aadb984 elementor-widget elementor-widget-heading\" data-id=\"aadb984\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Resultados y discusi\u00f3n<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-99aa2a5 elementor-widget elementor-widget-text-editor\" data-id=\"99aa2a5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>La figura 2<\/b> muestra el patr\u00f3n de crecimiento de la levadura a lo largo del tiempo, con un notable aumento exponencial tanto en unidades de turbidez (FTU) como en unidades de absorbancia (uOD) tras un breve periodo de latencia de 2 horas. Durante la fase de latencia, las c\u00e9lulas de levadura pasan por un periodo de adaptaci\u00f3n, ajust\u00e1ndose poco a poco a su entorno. Al finalizar esta fase, la curva se dispara, lo que demuestra la proliferaci\u00f3n continua de las c\u00e9lulas de levadura. Esta fase de crecimiento exponencial indica que hay condiciones \u00f3ptimas para el crecimiento de la levadura, en las que factores como la disponibilidad de nutrientes y las se\u00f1ales ambientales favorecen la divisi\u00f3n celular activa y la actividad metab\u00f3lica.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bd7401d elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"bd7401d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"2827\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29324\" alt=\"Turbidity trend of S. cerevisiae culture over 8h, 850nm vs. OD600nm comparison graph\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2827&amp;ssl=1 2827w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=300%2C170&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1024%2C580&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=768%2C435&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1536%2C870&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=2048%2C1160&amp;ssl=1 2048w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=1200%2C680&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=258%2C146&amp;ssl=1 258w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=50%2C28&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?resize=132%2C75&amp;ssl=1 132w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?w=2440&amp;ssl=1 2440w\" sizes=\"(max-width:767px) 700px, (max-width:2827px) 100vw, 2827px\" data-attachment-id=\"29324\" data-permalink=\"https:\/\/rheolution.com\/es\/fig2_cell-grow-evolution\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=2827%2C1601&amp;ssl=1\" data-orig-size=\"2827,1601\" data-comments-opened=\"1\" data-image-title=\"Turbidity trend of S. cerevisiae\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig2_Cell-grow-evolution.png?fit=1024%2C580&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7801f2e elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"7801f2e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: bold; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">Figura 2:  <\/span><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\">Montaje de un biorreactor para el cultivo de levadura en condiciones est\u00e9riles. Evoluci\u00f3n de la turbidez de   <\/span><i style=\"font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; font-size: 16px; font-family: 'Red Hat Text', sans-serif;\">S. cerevisiae<\/i><span style=\"font-family: 'Red Hat Text', sans-serif; font-size: 16px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\"> cultivo durante 8 h y comparaci\u00f3n entre la turbidez a 850 nm y la DO<\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\"><span style=\"font-size: 0.6em; vertical-align: sub;\">600 nm<\/span><\/span><span style=\"font-style: normal; font-variant: normal; font-weight: 400; font-size: 10pt; font-family: Verdana, sans-serif; letter-spacing: 0px; white-space-collapse: preserve; background-color: transparent; color: #002060;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-52474e0 elementor-widget elementor-widget-text-editor\" data-id=\"52474e0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Las mediciones de turbidez obtenidas con <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\">TURBIDI.T\u2122<\/a> (representadas en el eje Y de la izquierda) coinciden pr\u00e1cticamente con los valores de absorbancia obtenidos con un espectrofot\u00f3metro est\u00e1ndar (que se muestran en el eje Y de la derecha), ya que ambas curvas siguen una tendencia similar. Las desviaciones est\u00e1ndar fueron todas muy bajas (las barras de error aparecen en el gr\u00e1fico), lo que demuestra la alta precisi\u00f3n de las mediciones (variabilidad inferior al 1 %). <\/p><p>En consonancia con lo que se ve en <strong>la figura 2<\/strong>, hay una correlaci\u00f3n lineal positiva entre la turbidez y las mediciones de OD600 nm, con un impresionante coeficiente de determinaci\u00f3n (R\u00b2) de 0,998 (<strong>figura 3<\/strong>). Este elevado valor de R\u00b2 indica una relaci\u00f3n s\u00f3lida y fiable entre la turbidez y la DO600 nm, lo que sugiere que las mediciones de turbidez pueden servir eficazmente como sustituto de las mediciones basadas en la absorbancia a la hora de controlar el crecimiento de la levadura. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-97440cd elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor-widget elementor-widget-image\" data-id=\"97440cd\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"2001\" height=\"1601\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" class=\"attachment-full size-full wp-image-29326\" alt=\"FTU (850 nm) vs. uOD (600 nm) Correlation in Cell Culture Growth Graph.\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?w=2001&amp;ssl=1 2001w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=300%2C240&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1024%2C819&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=768%2C614&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1536%2C1229&amp;ssl=1 1536w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=1200%2C960&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=182%2C146&amp;ssl=1 182w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=50%2C40&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?resize=94%2C75&amp;ssl=1 94w\" sizes=\"(max-width:767px) 700px, (max-width:2001px) 100vw, 2001px\" data-attachment-id=\"29326\" data-permalink=\"https:\/\/rheolution.com\/es\/fig3_correlation-between-ftu-and-uod\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=2001%2C1601&amp;ssl=1\" data-orig-size=\"2001,1601\" data-comments-opened=\"1\" data-image-title=\"Correlation in Cell Culture Growth\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Fig3_Correlation-between-FTU-and-uOD.png?fit=1024%2C819&amp;ssl=1\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ccd96b elementor-widget__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"1ccd96b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figura 3: Correlaci\u00f3n <\/b>entre la FTU (850 nm) y la uOD (600 nm) en la medici\u00f3n del crecimiento de cultivos celulares.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8a7ac8d elementor-widget elementor-widget-text-editor\" data-id=\"8a7ac8d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La estrecha correspondencia entre las mediciones de turbidez y de absorbancia, junto con el elevado coeficiente de correlaci\u00f3n, pone de manifiesto la eficacia y la precisi\u00f3n de las mediciones basadas en la turbidez realizadas con <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> para seguir la evoluci\u00f3n del crecimiento de la levadura.<\/p><p>Adem\u00e1s, la posibilidad de obtener datos fiables sobre el crecimiento sin necesidad de diluir las muestras, como ocurre en los an\u00e1lisis espectrofotom\u00e9tricos tradicionales, pone a\u00fan m\u00e1s de relieve las ventajas que ofrece el m\u00e9todo basado en la turbidez. En general, estos resultados demuestran el potencial de <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> como una herramienta valiosa para el seguimiento preciso y eficiente del crecimiento de la levadura en los bioprocesos, lo que contribuye a los avances en la investigaci\u00f3n y las aplicaciones biotecnol\u00f3gicas.   <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19ccca4 elementor-widget elementor-widget-heading\" data-id=\"19ccca4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Conclusiones y perspectivas<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a93e4fa elementor-widget elementor-widget-text-editor\" data-id=\"a93e4fa\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>El sistema <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> ha demostrado ser una herramienta potente y precisa para controlar el crecimiento y la cin\u00e9tica de los cultivos celulares en cultivos de levadura.  <\/li><li>La comparaci\u00f3n entre la turbidez y el valor OD600 nm revela una fuerte relaci\u00f3n lineal, lo que convierte a la turbidez en una alternativa precisa a las mediciones basadas en la absorbancia. Esto pone de manifiesto la eficacia de la turbidez a la hora de evaluar con precisi\u00f3n la din\u00e1mica de crecimiento de la levadura. <\/li><li><a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> ofrece varias ventajas en comparaci\u00f3n con el an\u00e1lisis con espectrofot\u00f3metro. Al no tener que diluir las muestras, gracias al amplio rango de valores de turbidez que mide el instrumento <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a>, se reducen los errores de medici\u00f3n y se ahorra un tiempo muy valioso. Adem\u00e1s, la tableta port\u00e1til facilita la recogida y el almacenamiento de datos, lo que mejora la comodidad y la accesibilidad.    <\/li><li>La versatilidad del sistema <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> permite utilizar diferentes viales para realizar mediciones en distintas configuraciones experimentales.<\/li><li>La escalabilidad del sistema <a href=\"https:\/\/rheolution.com\/es\/productos\/turbidi-t\/\" target=\"_blank\" rel=\"noopener\">TURBIDI.T\u2122<\/a> aumenta a\u00fan m\u00e1s su valor. Conectar varias unidades a una sola tableta de control mejora la eficiencia de los ensayos, lo que permite realizar mediciones simult\u00e1neas en experimentos a mayor escala. <\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d297cde elementor-align-center elementor-widget elementor-widget-button\" data-id=\"d297cde\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/wp-content\/uploads\/Microbiology_turbidity-1.pdf\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Accede a la nota completa de la aplicaci\u00f3n (PDF) <\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ee666c7 elementor-widget__width-initial elementor-widget-mobile__width-inherit elementor--h-position-center elementor--v-position-middle elementor-arrows-position-inside elementor-widget elementor-widget-slides\" data-id=\"ee666c7\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;navigation&quot;:&quot;arrows&quot;,&quot;autoplay_speed&quot;:4000,&quot;transition_speed&quot;:4000,&quot;autoplay&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;infinite&quot;:&quot;yes&quot;,&quot;transition&quot;:&quot;slide&quot;}\" data-widget_type=\"slides.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t\t\t<div class=\"elementor-slides-wrapper elementor-main-swiper swiper\" role=\"region\" aria-roledescription=\"carousel\" aria-label=\"Diapositivas\" dir=\"ltr\" data-animation=\"fadeInUp\">\n\t\t\t\t<div class=\"swiper-wrapper elementor-slides\">\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-repeater-item-c97f30c swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"TURBIDI.T\u2122: Modern IoT-Enabled, Modular Turbidimeter.\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div><div class=\"elementor-repeater-item-1ae3858 swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div class=\"swiper-slide-bg\" role=\"img\" aria-label=\"TURBIDI.T\u2122 with Cartridges: Modern IoT-Enabled Modular Turbidimeter.\"><\/div><div class=\"swiper-slide-inner\" ><div class=\"swiper-slide-contents\"><\/div><\/div><\/div><div class=\"elementor-repeater-item-909265f swiper-slide\" role=\"group\" aria-roledescription=\"slide\"><div 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Ponte en contacto con nosotros para pedir un presupuesto o para saber m\u00e1s<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-69d0943 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"69d0943\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/rheolution.com\/es\/contacto\/\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Ponte en contacto con nosotros para m\u00e1s informaci\u00f3n<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-469fdb5 elementor-widget elementor-widget-heading\" data-id=\"469fdb5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Referencias<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-685d6db elementor-widget elementor-widget-text-editor\" data-id=\"685d6db\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>[1]  <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S095816692100152X\" target=\"_blank\" rel=\"noopener\">Reardon, K. F. (2021). Practical monitoring technologies for cells and substrates in biomanufacturing. Current Opinion in Biotechnology, 71, 225-230.  <\/a><\/p><p>[2]  <a href=\"https:\/\/link.springer.com\/article\/10.1186\/s12934-021-01617-z\" target=\"_blank\" rel=\"noopener\">Madhavan, A., Arun, K. B., Sindhu, R., Krishnamoorthy, J., Reshmy, R., Sirohi, R., &#8230; &#038; Binod, P. (2021). Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up. Microbial cell factories, 20(1), 124.  <\/a><\/p><p>[3]<a href=\"https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/\" target=\"_blank\" rel=\"noopener\">Tankeshwar Acharya (2021). Bacterial Culture Media: Classification, Types, Uses. Retrieved from https:\/\/microbeonline.com\/types-of-bacteriological-culture-medium\/.  <\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ec9a286 elementor-widget elementor-widget-heading\" data-id=\"ec9a286\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Art\u00edculos relacionados<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca12d3f premium-blog-cta-full-yes premium-floating-effects-yes premium-disable-fe-yes elementor-widget__width-initial premium-blog-align-left elementor-widget elementor-widget-premium-addon-blog\" data-id=\"ca12d3f\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;force_height&quot;:&quot;true&quot;,&quot;premium_blog_grid&quot;:&quot;yes&quot;,&quot;premium_blog_layout&quot;:&quot;even&quot;,&quot;premium_blog_columns_number&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_tablet&quot;:&quot;50%&quot;,&quot;premium_blog_columns_number_mobile&quot;:&quot;100%&quot;,&quot;scroll_to_offset&quot;:&quot;yes&quot;,&quot;premium_fe_trigger&quot;:&quot;load&quot;,&quot;premium_fe_direction&quot;:&quot;alternate&quot;,&quot;premium_fe_loop&quot;:&quot;default&quot;,&quot;premium_fe_easing&quot;:&quot;easeInOutSine&quot;}\" data-widget_type=\"premium-addon-blog.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t\n\n\t\t\t\t<div class=\"premium-blog-wrap  premium-blog-even\" data-page=\"70939\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65541\" alt=\"Blog Post Image: Methods to Quantify Cell Concentration Using TURBIDI.T\u2122 Technology.\" data-attachment-id=\"65541\" data-permalink=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/cuantificar-la-concentracion-celular-con-turbidi-t\/attachment\/quantifying-cell-concentration-in-culture-media-with-turbidi-t-header-3-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Methods to Quantify Cell Concentration\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/Quantifying-cell-concentration-in-culture-media-with-TURBIDI.T%E2%84%A2-header.jpg?fit=1024%2C384&amp;ssl=1\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/cuantificar-la-concentracion-celular-con-turbidi-t\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">\u00bfC\u00f3mo se mide la concentraci\u00f3n celular con TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/cuantificar-la-concentracion-celular-con-turbidi-t\/\" target=\"_blank\">\n\t\t\t\t\u00bfC\u00f3mo se mide la concentraci\u00f3n celular con TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">La creciente demanda de m\u00e9todos m\u00e1s r\u00e1pidos para cuantificar c\u00e9lulas va m\u00e1s all\u00e1 de las c\u00e1maras de recuento tradicionales, lo que da lugar a soluciones innovadoras que facilitan un recuento preciso y reducen el tiempo necesario. La turbidez, que refleja la opacidad de la soluci\u00f3n debido a la presencia de part\u00edculas que dispersan la luz, se perfila como un m\u00e9todo eficaz para acelerar la cuantificaci\u00f3n celular.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/cuantificar-la-concentracion-celular-con-turbidi-t\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leer m\u00e1s \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t<div class=\"premium-blog-post-outer-container\" data-total=\"1\">\n\t\t\t<div class=\"premium-blog-post-container premium-blog-skin-classic\">\n\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumb-effect-wrapper\">\n\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-container premium-blog-zoomin-effect\">\n\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"1200\" height=\"450\" src=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" class=\"attachment-full size-full wp-image-65599\" alt=\"Measuring liquid particle size with TURBIDI.T\u2122.\" data-attachment-id=\"65599\" data-permalink=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-tamano-de-las-particulas-en-una-muestra-liquida\/attachment\/web_banner_size-4-4\/\" data-orig-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1200%2C450&amp;ssl=1\" data-orig-size=\"1200,450\" data-comments-opened=\"1\" data-image-title=\"Measuring liquid particle\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?fit=1024%2C384&amp;ssl=1\" srcset=\"https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?w=1200&amp;ssl=1 1200w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=300%2C113&amp;ssl=1 300w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=1024%2C384&amp;ssl=1 1024w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=768%2C288&amp;ssl=1 768w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=260%2C98&amp;ssl=1 260w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=50%2C19&amp;ssl=1 50w, https:\/\/i0.wp.com\/rheolution.com\/wp-content\/uploads\/web_banner_size.jpg?resize=150%2C56&amp;ssl=1 150w\" sizes=\"(max-width:767px) 700px, (max-width:1200px) 100vw, 1200px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-thumbnail-overlay\">\n\t\t\t\t\t\t\t\t<a class=\"elementor-icon\" href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-tamano-de-las-particulas-en-una-muestra-liquida\/\" target=\"_blank\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">\u00bfC\u00f3mo se mide el tama\u00f1o de las part\u00edculas en una muestra l\u00edquida con TURBIDI.T\u2122?<\/span>\n\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"premium-blog-content-wrapper \">\n\n\t\t\t\t\t<div class=\"premium-blog-inner-container\">\n\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<h4 class=\"premium-blog-entry-title\">\n\t\t\t<a href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-tamano-de-las-particulas-en-una-muestra-liquida\/\" target=\"_blank\">\n\t\t\t\t\u00bfC\u00f3mo se mide el tama\u00f1o de las part\u00edculas en una muestra l\u00edquida con TURBIDI.T\u2122?\t\t\t<\/a>\n\t\t<\/h4>\n\t\t\t\t<div class=\"premium-blog-entry-meta\">\n\t\t\t\n\t\t\t\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"premium-blog-content-inner-wrapper\">\n\t\t<p class=\"premium-blog-post-content\">La determinaci\u00f3n del tama\u00f1o de las part\u00edculas es importante en muchos campos, como las ciencias de la vida (nanomedicina, administraci\u00f3n de f\u00e1rmacos, ingenier\u00eda de tejidos, bioan\u00e1lisis), la qu\u00edmica, las ciencias ambientales y la industria. La turbidimetr\u00eda es un m\u00e9todo r\u00e1pido y no destructivo para estimar el tama\u00f1o de las part\u00edculas siguiendo un procedimiento muy sencillo.<\/p><div class=\"premium-blog-excerpt-link-wrap\"><a href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-tamano-de-las-particulas-en-una-muestra-liquida\/\" target=\"_blank\" class=\"premium-blog-excerpt-link elementor-button\">Leer m\u00e1s \u00bb<\/a><\/div>\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\t\t\t\t\t\t\t<\/div>\n\n\t\t\n\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>En el \u00e1mbito de la bioingenier\u00eda y la biotecnolog\u00eda, las c\u00e9lulas act\u00faan como catalizadores para la producci\u00f3n de diversos productos valiosos, como biocombustibles, f\u00e1rmacos, anticuerpos, enzimas industriales y productos qu\u00edmicos de origen biol\u00f3gico [1]. En este contexto, la levadura, un microorganismo muy utilizado, desempe\u00f1a un papel fundamental. Controlar el crecimiento de la levadura es fundamental para garantizar unas condiciones \u00f3ptimas y la productividad en los procesos biotecnol\u00f3gicos [2]. Tradicionalmente, se ha utilizado el an\u00e1lisis espectrofotom\u00e9trico para evaluar la densidad \u00f3ptica a 600 nm (DO600 nm) midiendo la absorci\u00f3n de luz por parte de la levadura&#8230;   <\/p>\n","protected":false},"author":195401338,"featured_media":70940,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_wpcom_ai_launchpad_first_post":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[287,320,315],"tags":[123,148,124,121,137],"class_list":["post-70939","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-notas-de-aplicacion","category-turbidi-t-life-sciences","category-turbidit-agrifood","tag-agri-food","tag-comparison-of-technologies","tag-life-sciences","tag-microbiology","tag-agri-food-turbidity"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Seguimiento del Crecimiento de la Levadura: Turbidez en los Biorreactores<\/title>\n<meta name=\"description\" content=\"Utiliza los instrumentos \u00f3pticos de IoT de Rheolution para supervisar con precisi\u00f3n los cultivos celulares y el crecimiento bacteriano mediante mediciones avanzadas de turbidez.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/rheolution.com\/es\/notas-de-aplicacion\/medir-el-crecimiento-de-la-levadura-en-un-biorreactor-mediante-la-turbidez\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Seguimiento del Crecimiento de la Levadura: Turbidez en los Biorreactores\" \/>\n<meta property=\"og:description\" content=\"Utiliza los 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