News / Rheolution Latest News / What is Viscoelasticity?
— The precise analysis of hemostatic agents (HA) in contact with blood are not possible with most conventional testing techniques.
— ElastoSens™ Bio successfully measured the coagulation of blood in presence of HA through the clot viscoelastic properties.
— It is shown here that increasing the concentration of HA in blood may affect the clot initiation time, clot strength and volume (swelling) depending on the mode of action (MoA) of the HA.
— ElastoSens™ Bio can be used for R&D and development of HA, QC & manufacturing and for preclinical studies.
MATERIALS AND METHODS
CELOX™ (MedTrade Products Ltd., Crewe, UK) and QuikClot® (Z-MEDICA, LLC, Wallingford, CT, USA) were added into the ElastoSens™ Bio sample holders at different powder/blood weight dosages: 0 %, 5 % and 10 % (w/w) for CELOX™ and 5 %, 10 % and 15 % (w/w) for QuikClot®. Sample holders and hemostatic agents were pre-heated at 37 °C into the thermal chamber of the instrument. Whole sheep blood in anticoagulants (Sodium Citrate) from Cedarlane (Burlington, ON, Canada) was heated at 37 °C in a water bath and then re-calcified by mixing with CaCl2. A volume of 5 mL of re-calcified blood was then pipetted and introduced into the sample holders containing the HA and the test was initiated. The test was performed at 37 °C during 40 minutes for CELOX™ and 20 minutes for QuikClot®. The ElastoSens™ Bio was used to measure in real time the shear storage modulus (G’) of the clot during its formation as well as the sample swelling ratio calculated as follows:
RESULTS AND DISCUSSION
 Wheat, J. C., & Wolf, J. S. (2009). Advances in bioadhesives, tissue sealants, and hemostatic agents. Urologic Clinics, 36(2), 265-275.
 Li, J., Cao, W., Lv, X. X., Jiang, L., Li, Y. J., Li, W. Z., … & Li, X. Y. (2013). Zeolite-based hemostat QuikClot releases calcium into blood and promotes blood coagulation in vitro. Acta Pharmacologica Sinica, 34(3), 367-372.
 Khoshmohabat, H., Paydar, S., Kazemi, H. M., & Dalfardi, B. (2016). Overview of agents used for emergency hemostasis. Trauma monthly, 21(1).
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