Hydrogels are extensively used for tissue engineering, cell therapy or controlled release of bioactive factors. Non-destructive techniques that can follow their viscoelastic properties during polymerization, remodeling and degradation are needed, since these properties are determinant for their in vivo efficiency. In this work, we proposed the Viscoelastic Testing of Bi-Layered Materials (VeTBiM) as a new method for nondestructive and contact-less mechanical characterization of soft materials. The VeTBiM method measures the dynamic displacement response of a material, to a low amplitude vibration in order to characterize its viscoelastic properties. We validated VeTBiM by comparing data obtained on various agar and chitosan hydrogels with data from rotational rheometry, and compression tests. We then investigated its potential to follow the mechanical properties of chitosan hydrogels during gelation and in the presence of papain and lysozyme that induce fast or slow enzymatic degradation. Thanks to this nondestructive and contactless approach, samples can be removed from the instrument and stored in different conditions between measurements. VeTBiM is well adapted to follow biomaterials alone or with cells, over long periods of time. This new method will help in the fine-tuning of the mechanical properties of biomaterials used for cell therapy and tissue engineering.