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This is a short report of a study performed by Shiva Naseri, Newsha Koushki, Ehsan Rezabeigi, Allen Ehrlicher and Showan N. Nazhat at McGill University (Montreal, Canada) which title is A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time (published in 2020 in the Journal of the Mechanical Behavior of Biomedical Materials, 110, 103921).
— The measurement of the absolute blood viscoelastic properties during coagulation is not possible with most conventional techniques.
— ElastoSens™ Bio has shown to provide repeatable and sensitive measurements to test blood coagulation through the viscoelastic properties of the forming clot.
— The concentration of CaCl2 in blood affects the initialization of coagulation (showing a non-linear behavior). The stiffness of the developed clot is proportional to the concentration of CaCl2.
— ElastoSens™ Bio’s measurements were similar to those obtained with a rotational rheometer. The ElastoSens™ Bio’s data showed a better correlation to CaCl2 concentration compared to TEG.
The viscoelastic properties of coagulating blood can be correlated with several diseases and genetic conditions that affect the natural blood coagulation process including bleeding disorders, hemophilia, rare factor deficiencies, von Willebrand disease and platelet function disorders. Therefore, the evaluation of blood clot properties can be valuable for the study, diagnosis and eventually treatment of these diseases. Conventional and more recent techniques used to assess blood coagulation such as thromboelastography (TEG), rotational thromboelastometry (ROTEM) and magnetomotive optical coherence elastography do not directly measure the absolute viscoelasticity of the clot. Furthermore, they normally offer a destructive testing which prevents complementary characterization of the clots and/or the monitoring of the dynamic changes during blood coagulation. Other issues related to these techniques also include low sensitivity and repeatability. In this short application note, ElastoSens™ Bio was used for monitoring the shear storage modulus (G’) of whole blood during coagulation induced by different concentrations of CaCl2 . In addition, similar tests were performed using TEG and rheometer for comparison.
MATERIALS AND METHODS
RESULTS AND DISCUSSION
The increasing concentration of CaCl2 from 15 mM to 35 mM reduced the clotting time while a further increase (to 45 mM) led to a longer reaction time. The higher concentration of CaCl2 resulted in higher clot stiffness. The relation between CaCl2 concentration and the clot final storage moduli measured by the ElastoSens™ Bio was close to those measured by the rheometer. In turn, this information was correlated to the results obtained by TEG (the conventional instrument used in clinics).
ElastoSens™ Bio can directly measure the evolution of viscoelasticity during clot formation. It provides robust measurements and an easy-to-use platform. The instrument can be used for:
— R&D: providing superior quantitative data to better investigate blood coagulation.
— Preclinical studies: to study the effect of medication on blood coagulation.
 Naseri, S., Koushki, N., Rezabeigi, E., Ehrlicher, A., & Nazhat, S. N. (2020). A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time. Journal of the Mechanical Behavior of Biomedical Materials, 110, 103921
 Evans, P. A., Hawkins, K., Lawrence, M., Williams, R. L., Barrow, M. S., Thirumalai, N., & Williams, P. R. (2008). Rheometry and associated techniques for blood coagulation studies. Medical engineering & physics, 30(6), 671-679.
 Whiting, D., & DiNardo, J. A. (2014). TEG and ROTEM: technology and clinical applications. American journal of hematology, 89(2), 228-232.
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