Hydrogels are extensively used for tissue engineering and regenerative medicine. Their mechanical properties determine not only their function but also cell response (i.e. proliferation and differentiation of stem cells) and their degradation kinetics is particularly important to predict scaffold resorption, new tissue formation and integration after implantation. Non-destructive methods are needed to screen and follow long-term evolution of rheological properties. In this work, we demonstrate the potential of a new non-destructive instrument, ElastoSens™ Bio2 (Rheolution Inc., Montréal, QC), that measures in real-time and without contact the evolution of rheological properties of chitosan hydrogels during network formation and degradation during exposure to human lysozyme.